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Junctional membrane-targeting mechanism of cardiac L-type calcium channel

Research Project

Project/Area Number 22790206
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field General physiology
Research InstitutionShinshu University

Principal Investigator

NAKADA Tsutomu  信州大学, 医学部, 助教 (70452141)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsL型カルシウムチャネル / リアノジン受容体 / 心筋 / 生理学 / 循環器・高血圧 / カプロン
Research Abstract

In cardiac myocytes, L-type calcium channels(LTCC) form a functional signaling complex with ryanodine receptors at the junctional membrane(JM). Although the specific localization of LTCCs to JM is critical for excitation-contraction coupling, their targeting mechanism is unclear. To identify the sequences of a_<1C> responsible for the JM-targeting, we generated various mutants of aic subunit, and studied their targeting properties in dysgenic(a_<1S.-null) GLT myotubes. The results revealed that amino acids^<1681> LQAGLRTL^<1688> and^<1693> PEIRRAIS^<1700> predicted to form two adjacent a-helices in the proximal C-terminus are necessary for the JM-targeting of a_<1C>.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (8 results)

All 2012 2011 2010

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (4 results)

  • [Journal Article] Chronic receptor-mediated activation of Gi/o proteins alters basal t-tubular and sarcolemmal L-type Ca^<2+> channel activity through phosphatases in heart failure2012

    • Author(s)
      Kashihara, T., Nakada, T., Shimojo, H., Horiuchi-Hirose, M., Gomi, S., Shibazaki, T., Sheng, X., Hirose, M., Hongo, M., and Yamada, M
    • Journal Title

      Am. J. Physiol. Heart Circ. Physiol

      Volume: 302 Issue: 8 Pages: 1645-1654

    • DOI

      10.1152/ajpheart.00589.2011

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Diacylglycerol KinaseζInhibits Ventricular Tachyarrhythmias in a Mouse Model of Heart Failure2011

    • Author(s)
      Hirose M, Takeishi Y, Niizeki T, Nakada T, Shimojo H, Kashihara T, Horiuchi-Hirose M, Kubota I, Mende U, Yamada M.
    • Journal Title

      Circ. J

      Volume: 75 Pages: 2333-2342

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Diacylglycerol Kinase &zeta; Inhibits Ventricular Tachyarrhythmias in a Mouse Model of Heart Failure2011

    • Author(s)
      Hirose, M., Takeishi, Y., Niizeki, T., Nakada, T., Shimojo, H., Kashihara, T., Horiuchi-Hirose, M., Kubota, I., Mende, U., Yamada, M
    • Journal Title

      Circulation Journal

      Volume: 75 Issue: 10 Pages: 2333-2342

    • DOI

      10.1253/circj.CJ-10-1213

    • NAID

      10029459214

    • ISSN
      1346-9843, 1347-4820
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Decrease in the density of t-tubular L-type Ca^<2+> channel currents in failing ventricular myocytes2011

    • Author(s)
      Horiuchi-Hirose M, Kashihara T, Nakada T, Kurebayashi N, Shimojo H, Shibazaki T, Sheng X, Yano S, Hirose M, Hongo M, Sakurai T, Moriizumi T, Ueda H, Yamada M
    • Journal Title

      Am.J.Physiol.Heart Circ.Physiol.

      Volume: 300

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] L型カルシウムチャネルとリアノジン受容体の結合膜局在化におけるジャンクトフィリンの役割2012

    • Author(s)
      中田勉, Bernhard E.Flucher, 柏原俊英, 生暁娜, 柴崎利英, 五味志文, 山田充彦
    • Organizer
      第85回日本薬理学会年会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Proximal C-terminus of alC subunits is necessary for junctional membrane-targeting of cardiac L-type calcium channels2011

    • Author(s)
      Tsutomu Nakada, Bernhard E.Flucher, Toshihide Kashihara, Xiaona Sheng, Toshihide Shibazaki, Miwa Horiuchi-Hirose, Masamichi Hirose, Mitsuhiko Yamada
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] 心筋L型カルシウムチャネルの結合膜局在化モチーフの探索2011

    • Author(s)
      中田勉, Bernhard E Flucher,生暁梛,柏原俊英,柴崎利英,山田充彦
    • Organizer
      第84回日本薬理学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2011-03-16
    • Related Report
      2011 Final Research Report
  • [Presentation] GLT細胞を用いた心筋L型Ca^<2+>チャネルのクラスタリング機構の解析2010

    • Author(s)
      中田勉, Bernhard E Flucher, 生暁娜, 柏原俊英, 柴崎利英, 山田充彦
    • Organizer
      第123回日本薬理学会関東部会
    • Place of Presentation
      栃木
    • Year and Date
      2010-10-23
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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