Abnormal circulatory function in TRIC-A-knockout mice

• Project/Area Number: 22790209
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General physiology
• Research Institution: Kyoto University
• Principal Investigator: YAMAZAKI Daiju 京都大学, 生理化学研究ユニット, 特定講師 (40467428)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 微小循環 / 末梢循環 / 循環力学 / 循環調節 / カウンターイオン / カルシウム / TRICチャネル / 小胞体 / 高血圧 / 心肥大 / 循環機能 / リアノジン受容体(RyR) / イノシトール3リン酸受容体(IP3R) / 徐脈 / 血管トーヌス

Research Abstract

The aim of this project is to examine the pathogenic mechanism of abnormal circulatory function such as hypertension and isoproterenol(Iso) induced hypertrophy in TRIC-A-knockout mice. It was found that hypertension was caused by abnormality of vascular tonus regulation in TRIC-A-knockout vascular smooth muscle cells. On the other hand, cardiac fibrosis but not hypertrophy was significantly induced by Iso infusion using osmotic pump in TRIC-A-knockout mice. Unfortunately, it was unclear the mechanism of cardiac fibrosis during research period. We will try to examine this project in another one.

Research Products

• [Journal Article] TRIC-A channels in vascular smooth muscle contribute to blood pressure maintenance (2011)
  • Author(s): Yamazaki D, Tabara Y, Kita S, Hanada H, Komazaki S, Naitou D, Mishima A, Nishi M, Yamamura H, Yamamoto S, Kakizawa S, Miyachi H, Yamamoto S, Miyata T, Kawano Y, Kamide K, Ogihara T, Hata A, Umemura S, Soma M, Takahashi N, Imaizumi Y, Miki T, Iwamoto T, Ta
  • Journal Title: Cell Metab Volume: 14 Issue: 2 Pages: 231-241
  • DOI: 10.1016/j.cmet.2011.05.011
  • NAID: 120003255678
  • URL: http://hdl.handle.net/2433/143634
  • Peer Reviewed
• [Journal Article] Up-regulation of Kir2.1 by ER stress facilitates cell death of brain capillary endothelial cells (2011)
  • Author(s): Kito H, Yamazaki D, Ohya S, 他3名
  • Journal Title: Biophysical & Biochemical Research Communications Volume: 411巻 Issue: 2 Pages: 293-298
  • DOI: 10.1016/j.bbrc.2011.06.128
  • Peer Reviewed
• [Journal Article] Molecular architecture of Ca^<2+> signaling control in muscle and heart cells (2011)
  • Author(s): Zhao X, Yamazaki D, Kakizawa S, Pan Z, Takeshima H & Ma J.
  • Journal Title: Channels Volume: 5 Issue: 5 Pages: 389-394
  • DOI: 10.4161/chan.5.5.16467
  • Peer Reviewed
• [Journal Article] Contribution of Kir2 potassium channels toATP-induced cell death in brain capillary endothelial cells and reconstitutedHEK293 cell model (2011)
  • Author(s): Yamazaki D, Kito H, Yamamoto S, Ohya S, Yamamura H, Asai K & Imaizumi Y.
  • Journal Title: American Journal of Physiology Cell Physiology Volume: 300
  • Peer Reviewed
• [Journal Article] Ca2+ overload and sarcoplasmic reticulum instability in tric-a null skeletal muscle (2010)
  • Author(s): Zhao, X., Yamazaki, D., Park, KH., Komazaki, S., Tjondrokoesoemo, A., Nishi, M., Lin, P., Hirata, Y., Brotto, M., Takeshima, H & Ma, J.
  • Journal Title: J. Biol. Chem Volume: 285 Issue: 48 Pages: 37370-37376
  • DOI: 10.1074/jbc.m110.170084
  • Peer Reviewed
• [Journal Article] two ion-channels, TRIC-A and TRIC-B, masquerade as a single K+-channel (2010)
  • Author(s): Pitt, S. J., Park, KH, Nishi M., Urashima, T., Aoki, S., Yamazaki, D., Ma, J., Takeshima, H. & Sitsapesan R. Charade of the SR K+-channel
  • Journal Title: Biophys. J. Volume: 99 Issue: 2 Pages: 417-426
  • DOI: 10.1016/j.bpj.2010.04.051
  • Peer Reviewed
• [Journal Article] 小胞体Ca^<2+>放出におけるTRIC (2010)
  • Author(s): 山崎大樹、山本伸一郎、竹島浩
  • Journal Title: チャネルの生理的役割日薬理誌 Volume: 135 Pages: 99-103
• [Journal Article] 小胞体におけるTRICチャネル機構 (2010)
  • Author(s): 山崎大樹
  • Journal Title: 医学のあゆみ Volume: vol.235 Pages: 341-341
• [Presentation] ArrhythmicCa2+signaling in the heart of Tric-a-knockout mice. (2012)
  • Author(s): Yamazaki D and Takeshima H
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 長野県松本文化会館(長野県)
  • Year and Date: 2012-03-30
• [Presentation] Arrhythmic Ca^<2+> signaling in the heart of Tric-a-knockout mice (2012)
  • Author(s): Daiju Yamazaki, Hiroshi Takeshima
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 長野県松本文化会館(長野県)
  • Year and Date: 2012-03-30
• [Presentation] 血管平滑筋におけるTRIC-Aの血圧維持機構 (2012)
  • Author(s): 山崎大樹喜多紗斗美、西美幸、今泉祐治、岩本隆宏、竹島浩
  • Organizer: 第85回日本薬理学会年会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2012-03-14
• [Presentation] Arrhythmic intracellular Ca^<2+> signaling and electrocardiogram in the heart of Tric-a-/-mice. (2012)
  • Author(s): Park KH, Yamazaki D, Naitou D, Zhao C, Pan Z, Takeshima H, Ma J.
  • Organizer: Biophysical Society 56^<th> annual meeting
  • Place of Presentation: San Diego(USA)
  • Year and Date: 2012-02-26
• [Presentation] Arrhythmic Intracellular Ca^<2+> signaling and Electrocardiogramin in the heart of Tric-a-/-mice (2012)
  • Author(s): Park KH, Yamazaki D, et al
  • Organizer: Biophysical Society 56th Annual Meeting
  • Place of Presentation: サンディエゴ(アメリカ)
  • Year and Date: 2012-02-26
• [Presentation] TRIC-A Gene polymorphism associated with hypertension patients. (2011)
  • Author(s): Zhao C, Yamazaki D, Tabara Y, Hanada H, Nishi M, Miyata T, Miki T, Takeshima H.
  • Organizer: 8^<th> AFMC International Medicine Chemistry Symposium
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2011-12-01
• [Presentation] TRIC-Achannels in vascular smooth muscle contribute to blood pressure maintenance. (2011)
  • Author(s): Yamazaki, Kita S, Naitou D, Nishi M, Imaizumi Y, Iwamoto T, Takeshima H.
  • Organizer: 8^<th> AFMC International Medicine Chemistry Symposium
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2011-12-01
• [Presentation] TRIC-A Channels in Vascular Smooth Muscle Contribute to Blood Pressure Maintenance (2011)
  • Author(s): Yamazaki D, Kita S, Naitou D, et al
  • Organizer: 8th AFMC International Medicine Chemistry Symposium
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2011-12-01
• [Presentation] TRIC-A-Gene Polymorphism Associated with Hypertension Patients (2011)
  • Author(s): Zhao C, Yamazaki D, Tabara Y, et al
  • Organizer: 8th AFMC International Medicine Chemistry Symposium
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2011-12-01
• [Presentation] TRIC-Aチャネルは血管平滑筋において血管調節に寄与する. (2011)
  • Author(s): 山崎大樹、喜多紗斗美、内藤大督、西美幸、今泉祐治、岩本隆宏、竹島浩.
  • Organizer: 第61回日本薬学会近畿支部総会
  • Place of Presentation: 神戸学院大学(兵庫県)
  • Year and Date: 2011-10-22
• [Presentation] TRIC-Aチャネルは血管平滑筋において血圧調節に寄与する (2011)
  • Author(s): 山崎大樹、喜多紗斗美、内藤大督, ら
  • Organizer: 第61回日本薬学会近畿支部総会・大会
  • Place of Presentation: 神戸学院大学
  • Year and Date: 2011-10-22
• [Presentation] 血管平滑筋におけるTRIC-Aチャネルは血圧調節に寄与している (2011)
  • Author(s): 内藤大督、山崎大樹、竹島浩
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2011-09-24
• [Presentation] TRIC-Achannel and cardiac hypertrophy (2011)
  • Author(s): Tao S, Yamazaki D, Takeshima H.
  • Organizer: The 6th SKO Joint Symposium.
  • Place of Presentation: Seoul National University(Korea)
  • Year and Date: 2011-06-02
• [Presentation] TRIC-A channel and cardiac hypertrophy (2011)
  • Author(s): Tao S, Yamazaki D, Takeshima H
  • Organizer: The 6th SKO Joint Symposium
  • Place of Presentation: Seoul National Univ.(韓国)
  • Year and Date: 2011-06-02
• [Presentation] TRIC-A channel and blood pressure regulation. (2011)
  • Author(s): Yamazaki D, Kita S, Komazaki S, NaitouD, Nishi M, Yamamura H, Imaizumi Y, Iwamoto T, Takeshima H.
  • Organizer: Biophysical Society 55^<th> annual meeting.
  • Place of Presentation: Baltimore(USA)
  • Year and Date: 2011-03-07
• [Presentation] TRIC-A channel and blood pressure regulation (2011)
  • Author(s): Yamazaki, et al.
  • Organizer: Biophysical Society, 55^<th> Annual Meeting
  • Place of Presentation: Baltimore, Maryland (USA)
  • Year and Date: 2011-03-07
• [Presentation] イオンチャネル・トランスポーターと心血管機能:細胞機能の分子機序とその統合的理解 (2010)
  • Author(s): 山崎大樹、竹島浩.
  • Organizer: 平成22年度生理研研究会
  • Place of Presentation: 岡崎カンファレンスセンター(愛知県)
  • Year and Date: 2010-11-05
• [Presentation] Ca2+signaling dysfunction in embryonic cardiomyocytes of lacking RyR, JP or TRI Cchannels. (2010)
  • Author(s): Yamazaki D and Takeshima H.
  • Organizer: XX World Congress ISHR 2010 Kyoto
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2010-05-31
• [Presentation] TRIC channel function in sarco/endoplasmic reticulum. (2010)
  • Author(s): Yamazaki D, Yamamoto S, Nishi M, Takeshima H.
  • Organizer: 第87回日本生理学会大会.
  • Place of Presentation: 盛岡市民文化ホール(岩手県)
  • Year and Date: 2010-05-21
• [Remarks] 京都大学研究成果発表
  • URL: http://www.kyoto-u.ac.jp/ja/news_data/h/h1/news6/2011/110802_1.htm
• [Remarks] 京都大学薬学研究科生体分子認識学分野
  • URL: http://www.pharm.kyoto-u.ac.jp/biochem/news26.htm
• [Remarks]
  • URL: http://www.kyoto-u.ac.jp/ja/news_data/h/h1/news6/2011/110802_1.htm
• [Remarks]
  • URL: http://www.pharm.kyoto-u.ac.jp/biochem/news26.htm