The Dynamic mechanism of phagocytosis of apoptotic cells

• Project/Area Number: 22790210
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General physiology
• Research Institution: Osaka University (2011-2012); Kyoto University (2010)
• Principal Investigator: HANAYAMA Rikinari 大阪大学, 免疫学フロンティア研究センター, 特任准教授 (40403191)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: アポトーシス / マクロファージ / 貪食 / Tim4 / MFG-E8 / TIM4 / Dock180 / ライブイメージング

Research Abstract

Apoptotic cells are engulfed by macrophages by recognizing phosphatidylserine (PS). We found that Tim4 receptor on macrophages tethers apoptotic cells to macrophages by binding PS, and MFG-E8 secreted from macrophages binds to PS and stimulates the engulfment by binding to integrin on macrophages.

Research Products

• [Journal Article] Two-step engulfment of apoptotic cells (2012)
  • Author(s): Toda S, Hanayama R, Nagata S
  • Journal Title: Molecular and Cellular Biology Volume: 32(1): 118-125
  • NAID: 120005300448
  • Peer Reviewed
• [Journal Article] The Angelman Syndrome protein Ube3A regulates synapse development by ubiquitinating Arc (2010)
  • Author(s): Greer PL, Hanayama R, Greenberg ME
  • Journal Title: Cell Volume: 140 Pages: 704-716
  • Peer Reviewed
• [Journal Article] Autoimmunity and the clearance of dead cells (2010)
  • Author(s): Nagata S, Hanayama R, Kawane K
  • Journal Title: Cell Volume: 140 Pages: 619-630
  • Peer Reviewed
• [Journal Article] The Angelman Syndrome protein Ube3A regulates synapse development by ubiquitinating arc. (2010)
  • Author(s): Greer PL, Hanayama R(equal contribution), et al.
  • Journal Title: Cell Volume: 140(5) Pages: 704-716
  • Peer Reviewed
• [Journal Article] Autoimmunity and the clearance of dead cells. (2010)
  • Author(s): Nagata S, Hanayama R, Kawane K
  • Journal Title: Cell Volume: 140(5) Pages: 619-630
  • Peer Reviewed
• [Presentation] Identification of a novel regulator for lysosomal fusion in macrophages (2013)
  • Author(s): 華山力成
  • Organizer: 第2回御茶ノ水動脈硬化フォーラム
  • Place of Presentation: 庭のホテル(東京)
  • Year and Date: 2013-02-23
• [Presentation] Identification of a novel regulator for lysosomal fusion in macrophages. (2013)
  • Author(s): 華山力成
  • Organizer: 第2回 御茶ノ水動脈硬化フォーラム
  • Place of Presentation: 庭のホテル（東京）
  • Invited
• [Presentation] Engulfment of apoptotic cells and its defects (2011)
  • Author(s): 華山力成
  • Organizer: シンフォニーシンポジウム
  • Place of Presentation: ホテルメトロポリタンエドモント(東京)
  • Year and Date: 2011-09-11
• [Presentation] Engulfment of apoptotic cells and its defects (2011)
  • Author(s): 華山力成
  • Organizer: Symphony symposium
  • Place of Presentation: ホテルメトロポリタンエドモント(東京都)(招待講演)
  • Year and Date: 2011-09-11
• [Presentation] 死細胞の除去とその疾患 (2010)
  • Author(s): 華山力成
  • Organizer: 阿波シンポジウム
  • Place of Presentation: 徳島大学
  • Year and Date: 2010-08-11
• [Remarks]
  • URL: http://immnet.ifrec.osaka-u.ac.jp
• [Remarks]
  • URL: http://www2.mfour.med.kyoto-u.ac.jp/~nagata/