Chloride dynamics in beating cardiomyocytes

• Project/Area Number: 22790211
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General physiology
• Research Institution: Kyoto University
• Principal Investigator: KIM Uongju 京都大学, 医学研究科, 特定研究員 (80511823)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 未熟・成熟B細胞 / ミトコンドリア / 膜結合型B細胞レセプター / ミトコンドリアCa2+輸送体 / 細胞質Ca2+ / ミトコンドリアCa^<2+>輸送体 / 細胞質Ca^<2+>

Research Abstract

The purpose of the present study was to clarify the role of mitochondrial NCX in BCR-mediated Ca2+response on B lymphocytes. To achieve these goals, cell physiological experiments were performed and the following results were obtained. 1. Technique to measure the mitochondria and ER Ca2+was established. 2. Expression of NCLX, the mitochondrial NCX, was analyzed in DT40 B cells. 3. Mitochondrial NCX supports ER Ca2+filling and pivotal in BCR-mediated Ca2+signaling in B lymphocytes.

Research Products

• [Journal Article] NHE-1 blockade reversed changes in calcium transient in myocardialslices from isoproterenol-inducedhypertrophied rat left ventricle (2012)
  • Author(s): Hattori H, Takeshita D, Takeuchi A, Kim B, Shibata M, Matsuoka S, Obata K, Mitsuyama S, Zhang GX, Takaki M.
  • Journal Title: Biochem Biophys Res Commun2012 Volume: 419 Pages: 431-5
  • Peer Reviewed
• [Journal Article] Pivotal role of mitochondrial Na+-Ca2+exchange in antigen receptor mediated Ca2+signalling in DT40 and A20 Blymphocytes (2012)
  • Author(s): Kim B, Takeuchi A, Koga O, Hikida M, and Satoshi Matsuoka.
  • Journal Title: J Physiol 2012 Volume: 590 Pages: 459-474
  • Peer Reviewed
• [Journal Article] Pivotal role of mitochondrial Na+-Ca2+ exchange in antigen receptor mediated Ca2+ signalling in DT40 and A20 B lymphocytes (2012)
  • Author(s): Bongju Kim, Ayako Takeuchi, Orie Koga, Masaki Hikida, Satoshi Matsuoka
  • Journal Title: J Physiol Volume: 590 Issue: 3 Pages: 459-474
  • DOI: 10.1113/jphysiol.2011.222927
  • Peer Reviewed
• [Journal Article] NHE-1 blockade reversed changes in calcium transient in myocardial slices from isoproterenol-induced hypertrophied rat left ventricle (2012)
  • Author(s): Hattori H, Obata K, Takaki M, 他
  • Journal Title: Biochem Biophys Res Commun Volume: 419 Issue: 2 Pages: 431-435
  • DOI: 10.1016/j.bbrc.2012.02.041
  • Peer Reviewed
• [Journal Article] Requirement of interaction between mast cells and skin dendritic cells to establish contact hypersensitivity (2011)
  • Author(s): Otsuka A, Kubo M, Honda T, Egawa G, Nakajima S, Tanizaki H, Kim B, Matsuoka S, Watanabe T, Nakae S, Miyachi Y, Kabashima K.
  • Journal Title: PLos one
  • Peer Reviewed
• [Journal Article] Requirement of interaction between mast cells and skin dendritic cells to establish contact hypersensitivity (2011)
  • Author(s): 大塚篤司、中島沙恵子, 他
  • Journal Title: Plos One Volume: 6 Issue: 9 Pages: 229-233
  • DOI: 10.1371/journal.pone.0025538
  • Peer Reviewed
• [Presentation] Role of mitochondrial NCXon CXCL12-induced chemotaxis in Blymphocytes (2012)
  • Author(s): Bongju Kim
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 長野
  • Year and Date: 2012-03-30
• [Presentation] Role of mitochondrial Na-Ca exchange on BCR-mediated Ca2+signalling in B lymphocytes (2012)
  • Author(s): Bongju Kim.
  • Organizer: Biophysical Society 56th Annual Meeting
  • Place of Presentation: San Diego
  • Year and Date: 2012-02-28
• [Presentation] Role of mitochondrial Na-Caexchange on B cell Receptor-mediatedCa2+signaling in DT40 and A20 Blymphocytes (2011)
  • Author(s): Bongju Kim.
  • Organizer: 2011年日光シンポジウム
  • Place of Presentation: 日光
  • Year and Date: 2011-12-17
• [Presentation] Role of mitochondrial Na-Caexchange on BCR-mediated Ca2+signaling in B lymphocytes (2011)
  • Author(s): Bongju Kim
  • Organizer: 第88回日本生理学会大会/第116回日本解剖学会総会・全国学術集会合同大会
  • Place of Presentation: 横浜(震災のため誌上開催)
  • Year and Date: 2011-03-29
• [Presentation] Bリンパ球抗原受容体刺激に伴う細胞内Ca^<2+>反応に対するミトコンドリアNCXの役割 (2011)
  • Author(s): 金鳳柱、竹内綾子、古賀織絵、疋田正喜、松岡達
  • Organizer: 第88回生理学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-28
• [Presentation] リンパ球抗原受容体刺激で誘発される細胞内Ca^<2+>応答のシミュレーション解析 (2011)
  • Author(s): 松岡達、金鳳柱、竹内綾子、古賀織絵、疋田正喜
  • Organizer: 第88回生理学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-28
• [Presentation] Role of mitochondrial Na-Ca exchange on B cell Receptor-mediated Ca2+ signaling in DT40 and A20 B lymphocytes (2011)
  • Author(s): Bongju Kim, Ayako Takeuchi, Orie Koga, Masaki Hikida, Satoshi Matsuoka
  • Organizer: 2011年日光シンポジウム
  • Place of Presentation: 日光東照宮晃陽苑
• [Presentation] リンパ球抗原受容体刺激で誘発される細胞内Ca2+増加に対するミトコンドリアNCXの役割 (2010)
  • Author(s): 金鳳柱
  • Organizer: 第103回近畿生理学談話会
  • Place of Presentation: 大阪
  • Year and Date: 2010-10-02
• [Presentation] リンパ球抗原受容体刺激で誘発される細胞内Ca^<2+>増加に対するミトコンドリアNCXの役割 (2010)
  • Author(s): 金鳳柱、竹内綾子、古賀織絵、疋田正喜、松岡達
  • Organizer: 第103回近畿生理学談話会
  • Place of Presentation: 大阪大学 銀杏会館
  • Year and Date: 2010-10-02