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The analysis of voltage-gated proton channel regulating NADPH oxidase activity

Research Project

Project/Area Number 22790212
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field General physiology
Research InstitutionOsaka University

Principal Investigator

OKOCHI Yoshifumi  大阪大学, 大学院・医学系研究科, 助教 (90435818)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsプロトンチャネル / 活性酸素 / NADPHオキシダーゼ / 脱顆粒 / 電位依存性プロトンチャネル / 貪食細胞
Research Abstract

Voltage-gated proton channel VSOP regulates reactive oxygen species(ROS) in phagocytes. In this study, we found that VSOP deficient neutrophils release azurophilic granules excessively, which contain many kinds of degraded enzyme and myeloperoxidase(MPO) that catalyzes H2O2 to HOCl. The excessive release of azurophilic granules was dependent on the NADPH oxidase activity, which is enzyme to produce superoxide anion. These results suggest that VSOP negatively regulates the release of azurophilic granules to prevent tissue damage by degraded enzyme and HOCl.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (14 results)

All 2012 2011 2010 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (5 results) Remarks (3 results)

  • [Journal Article] Novel and conserved protein macoilin is required for diverse neuronal functions in Caenorhabditis elegans2011

    • Author(s)
      Miyara A, Ohta A, Okochi Y, Tsukada Y, Kuhara A, Mori I
    • Journal Title

      PLoS Genet

      Volume: 7(5)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Bidirectional regulation of thermotaxis by glutamate transmissions in Caenorhabditis elegans2011

    • Author(s)
      Ohnishi N, Kuhara A, Nakamura F, Okochi Y, Mori I
    • Journal Title

      EMBO J

      Volume: 30(7) Pages: 1376-88

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Novel and Conserved Protein Macoilin Is Required for Diverse Neuronal Functions in Caenorhabditis elegans2011

    • Author(s)
      Miyara, A., Ohta, A., Okochi, Y., Tsukada, Y., Kuhara, A., Mori, I.
    • Journal Title

      PLoS Genetics

      Volume: VOL.7 Issue: 5 Pages: 1-14

    • DOI

      10.1371/journal.pgen.1001384

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bidirectional regulation of thermotaxis by glutamate transmissions in Caenorhabditis elegans2011

    • Author(s)
      Ohnishi N, Kuhara A, Nakamura F, Okochi Y, Mori I
    • Journal Title

      EMBO Journal

      Volume: 30 Pages: 1376-88

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reversal of Salt Preference is Directed by the Insulin/ PI3K and Gq/ PKC Signaling in Caenorhabditis elegans2010

    • Author(s)
      Adachi T, Kunitomo H, Tomioka M, Ohno H, Okochi Y, Mori I, * Iino Y
    • Journal Title

      Genetics

      Volume: 186(4) Pages: 1309-19

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reversal of salt preference is directed by the insulin/PI3K and Gq/PKC signaling in Caenorhabditis elegans2010

    • Author(s)
      Adachi T, Kunitomo H, Tomioka M, Ohno H, Okochi Y, Mori I, Iino Y
    • Journal Title

      Genetics

      Volume: 186 Pages: 1309-19

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] The role of voltage-gated proton channel VSOP in host defense against pathogen2012

    • Author(s)
      Yoshifumi Okochi, Yasuaki Aratani, Kazuo Suzuki, Nana Miyawaki, Mari Sasaki, Yasushi Okamura
    • Organizer
      第89回日本生理学会大会
    • Place of Presentation
      松本
    • Year and Date
      2012-03-29
    • Related Report
      2011 Final Research Report
  • [Presentation] The role of voltage-gated proton channel VSOP in host defense against pathogen2012

    • Author(s)
      Yoshifumi Okochi, Yasuaki Aratani, Kazuo Suzuki, Nana Miyawaki, Mari Sasaki, Yasushi Okamura
    • Organizer
      第89回日本生理学会大会
    • Place of Presentation
      長野県松本文化会館
    • Year and Date
      2012-03-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] Regulation of ROS production by VSOP is a voltage-gated proton channel that functions in phagocytes2011

    • Author(s)
      Yoshifumi Okochi, Tsukasa Kawahara、Yasushi Okamura
    • Organizer
      第88回日本生理学会大会
    • Place of Presentation
      横浜
    • Year and Date
      2011-03-28
    • Related Report
      2011 Final Research Report
  • [Presentation] 貧食細胞における電位依存性プロトンチャネルVSOPによる活性酸素産生制御の解析2011

    • Author(s)
      大河内善史、岡村康司
    • Organizer
      第88回日本生理学会大会
    • Place of Presentation
      神奈川(横浜)
    • Year and Date
      2011-03-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] The role of VSOP is a voltage-gated proton channel that functions in phagocytes2010

    • Author(s)
      Yoshifumi Okochi, Tsukasa Kawahara and Yasushi Okamura
    • Organizer
      第87回日本生理学会大会
    • Place of Presentation
      盛岡
    • Year and Date
      2010-05-20
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.med.osaka-u.ac.jp/pub/phys2/okamura/index.html

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.med.osaka-u.ac.jp/pub/phys2/okamura/index.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.med.osaka-u.ac.jp/pub/phys2/tougouseiri/menu.htm

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2016-04-21  

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