The analysis of voltage-gated proton channel regulating NADPH oxidase activity

• Project/Area Number: 22790212
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General physiology
• Research Institution: Osaka University
• Principal Investigator: OKOCHI Yoshifumi 大阪大学, 大学院・医学系研究科, 助教 (90435818)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: プロトンチャネル / 活性酸素 / NADPHオキシダーゼ / 脱顆粒 / 電位依存性プロトンチャネル / 貪食細胞

Research Abstract

Voltage-gated proton channel VSOP regulates reactive oxygen species(ROS) in phagocytes. In this study, we found that VSOP deficient neutrophils release azurophilic granules excessively, which contain many kinds of degraded enzyme and myeloperoxidase(MPO) that catalyzes H2O2 to HOCl. The excessive release of azurophilic granules was dependent on the NADPH oxidase activity, which is enzyme to produce superoxide anion. These results suggest that VSOP negatively regulates the release of azurophilic granules to prevent tissue damage by degraded enzyme and HOCl.

Research Products

• [Journal Article] Novel and conserved protein macoilin is required for diverse neuronal functions in Caenorhabditis elegans (2011)
  • Author(s): Miyara A, Ohta A, Okochi Y, Tsukada Y, Kuhara A, Mori I
  • Journal Title: PLoS Genet Volume: 7(5)
  • Peer Reviewed
• [Journal Article] Bidirectional regulation of thermotaxis by glutamate transmissions in Caenorhabditis elegans (2011)
  • Author(s): Ohnishi N, Kuhara A, Nakamura F, Okochi Y, Mori I
  • Journal Title: EMBO J Volume: 30(7) Pages: 1376-88
  • Peer Reviewed
• [Journal Article] Novel and Conserved Protein Macoilin Is Required for Diverse Neuronal Functions in Caenorhabditis elegans (2011)
  • Author(s): Miyara, A., Ohta, A., Okochi, Y., Tsukada, Y., Kuhara, A., Mori, I.
  • Journal Title: PLoS Genetics Volume: VOL.7 Issue: 5 Pages: 1-14
  • DOI: 10.1371/journal.pgen.1001384
  • Peer Reviewed
• [Journal Article] Bidirectional regulation of thermotaxis by glutamate transmissions in Caenorhabditis elegans (2011)
  • Author(s): Ohnishi N, Kuhara A, Nakamura F, Okochi Y, Mori I
  • Journal Title: EMBO Journal Volume: 30 Pages: 1376-88
  • Peer Reviewed
• [Journal Article] Reversal of Salt Preference is Directed by the Insulin/ PI3K and Gq/ PKC Signaling in Caenorhabditis elegans (2010)
  • Author(s): Adachi T, Kunitomo H, Tomioka M, Ohno H, Okochi Y, Mori I, * Iino Y
  • Journal Title: Genetics Volume: 186(4) Pages: 1309-19
  • Peer Reviewed
• [Journal Article] Reversal of salt preference is directed by the insulin/PI3K and Gq/PKC signaling in Caenorhabditis elegans (2010)
  • Author(s): Adachi T, Kunitomo H, Tomioka M, Ohno H, Okochi Y, Mori I, Iino Y
  • Journal Title: Genetics Volume: 186 Pages: 1309-19
  • Peer Reviewed
• [Presentation] The role of voltage-gated proton channel VSOP in host defense against pathogen (2012)
  • Author(s): Yoshifumi Okochi, Yasuaki Aratani, Kazuo Suzuki, Nana Miyawaki, Mari Sasaki, Yasushi Okamura
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 松本
  • Year and Date: 2012-03-29
• [Presentation] The role of voltage-gated proton channel VSOP in host defense against pathogen (2012)
  • Author(s): Yoshifumi Okochi, Yasuaki Aratani, Kazuo Suzuki, Nana Miyawaki, Mari Sasaki, Yasushi Okamura
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 長野県松本文化会館
  • Year and Date: 2012-03-29
• [Presentation] Regulation of ROS production by VSOP is a voltage-gated proton channel that functions in phagocytes (2011)
  • Author(s): Yoshifumi Okochi, Tsukasa Kawahara、Yasushi Okamura
  • Organizer: 第88回日本生理学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-28
• [Presentation] 貧食細胞における電位依存性プロトンチャネルVSOPによる活性酸素産生制御の解析 (2011)
  • Author(s): 大河内善史、岡村康司
  • Organizer: 第88回日本生理学会大会
  • Place of Presentation: 神奈川(横浜)
  • Year and Date: 2011-03-28
• [Presentation] The role of VSOP is a voltage-gated proton channel that functions in phagocytes (2010)
  • Author(s): Yoshifumi Okochi, Tsukasa Kawahara and Yasushi Okamura
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-05-20
• [Remarks]
  • URL: http://www.med.osaka-u.ac.jp/pub/phys2/okamura/index.html
• [Remarks]
  • URL: http://www.med.osaka-u.ac.jp/pub/phys2/okamura/index.html
• [Remarks]
  • URL: http://www.med.osaka-u.ac.jp/pub/phys2/tougouseiri/menu.htm