Establishment of simultaneous real-time measurement system for myocardial mechanenergetics and calcium dynamics in rat whole hearts.

• Project/Area Number: 22790216
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General physiology
• Research Institution: Nara Medical University
• Principal Investigator: OBATA Koji 奈良県立医科大学, 医学部, 助教 (40378229)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
• Keywords: カルシウム / カルシウムイメージング / 心臓 / 心臓力学 / 興奮収縮連関 / 筋小胞体 / トランスジェニックラット / 酸素消費 / エネルギー代謝 / 筋収縮 / 遺伝子組換え型蛍光カルシウムプローブ

Research Abstract

A question of long-standing to cardiac physiologists is whether E-C coupling VO_2 will be really independent of preload. To answer the question, we tried to develop the simultaneous real-time measurement system for myocardial mechanoenergetics and calcium dynamics in the blood-perfused excised heart preparations using G-CaMP-expressed transgenic rats.

Research Products

• [Journal Article] Evaluation of left ventricular mechanical work and energetics of normal hearts in SERCA2a transgenic rats. (2012)
  • Author(s): Zhang GX, Obata K, Takaki M, 他8名
  • Journal Title: J Physiol Sci Volume: 62 Issue: 3 Pages: 221-231
  • DOI: 10.1007/s12576-012-0200-4
  • NAID: 10030742386
  • Peer Reviewed
• [Journal Article] NHE-1 blockade reversed changes in calcium transient in myocardial slices from isoproterenol-induced hypertrophied rat left ventricle (2012)
  • Author(s): Hattori H, Obata K, Takaki M, 他
  • Journal Title: Biochem Biophys Res Commun Volume: 419 Issue: 2 Pages: 431-435
  • DOI: 10.1016/j.bbrc.2012.02.041
  • Peer Reviewed
• [Journal Article] Comparison of effects of a selective 5-HT reuptake inhibitor versus a 5-HT4 receptor agonist on in vivo neurogenesis at the rectal anasotomosis in rats. (2012)
  • Author(s): Kawahara I. Obata K, Takaki M
  • Journal Title: Am J Physiol Gastrointest Liver Physiol Volume: 302 Issue: 6 Pages: 588-597
  • DOI: 10.1152/ajpgi.00284.2011
  • Peer Reviewed
• [Journal Article] NHE-1 participates in isoproterenol-induced down-regulation of SERCA2a and development of cardiac remodeling in rat hearts (2011)
  • Author(s): Shibata M, Obata K, Takaki M, 他
  • Journal Title: Am J Physiol Heart Circ Physiol Volume: 301 Issue: 5 Pages: 2154-2160
  • DOI: 10.1152/ajpheart.00483.2011
  • Peer Reviewed
• [Journal Article] Invitro enhanced differentiation of neural networksin ES gut-like organ from mouse ES cells by a 5-HT4-receptor activation. (2011)
  • Author(s): Takaki M, Misawa H, Matsuyoshi H, Kawahara I,Goto K, Zhang GX, Obata K, Kuniyasu H
  • Journal Title: Biochem Biophys Res Commun. Volume: 406 Issue: 4 Pages: 529-533
  • DOI: 10.1016/j.bbrc.2011.02.072
  • Peer Reviewed
• [Journal Article] Changes in contractile and electrical activity in the ileum of DSS-induced colitis model W/Wv mutant mice (2010)
  • Author(s): Matsuyoshi H, Nakagawa T, Zhang GX, Obata K, Misawa H, Kawahara I, Takaki M
  • Journal Title: Journal of Smooth Muscle Research Volume: 46 Issue: 3 Pages: 143-156
  • DOI: 10.1540/jsmr.46.143
  • NAID: 130000860969
  • ISSN: 0916-8737, 1884-8796
  • Peer Reviewed
• [Journal Article] Effects of Angiotensin Type I Receptor Blockade on the Cardiac Raf/MEK/ERK Cascade Activated via Adrenergic Receptors (2010)
  • Author(s): Zhang GX, Kimura S, Murao K, Yu X, Obata K, Matsuyoshi H, Takaki M
  • Journal Title: Journal of Pharmacological Sciences Volume: 113 Issue: 3 Pages: 224-233
  • DOI: 10.1254/jphs.09336FP
  • NAID: 10029889243
  • ISSN: 1347-8613, 1347-8648
  • Peer Reviewed
• [Journal Article] A cardioprotective agent of a novel calpain inhibitor,SNJ- 1945 exerts beta1-actions on left ventricular mechanical work and energetics. (2010)
  • Author(s): Yoshikawa Y, Obata K, Asada K, Takaki M, 他
  • Journal Title: Am J Physiol Heart Physiol. Volume: 299 Issue: 2 Pages: 396-401
  • DOI: 10.1152/ajpheart.00153.2010
  • Peer Reviewed
• [Journal Article] A cardioprotective agent of a novel calpain inhibitor, SNJ-1945 exerts beta1-actions on left ventricular mechanical work and energetics. (2010)
  • Author(s): Yoshikawa, et al.
  • Journal Title: Am J Physiol Heart Circ Physiol. Volume: 299(2) Pages: 396-401
  • Peer Reviewed
• [Journal Article] Ca(2+) channel blocker benidipine promotes coronary angiogenesis and reduces both left-ventricular diastolic stiffness and mortality in hypertensive rats. (2010)
  • Author(s): Nishizawa, et al.
  • Journal Title: J Hypertens. Volume: 28(7) Pages: 1515-1526
  • Peer Reviewed
• [Journal Article] Effects of AT1 Receptor Blockade on the Cardiac Raf/MEK/ERK Cascade Activated via Adrenergic Receptors. (2010)
  • Author(s): Zhang, et al.
  • Journal Title: J Pharmacol Sci. Volume: 113(3) Pages: 224-233
  • Peer Reviewed
• [Presentation] Suppressed Ca^<2+> handling and cross-bridge cycling underlie negative inotropism in hyperthermic rat hearts (2012)
  • Author(s): Obata K, Takeshita D, Mitsuyama S, Zhang G-X, Takaki M
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 長野県松本文化会館長野
• [Presentation] Na^+/H^+exchanger-1 blocker treatment reversed changes in calcium transient in myocardial slices from isoproterenol-induced hypertrophied rat left ventricles (2012)
  • Author(s): Takeshita D, Hattori H, Matsuoka S, Shibata M, Kimb B, Takeuchi A, Zhang GX, Obata K, Takaki M
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 長野県松本文化会館長野
• [Presentation] Left ventricular mechanoenergetics in isoproterenol-induced hypertrophied SERCA2a transgenic rats (2012)
  • Author(s): Mitsuyama S, Obata K, Takeshita D, Ito H, Takaki M
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 長野県松本文化会館長野
• [Presentation] 血液交叉灌流摘出ラット心臓において、高温条件下ではカルシウムハンドリングおよびクロスブリッジ機構の抑制によって収縮力が低下する (2012)
  • Author(s): 小畑孝二, その他
  • Organizer: 第89回日本生理学会
  • Place of Presentation: 松本(長野県松本文化会館)
• [Presentation] Na^+/H^+ exchanger-1阻害薬は、イソプロテレノール誘導肥大心の心筋スライスにおいてCa^<2+>トランジェントを正常化する (2012)
  • Author(s): 竹下大輔、小畑孝二, その他
  • Organizer: 第89回日本生理学会
  • Place of Presentation: 松本(長野県松本文化会館)
• [Presentation] SERCA2a過剰発現ラットにおけるイソプロテレノール持続投与による肥大心の左室力学的エネルギー学的性質 (2012)
  • Author(s): 光山晋一、小畑孝二, その他
  • Organizer: 第89回日本生理学会
  • Place of Presentation: 松本(長野県松本文化会館)
• [Presentation] 血液交叉灌流摘出ラット心臓の高温条件下におけるメカノエナジェティクスの研究 (2011)
  • Author(s): 小畑孝二, 竹下大輔, 光山晋一, 伊藤治男, 高木都
  • Organizer: 第21回日本病態生理学会
  • Place of Presentation: 日本大学医学部東京
• [Presentation] Symposium 45 New approach of analysis for cardiac function using cross-circulation system with blood Cardiac mechanoenergetics in SERCA2a transgenic rats (2011)
  • Author(s): Zhang G-X, Obata K, Takaki M
  • Organizer: 第88回日本生理学会大会・第116回日本解剖学会総会・全国学術集会合同大会
  • Place of Presentation: パシフィコ横浜会議センター横浜
• [Presentation] A novel NHE-1 inhibitor ameliorates cardiac Ca^<2+> handling impairment and prevents the development of cardiac remodeling in isoproterenol-infused rats (2011)
  • Author(s): Takeshita D, Zhang GX, Obata K, Misawa H, Matsuyoshi H, Takaki M
  • Organizer: 第88回日本生理学会大会・第116回日本解剖学会総会・全国学術集会合同大会
  • Place of Presentation: パシフィコ横浜会議センター横浜
• [Presentation] 血液交叉灌流摘出ラット心臓の高温条件下におけるメカノエナジェティクスの研究 (2011)
  • Author(s): 小畑孝二, その他
  • Organizer: 第21回日本病態生理学会
  • Place of Presentation: 東京(日本大学医学部)
• [Presentation] ラット急性虚血モデルにおけるカルパイン阻害薬の作用-1心拍あたりの総機械エネルギー(PVA)解析から- (2010)
  • Author(s): 田中みどり, 張国興, 小畑孝二, 松吉ひろ子, 吉川義朗, 竹下大輔, 三澤裕美, 谷口繁樹, 高木都
  • Organizer: 第103回近畿生理学談話会
  • Place of Presentation: 大阪大学銀杏会館
  • Year and Date: 2010-10-02
• [Presentation] Na^+/H^+交換体阻害薬経口投与によるイソプロテレノール誘導性肥大心の肥大抑制作用とCa^<2+>トランジェントへの影響 (2010)
  • Author(s): 竹下大輔, 柴田宗孝, 服部宇孜, 張国興, 小畑孝二, 松吉ひろ子, 三澤裕美, 高木都
  • Organizer: 第103回近畿生理学談話会
  • Place of Presentation: 大阪大学銀杏会館
  • Year and Date: 2010-10-02
• [Presentation] 新しいカルパイン阻害剤SNJ-1945添加心筋保護液の虚血再灌流障害防止効果:ラット血液交叉灌流摘出心臓標本を用いて (2010)
  • Author(s): 吉川義朗, 張国興, 小畑孝二, 大賀好美, 松吉ひろ子, 谷口繁樹, 高木都
  • Organizer: 第31回日本循環制御医学会総会
  • Place of Presentation: 千里ライフサイエンスセンター
• [Presentation] イソプロテレノール誘導性肥大心におけるCa^<2+>トランジェントと経口Na+/H+交換体(NHE-1)阻害薬の肥大抑制作用 (2010)
  • Author(s): 柴田宗孝, 張国興, 小畑孝二, 竹下大輔, 三澤裕美, 松吉ひろ子, 高木都
  • Organizer: 第31回日本循環制御医学会総会
  • Place of Presentation: 千里ライフサイエンスセンター
• [Presentation] An experimental study in transgenic rat models. (2010)
  • Author(s): Obata K
  • Organizer: BBSRC Japan Partnering Programme 2007-2011.CARDIAC ELECTRO-MECHANICAL FUNCTION Cell-Organ Cross-Talk Revealed via Integration of Experiments and Models.
  • Place of Presentation: Oxford.UK
• [Remarks] 奈良県立医科大学生理学第2講座HP
• [Remarks]
  • URL: http://www.naramed-u.ac.jp/~2phy/