| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Research Abstract |
It was previously reported that constant exposure to moderate heat facilitates progenitor cell proliferation and neuronal differentiation in the hypothalamus of rats. The present study has investigated localization and responsiveness to heat of hypothalamic newborn neurons. Male Wistar rats, 5 weeks old, were subjected to an ambient temperature(T_a) of 32°C for 40 days, while control rats were constantly kept at a T_a of 24°C. Bromodeoxyuridine(BrdU) was intraperitoneally injected daily for 5 consecutive days(50 mg/kg/day) after commencing heat exposure. After the end of heat exposure period, all rats were kept at a T_a of 24°C for 48 h, and subjected to heat tolerance test, i. e. they were exposed a T_a of 36°C for 3 h. After the test, brain was removed for immunohistochemical analysis. According to our estimate in HE53, 23. 2% of BrdU+/NeuN+cells were located in the POA/AH, while 32. 9% were detected in the VMH, DMH or PVH. Moreover, mitosis inhibitor, Cytosine-・-arabinoside, attenuated the intensity of heat tolerance in heat-acclimated rats. Changes of thermoregulatory function in long-term heat-acclimated rats may possibly be in part attributable to generations of new neurons in the hypothalamus.
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