| Project/Area Number |
22790228
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | National Institute for Environmental Studies |
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Principal Investigator |
MAEKAWA Fumihiko 独立行政法人国立環境研究所, 環境健康研究センター, 主任研究員 (40382866)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 摂食調節 / 全身代謝調節 / 神経エネルギー学 / 弓状核 / 摂食 / ニューロペプチドY / モノカルボン酸 / エネルギー感知 |
|---|
| Research Abstract |
To clarify whether the nutrients taken up from extracellular space and the consequent energy derived from the nutrients affect the physiological function and cellular morphology of NPY neuron, we examined the effects of arsenic, an inhibitor of glycolysis, on cellular survival, neurite outgrowth and glutamate AMPA receptor expression in primary culture of NPY neurons. Both significant suppressions of total neurite length and celluar survival were found at the concentration above 2μM. Immunofluorescence staining of glutamate receptors in NPY-hrGFP neuron revealed that arsenic above 2μM decreased a subtype of glutamate AMPA receptor expression. On the other hand, the enhancements of cellular survival and an AMPA receptor expression were found at the concentration below 0.5μM. These results demonstrate the possibility that neuronal activity is dramatically changeable by the nutrients transported and consequent intracellular energy level in NPY neurons.
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