| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Research Abstract |
This research was planned to characterize extra-nuclear estrogen receptors that mediate non-genomic effects of estrogens in the central nervous system. The genomic organization of human, mouse, and rat estrogen receptor α (ERα) genes was re-examined. The analysis revealed more complicated genomic structures of the ERα genes and localization of novel promoters and exons in the 5’-, intronic and 3’-regions of the genes. Furthermore, the analysis demonstrated that alternative promoter usage and alternative splicing generated numerous mRNA variants that encoded N-terminally- and C-terminally-truncated estrogen receptors. The variant proteins exhibited distinct functional properties and cellular localization compared with those of the wild-type ERα proteins.
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