Analysis of input signal specific to mammalian circadian center

• Project/Area Number: 22790234
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Environmental physiology (including Physical medicine and Nutritional physiology)
• Research Institution: Kinki University
• Principal Investigator: KOINUMA Satoshi 近畿大学, 医学部, 助教 (10340770)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 生物時計 / 視交叉上核 / cAMP / Forskolin / 位相反応曲線 / VIP / Vipr2 / Per2

Research Abstract

In order to investigate signals transmitted in the suprachiasmatic nucleus(SCN), perturbation of cAMP signal by chronic administration of Forskolin(FK) to Per2 :: Luc rat SCN slice was performed. Administration of FK divided the SCN into two regions based on the period length. One of which showed shorter period than 24 h(SPR) and another showed longer than 24 h(LPR). SPR was localized in the medial region of the dorsal SCN and LPR localized in the lateral region, respectively. Intriguingly, the SPR and a region where phase wave starts were overlapped. These results suggest that cAMP dependent signal is involved in the synchronization of the SCN subregions. Further study of SPR may lead to the understanding of unknown mechanisms and roles of the phase wave in the mammalian circadian center.

Research Products

• [Journal Article] Differential entrainment of peripheral clocks in the rat by glucocorticoid and feeding (2012)
  • Author(s): Sujino, M., Furukawa, K., Koinuma, S., Fujioka, A., Nagano, M., Iigo, M., Shigeyoshi, Y
  • Journal Title: Endocrinology Volume: 153 Pages: 2277-2286
  • Peer Reviewed
• [Journal Article] Development of the circadian oscillator during differentiation of mouse embryonic stem cells in vitro (2010)
  • Author(s): Yagita, K., Horie, K., Koinuma, S., Nakamura, W., Yamanaka, I., Urasaki, A., Shigeyoshi, Y., Kawakami, K., Shimada, S., Takeda, J. and Uchiyama, Y
  • Journal Title: Proc Natl Sci USA Volume: 107 Pages: 3846-3851
  • Peer Reviewed
• [Presentation] 哺乳類体内時計中枢における領域的周期解析 (2011)
  • Author(s): 鯉沼聡, 他
  • Organizer: 第87回日本解剖学会近畿支部学術集会
  • Place of Presentation: 兵庫県西宮市(兵庫医科大学西宮キャンパス)
  • Year and Date: 2011-12-03
• [Presentation] 哺乳類体内時計中枢における領域的周期解析 (2011)
  • Author(s): 鯉沼聡
  • Organizer: 第87回日本解剖学会近畿支部学術集会
  • Place of Presentation: 兵庫医科大学西宮キャンパス(兵庫県西宮市)
  • Year and Date: 2011-12-03
• [Presentation] Mathematical model of mammalian circadian center as a many-body system of the limit cycle oscillators (2011)
  • Author(s): Asakawa, T., 他
  • Organizer: European Conference on Mathematical and Theoretical Biology-ECMTB 2011-
  • Place of Presentation: Krakow, Poland
• [Presentation] The underlying mechanism that generate phase waves in the mammalian circadian center (2011)
  • Author(s): Shigeyoshi, Y., 他
  • Organizer: The 3rd International conference on cognitive neurodynamics
  • Place of Presentation: 北海道(Hilton Niseko Village)
• [Presentation] REGIONAL PERIOD DIFFERENCE THAT GENERATES A PHASE GRADIENT IN THE MAMMALIAN CIRCADIAN CENTER (2011)
  • Author(s): Shigeyoshi, Y., 他
  • Organizer: 3rd World congress of chronobiology
  • Place of Presentation: Puebla, Mexico
• [Presentation] 視交叉上核組織切片培養における細胞間同期機構の解析 (2010)
  • Author(s): 鯉沼聡, 他
  • Organizer: 第17回日本時間生物学会学術大会
  • Place of Presentation: 東京都新宿区(早稲田大学国際会議場)
  • Year and Date: 2010-11-21
• [Presentation] グルココルチコイド刺激による肝腎末梢時計の位相変化の違い (2010)
  • Author(s): 筋野貢
  • Organizer: 第17回日本時間生物学会学術大会
  • Place of Presentation: 東京都新宿区(早稲田大学国際会議場)
  • Year and Date: 2010-11-21
• [Presentation] 視交叉上核における領域間同期機構の探索-ペプチド産生細胞の働き- (2010)
  • Author(s): 長野護, 他
  • Organizer: 第17回日本時間生物学会学術大会
  • Place of Presentation: 東京都新宿区(早稲田大学国際会議場)
  • Year and Date: 2010-11-21
• [Presentation] 視交叉上核組織切片培養における細胞間同期機構の解析 (2010)
  • Author(s): 鯉沼聡
  • Organizer: 第17回日本時間生物学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2010-11-21
• [Presentation] Mathematical model of suprachiasmatic nucleus to describe the asymmetric resynchronization after an abrupt shift of the light : dark cycle (2010)
  • Author(s): Asakawa, T., 他
  • Organizer: Neuroscience 2010
  • Place of Presentation: San Diego
• [Presentation] A mechanism coupling two major subregions of the mammalian central circadian pacemaker (2010)
  • Author(s): Shigeyoshi, Y., 他
  • Organizer: Neuroscience 2010
  • Place of Presentation: San Diego