| Project/Area Number |
22790240
|
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
|---|
| Research Field |
General pharmacology
|
|---|
| Research Institution | Asahikawa Medical College |
|---|
Principal Investigator |
SEMBA Shingo 旭川医科大学, 医学部, 助教 (40466496)
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
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| Keywords | シグナル伝達 / 血管平滑筋 / 一酸化窒素 / ミオシンホスファターゼ / MYPT1 / MYPT |
|---|
| Research Abstract |
In this study, we utilized affinity chromatography technique using MYPT1 fragment that was thiophosphorylated at Thr-696 (tp-MYPT) as ligand for isolation of MYPT1-phosphatases. By peptide mass fingerprinting analysis, we identified PP1cδ as one of the proteins that specifically bound to tp-MYPT but not unphosphorylated (unp)-MYPT Sepharose resin. Furthermore, overexpression of PP1cδ in smooth muscle cell line resulted the inhibition of the MYPT1 phosphorylation at Thr-696 induced by endothelin-1 sitimulation. Our results indicate that PP1cδ facilitates the dis-inhibition of MLCP activity by auto-dephosphorylating MYPT1.
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