Reveal of basospasums signal transduction with sphingosyl phosphorylcholine And the inhibitory effects of blebbistatin

• Project/Area Number: 22790242
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General pharmacology
• Research Institution: Gunma University
• Principal Investigator: TANAKA Hideyuki 群馬大学, 大学院・保健学研究科, 助教 (70343085)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: Sphingosyl phosphorylcholine (SPC) / マグナポディア / 共焦点レーザー顕微鏡 / Blebbistatin / 血管攣縮性動脈硬化 / 電子顕微鏡 / Sphingosyl phosphorylcholine / Blebbistatinn

Research Abstract

A part of intracellular signal transduction was revealed with sphingosyl phosphorylcholine(SPC) of smooth muscle cell. And it was suggested that Blebbistatin inhibited the basospasms with SPC.

Research Products

• [Journal Article] Myosin Light Chain Kinase/Actin Interaction in Phorbol Dibutyrate Stimulated Smooth Muscle Cells (2011)
  • Author(s): Sean E. Thatcher, Mike E. Fultz, Hideyuki Tanaka, Haruo Hagiwara, Hou-Li Zhang, Kohichi Hayakawa, Shingji Yoshiyama, Akio Nakamura, Hong-Hui Wang, Takeshi Katayama, Masaru Watanabe, Yuan Lin, Gray L. Wright, Kazuhiro Kohama
  • Journal Title: Journal of Pharmacological Society Volume: Vol.116,No1 Pages: 116-127
  • NAID: 10029894196
  • Peer Reviewed
• [Journal Article] Transport of single cells using an actin bundle-myosin bionanomotor transport system (2011)
  • Author(s): Hideyo Takatsuki, Hideyuki Tanaka, Kevin M Rice, Madhukar B Kolli, Siva K Nalabotu, Kazuhiro Kohama, Parviz Famouri, Eric R Blough
  • Journal Title: Nanotechnology Volume: Vol.22 Pages: 245101-245101
  • Peer Reviewed
• [Journal Article] A galactosamine-mediated drug delivery carrier for targeted liver cancer therapy (2011)
  • Author(s): Zheyu Shen, Wei Wei, Hideyuki Tanaka, Kazuhiro Kohama, Guanghui Ma, Toshiki Dogashi, Yasuyuki Maki, Hong-Hui Wang, Jing-Xiu Bi, Sheng Dai
  • Journal Title: Pharmacological Research Volume: Vol,64 Pages: 410-419
  • Peer Reviewed
• [Journal Article] Transport of single cells using an actin bundle-myosin bionanomotor transport system (2011)
  • Author(s): 高附英世
  • Journal Title: Nanotechnology Volume: 22 Issue: 24 Pages: 245101-245101
  • DOI: 10.1088/0957-4484/22/24/245101
  • Peer Reviewed
• [Journal Article] A galactosamine-mediated drug delivery carrier for targeted liver cancer therapy (2011)
  • Author(s): Zheyu Shen
  • Journal Title: Pharmacological Research Volume: 64 Pages: 410-419
  • Peer Reviewed
• [Journal Article] Myosin Light Chain Kinase/Actin Interaction in Phorbol Dibutyrate Stimulated Smooth Muscle Cells (2011)
  • Author(s): Sean E.Thatcher
  • Journal Title: Journal of Pharmacological Society Volume: 116(1) Pages: 116-127
  • Peer Reviewed
• [Presentation] Morphological analysis of effects with arachidonic acid of smooth muscle cells (2012)
  • Author(s): Hideyuki Tanaka, Haruo Hagiwara
  • Organizer: 第117回日本解剖学会総会・学術集会
  • Place of Presentation: 山梨大学(甲府)
  • Year and Date: 2012-03-27
• [Presentation] スフィンゴ脂質(SPC)の作用による平滑筋細胞内アクチンの局在変化 (2011)
  • Author(s): 田中秀幸, 萩原治夫
  • Organizer: 第43回日本臨床分子形態学会総会・学術集会
  • Place of Presentation: 大阪医科大学(高槻市)
  • Year and Date: 2011-09-09
• [Presentation] Cytoskeletal effect of sphingosyl lipid (SPC) in smooth muscle cells (2011)
  • Author(s): Hideyuki Tanaka, Haruo Hagiwara
  • Organizer: 第116回日本解剖学会総会・学術集会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2011-03-30
• [Presentation] Cytoskeletal effects of myosin inhibitors in muscle cells (2011)
  • Author(s): Hideyuki Tanaka, Masaru Watanabe, Takeshi Katayama, Kazuhiro Kohama
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2011-03-22
• [Presentation] ラット結腸平滑筋形質膜におけるデンスプラーク領域とカベオラ密集領域 (2010)
  • Author(s): 田中秀幸, 萩原治夫
  • Organizer: 第42回日本臨床分子形態学会総会・学術集会
  • Place of Presentation: 東レ総合研修センター(三島)
  • Year and Date: 2010-09-24