| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Research Abstract |
The uptake of OxLDL by macrophages in atherosclerotic lesions is mediated by so-called scavenger receptors. The uptake is further enhanced by plasmin activities. There are 4 SRs in macrophages, which are Msr1, Cd36, Orl1, and Cxcl16. It is still unknown that what SRs are enhanced for OxLDL uptake by plasmin activities. In this study, several cell lines were utilized for exogenous expressions of these SRs, however, some of SRs were difficult to be expressed. Therefore, macrophages were directly collected from Ldlr-/-/Apobec1-/- mice. At the same time, RAW264 cell line, which is murine macrophages, is tested for the expression of these 4 SRs. The cell line expresses 4 SRs, therefore, we are now destroying these SRs from the cell line to test plasmin-mediated OxLDL uptake.
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