The role of plasmin activity for foam cell formation of macrophages under hypercholesterolemia
| Project/Area Number |
22790247
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General pharmacology
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | plasminogen / scavenger / OxLDL / atherosclerosis / Plasminogen / Scavenger / Atherosclerosis |
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| Research Abstract |
The uptake of OxLDL by macrophages in atherosclerotic lesions is mediated by so-called scavenger receptors. The uptake is further enhanced by plasmin activities. There are 4 SRs in macrophages, which are Msr1, Cd36, Orl1, and Cxcl16. It is still unknown that what SRs are enhanced for OxLDL uptake by plasmin activities. In this study, several cell lines were utilized for exogenous expressions of these SRs, however, some of SRs were difficult to be expressed. Therefore, macrophages were directly collected from Ldlr-/-/Apobec1-/- mice. At the same time, RAW264 cell line, which is murine macrophages, is tested for the expression of these 4 SRs. The cell line expresses 4 SRs, therefore, we are now destroying these SRs from the cell line to test plasmin-mediated OxLDL uptake.
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Report
(5 results)
Research Products
(26 results)
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[Journal Article] Life-threatening hemorrhage and prolonged wound healing are remarkable phenotypes manifested by complete plasminogen activator inhibitor-1 deficiency in humans2011
Author(s)
T Iwaki, A Tanaka, Y Miyawaki, A Suzuki, T Kobayashi, J Takamatsu, T Matsushita, K Umemura, T Urano, T Kojima, T Terao, N Kanayama
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Journal Title
J Thromb Haemost
Volume: 9(6)
Issue: 6
Pages: 1200-1206
DOI
Related Report
Peer Reviewed
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