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The role of plasmin activity for foam cell formation of macrophages under hypercholesterolemia

Research Project

Project/Area Number 22790247
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field General pharmacology
Research InstitutionHamamatsu University School of Medicine

Principal Investigator

IWAKI TAKAYUKI  浜松医科大学, 医学部, 准教授 (70509463)

Project Period (FY) 2010-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywordsplasminogen / scavenger / OxLDL / atherosclerosis / Plasminogen / Scavenger / Atherosclerosis
Research Abstract

The uptake of OxLDL by macrophages in atherosclerotic lesions is mediated by so-called scavenger receptors. The uptake is further enhanced by plasmin activities. There are 4 SRs in macrophages, which are Msr1, Cd36, Orl1, and Cxcl16. It is still unknown that what SRs are enhanced for OxLDL uptake by plasmin activities. In this study, several cell lines were utilized for exogenous expressions of these SRs, however, some of SRs were difficult to be expressed. Therefore, macrophages were directly collected from Ldlr-/-/Apobec1-/- mice. At the same time, RAW264 cell line, which is murine macrophages, is tested for the expression of these 4 SRs. The cell line expresses 4 SRs, therefore, we are now destroying these SRs from the cell line to test plasmin-mediated OxLDL uptake.

Report

(5 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (26 results)

All 2014 2013 2012 2011 2010 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (18 results) (of which Invited: 3 results)

  • [Journal Article] PAI-1, progress in understanding the clinical problem and its aetiology2012

    • Author(s)
      Iwaki T, Urano T, Umemura K
    • Journal Title

      British Journal of Haematology

      Volume: (In press) Issue: 3 Pages: 291-8

    • DOI

      10.1111/j.1365-2141.2012.09074.x

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fusion of fluorescent protein to puromycin N-acetyltransferase is useful in Drosophila Schneider S2 cells expressing heterologous proteins2012

    • Author(s)
      Nagahashi K, Umemura K, Kanayama N, Iwaki T.
    • Journal Title

      Cytotechnology

      Volume: 65(2) Issue: 2 Pages: 173-8

    • DOI

      10.1007/s10616-012-9473-y

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The first report of uncontrollable subchorionic and retroplacental haemorrhage inducing preterm labour in complete PAI-1 deficiency in a human2012

    • Author(s)
      Iwaki T, T Nagahashi K, Kobayashi T, Umemura K, Terao T, Kanayama N
    • Journal Title

      Thromb Res

      Volume: 129

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Life-threatening hemorrhage and prolonged wound healing are remarkable phenotypes manifested by complete plasminogen activator inhibitor-1 deficiency in humans2011

    • Author(s)
      T Iwaki, A Tanaka, Y Miyawaki, A Suzuki, T Kobayashi, J Takamatsu, T Matsushita, K Umemura, T Urano, T Kojima, T Terao, N Kanayama
    • Journal Title

      J Thromb Haemost

      Volume: 9(6) Issue: 6 Pages: 1200-1206

    • DOI

      10.1111/j.1538-7836.2011.04288.x

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A single plasmid transfection that offers a significant advantage associated with puromycin selection, fluorescence-assisted cell sorting, and doxycycline-inducible protein expression in mammalian cells2011

    • Author(s)
      Iwaki T, Umemura K.
    • Journal Title

      Cytotechnology

      Volume: 63 Pages: 337-43

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The first report of uncontrollable subchorionic and retroplacental haemorrhage inducing preterm labour in complete PAI-1 deficiency in a human2011

    • Author(s)
      Iwaki T, Nagahashi K, Kobayashi T, Umemura K, Terao T, Kanayama N.
    • Journal Title

      Thromb Res.

      Volume: 129

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The generation and characterization of mice expressing a plasmin-inactivating active site mutation2010

    • Author(s)
      Iwaki T, Malinverno C, Smith D, Xu Z, Liang Z, Ploplis VA, Castellino FJ.
    • Journal Title

      J Thromb Haemost

      Volume: 8 Pages: 2341-4

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Life-threatening haemorrhage and prolonged wound healing are remarkable phenotypes manifested by complete PAI-1 deficiency in humans

    • Author(s)
      Takayuki Iwaki, et al
    • Journal Title

      Journal of Thrombosis and Haemostasis

      Volume: (In press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] PAI-1 deficiency in humans and mice2014

    • Author(s)
      岩城孝行
    • Organizer
      日本検査血液学会静岡支部総会
    • Place of Presentation
      静岡
    • Year and Date
      2014-03-15
    • Related Report
      2013 Final Research Report
  • [Presentation] Analysis of a new PAI-1 deficient patient2014

    • Author(s)
      岩城孝行
    • Organizer
      第87回日本薬理学会
    • Place of Presentation
      仙台
    • Related Report
      2013 Final Research Report
  • [Presentation] Analysis of a new PAI-1 deficient patient2014

    • Author(s)
      岩城 孝行
    • Organizer
      第87回日本薬理学会年会
    • Place of Presentation
      仙台
    • Related Report
      2013 Annual Research Report
  • [Presentation] A Plasminogen Activator Inhibitor-1 Deficiency Reduces the Progression of Atherosclerosis in a Murine Model of Human Familial Hypercholesterolemia2013

    • Author(s)
      岩城孝行
    • Organizer
      第86回日本薬理学会
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
  • [Presentation] PAI-1 deficiency Men and Mice2013

    • Author(s)
      Takayuki Iwaki
    • Organizer
      VIII Aso International Meeting
    • Place of Presentation
      Aso
    • Related Report
      2013 Final Research Report
  • [Presentation] PAI-1 deficiency2013

    • Author(s)
      岩城孝行
    • Organizer
      日本血栓止血学会
    • Place of Presentation
      山形
    • Related Report
      2013 Final Research Report
  • [Presentation] PAI-1 deficiency Men and Mice2013

    • Author(s)
      Takayuki Iwaki
    • Organizer
      The 8th Aso International Meeting
    • Place of Presentation
      阿蘇
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] PAI-1 欠損症2013

    • Author(s)
      岩城 孝行
    • Organizer
      第35回日本血栓止血学会学術集会
    • Place of Presentation
      山形
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Is the phenotype manifested by complete PAI-1 deficiency in human compatible to that in mouse?2013

    • Author(s)
      Kotomi Nagahashi, Takayuki Iwaki, Katsuhiro Takano, Yukio Ozaki, Naohiro Kanayama, Kazuo Uemura, Tetsumei Urano
    • Organizer
      The XIVth International Workshop
    • Place of Presentation
      米国、ノートルダム
    • Related Report
      2013 Annual Research Report
  • [Presentation] A Plasminogen Activator Inhibitor-1 Deficiency Reduces the Progression of Atherosclerosis in a Murine Model of Human Familial Hypercholesterolemia2013

    • Author(s)
      Takayuki Iwaki
    • Organizer
      第86回日本薬理学会
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] Is the phenotype manifested by complete PAI-1 deficiency in human compatible to that in mouse?2012

    • Author(s)
      岩城孝行, 梅村和夫
    • Organizer
      日本薬理学会
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report 2011 Annual Research Report
  • [Presentation] The Role of Blood Coagulation and Fibrinolytic Factors in the Placenta2012

    • Author(s)
      Takayuki Iwaki
    • Organizer
      XVIII IFPA Meeting
    • Place of Presentation
      Hiroshima
    • Related Report
      2013 Final Research Report
  • [Presentation] The Role of Blood Coagulation and Fibrinolytic Factors in the Placenta2012

    • Author(s)
      Takayuki Iwaki
    • Organizer
      18th IFPA Meeting 2012 in Hiroshima
    • Place of Presentation
      Hiroshima, Japan
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] PAI-1 deficiency in humans and mice2011

    • Author(s)
      Takayuki Iwaki, Takao Kobayashi, Kazuo Umemura, Tetsumei Urano, Tetsuhito Kojima, Naohiro Kanayama
    • Organizer
      XXIII ISTH Congress & 57th Annual SSC Meeting
    • Place of Presentation
      Kyoto
    • Related Report
      2013 Final Research Report
  • [Presentation] PAI-1 deficiency in humans and mice2011

    • Author(s)
      Iwaki T, Kobayashi T, Umemura K, Urano T, Kojima T, Kanayama N
    • Organizer
      XXIII Congress of the International Society on Thrombosis and Haemostasis
    • Place of Presentation
      Kyoto
    • Related Report
      2011 Annual Research Report
  • [Presentation] Plasminogen and Atherosclerosis in a Murine Model of Human Familial Hypercholesterolemia2010

    • Author(s)
      岩城 孝行
    • Organizer
      第8回血管オルビス
    • Place of Presentation
      東京
    • Year and Date
      2010-08-21
    • Related Report
      2013 Final Research Report 2010 Annual Research Report
  • [Presentation] A Plasminogen Deficiency Attenuates Atherosclerosis2010

    • Author(s)
      岩城孝行、梅村和夫
    • Organizer
      第33回日本血栓止血学会学術集会
    • Place of Presentation
      鹿児島
    • Year and Date
      2010-04-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] A Plasminogen Deficiency Attenuates Atherosclerosi2010

    • Author(s)
      岩城 孝行, 梅村 和夫
    • Organizer
      第33回日本血栓止血学会学術集会
    • Place of Presentation
      鹿児島
    • Related Report
      2013 Final Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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