Study on the function of a novel Wnt-signaling regulator PDZRN3 and the development of bone regeneration therapy

• Project/Area Number: 22790252
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General pharmacology
• Research Institution: Yamaguchi University
• Principal Investigator: HONDA Takeshi 山口大学, 大学院・医学系研究科, 助教 (30457311)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 間葉系幹細胞 / 骨芽細胞 / PDZRN3 / Wntシグナル

Research Abstract

Wnt-β-catenin signaling is a major pathway promoting osteoblastic differentiation. However, the exact mechanisms and molecular details regulating Wnt signaling still remain to be elucidated. We found that PDZRN3 is a novel regulator of Wnt-β-catenin signaling in the differentiation of C2C12 mouse mesenchymal progenitor cells into osteoblasts. PDZRN3 suppressed the protein level of LRP6, Wnt receptor, in which PDZRN3 binds to the activated(phosphorylated) LRP6. We proposed that PDZRN3 plays an important role in negative feedback control of BMP-2-induced osteoblast differentiation in C2C12 cells through inhibition of Wnt?-catenin signaling.

Research Products

• [Journal Article] PDZRN3 negatively regulates BMP-2 induced osteoblast differentiation through inhibition of Wnt signaling (2010)
  • Author(s): Honda T, Yamamoto H, Ishii A, Inui M
  • Journal Title: Mol. Biol. Cell Volume: 21 Pages: 3269-3277
  • Peer Reviewed
• [Journal Article] PDZRN3 negatively regulates BMP-2-induced osteoblast differentiation through inhibition of Wnt signaling. (2010)
  • Author(s): Honda T, Yamamoto H, Ishii A, Inui M.
  • Journal Title: Molecular Biology of the Cell. Volume: 21 Pages: 3269-3277
  • Peer Reviewed
• [Presentation] Regulation of adipocyte differentiation by PDZRN3 protein (2012)
  • Author(s): Takeshi Honda, Ishii Aiko, Makoto Inui
  • Organizer: 第85回日本薬理学会年会
  • Place of Presentation: 京都市国立京都国際会館
  • Year and Date: 2012-03-16
• [Presentation] PDZRN3蛋白質による脂肪細胞分化の制御機構 (2012)
  • Author(s): 本田健、石井愛子、乾誠
  • Organizer: 第85回日本薬理学会
  • Place of Presentation: 国立京都国際会館(京都市)
  • Year and Date: 2012-03-16
• [Presentation] 脂肪細胞分化におけるPDZRN3の役割 (2012)
  • Author(s): 石井愛子、本田健、乾誠
  • Organizer: 第117回山口大学医学会
  • Place of Presentation: 宇部市、山口大学霜仁会館
  • Year and Date: 2012-02-18
• [Presentation] 脂肪細胞分化におけるPDZRN3の役割 (2011)
  • Author(s): 石井愛子、本田健、乾誠
  • Organizer: 第64回日本薬理学会西南部会
  • Place of Presentation: 福岡市KKRホテル博多
  • Year and Date: 2011-11-20
• [Presentation] 脂肪細胞分化におけるPDZRN3の役割 (2011)
  • Author(s): 石井愛子、本田健、乾誠
  • Organizer: 第64回日本薬理学会西南部会
  • Place of Presentation: KKRホテル博多(福岡市)
  • Year and Date: 2011-11-20
• [Presentation] Negative regulation of Wnt signaling by PDZRN3 protein in osteoblastic differentiation (2011)
  • Author(s): 本田健、石井愛子、山本久斗、乾誠
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: 神奈川県(パシフィコ横浜)
  • Year and Date: 2011-03-23
• [Presentation] Negative regulation of Wnt signaling by PDZRN3 protein in osteoblastic differentiation (2011)
  • Author(s): Takeshi Honda, Ishii Aiko, Hisato Yamamoto, Makoto Inui
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: 横浜市パシフィコ横浜*東日本大震災により開催中止
• [Presentation] Wntシグナルを介したPDZRN3による骨芽細胞分化の制御 (2010)
  • Author(s): 本田健、石井愛子、山本久人、乾誠
  • Organizer: 第63回日本薬理学会西南部会
  • Place of Presentation: 鹿児島市かごしま県民交流センター
  • Year and Date: 2010-11-26
• [Presentation] Wntシグナルを介したPDZRN3による骨芽細胞分化の制御 (2010)
  • Author(s): 本田健、石井愛子、山本久斗、乾誠
  • Organizer: 第63回 日本薬理学会西南部会
  • Place of Presentation: 鹿児島県(鹿児島県民交流センター)
  • Year and Date: 2010-11-26