Pathophysiological relationship ofmitochondria to neuronal death during brain ischemia

• Project/Area Number: 22790256
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General pharmacology
• Research Institution: Kagoshima University
• Principal Investigator: KANBE Yuki 鹿児島大学, 大学院・医歯学総合研究科, 助教 (60549913)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 神経細胞死 / 細胞内情報伝達 / ミトコンドリア / 脳神経疾患 / シグナル伝達 / 脳・神経疾患

Research Abstract

Pituitary adenylate cyclase-activating polypeptide (PACAP) increases neurite outgrowth in many kind of neuronal cells, but signaling via its receptor PACAP-specific receptor has not been fully characterized. Because mitochondria also play an important role in neurodevelopment including dendritic branching, axonal guidanceand neurite outgrowth, we examined whether mitochondria contribute to PACAP-mediated neurite outgrowth. Present data suggest that mitochondrial activation plays a key role in PACAP-induced neurite outgrowth via a signaling pathway that includes PAC1R, PKA, andPgc1α.

Research Products

• [Journal Article] N-arachidonoyl glycine induces macrophage apoptosis via GPR18 (2012)
  • Author(s): Takenouchi R, Inoue K, Kambe Y, Miyata A
  • Journal Title: Biochem Biophys Res Commun Volume: 418(2) Pages: 366-371
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/22266325
  • Peer Reviewed
• [Journal Article] Role of Mitochondrial Activation in PACAP Dependent Neurite Outgrowth (2012)
  • Author(s): Kambe Y, Miyata A
  • Journal Title: J Mol Neurosci Volume: 48(3) Pages: 550-557
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/22460784
  • Peer Reviewed
• [Journal Article] Possible involvement of mitochondrial uncoupling protein-2 in cytotoxicity mediated by acquired N-methyl-d-aspartate receptor channels (2012)
  • Author(s): Fukumori R, Takarada T, Kambe Y, Nakazato R, Fujikawa K, Yoneda Y
  • Journal Title: Neurochem Int. Volume: 61 Pages: 498-505
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/22490607
  • Peer Reviewed
• [Journal Article] Regulatory mechanism of PAC1 gene expression via Sp1 by nerve growth factor in PC12 cells (2012)
  • Author(s): Miura A, Odahara N, Tominaga A, Inoue K, Kambe Y, Kurihara T, Miyata A
  • Journal Title: FEBS Lett Volume: 586 Pages: 1731-1735
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/22609358
  • Peer Reviewed
• [Journal Article] Possible involvement of mitochondrial uncoupling protein-2 in cytotoxicity mediated by acquired N-methyl-d-aspartate receptor channels (2012)
  • Author(s): Fukumori, R.
  • Journal Title: Neurochem Int Volume: 61 Pages: 498-505
  • Peer Reviewed
• [Journal Article] Regulatory mechanism of PAC1 gene expression via Sp1 by nerve growth factor in PC12 cells (2012)
  • Author(s): Miura, A.
  • Journal Title: FEBS Lett Volume: 586 Pages: 1731-5
  • Peer Reviewed
• [Journal Article] A possible pivotal role of mitochondrial free calcium in neurotoxicity mediated by N-methyl-d-aspartate receptors in cultured rat hippocampal neurons (2011)
  • Author(s): Kambe Y, Nakamichi N, Takarada T, Fukumori R, Nakazato R, Hinoi E, Yoneda Y
  • Journal Title: Neurochem Int Volume: 59(1) Pages: 10-20
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/21669242
  • Peer Reviewed
• [Presentation] C-TYPE NATRIURETIC PEPTIDE MODULATES THE PERMEABILITY OF BLOOD-BRAIN BARRIER (2013)
  • Author(s): Manoj Bohara, Yuki Kambe, Takashi Kurihara, Tetsuya Nagayama, Kazunori Arita, Atsuro Miyata
  • Organizer: 第86回 日本薬理学会年会
  • Place of Presentation: 福岡国際会議場,福岡
• [Presentation] C-TYPE NATRIURETIC PEPTIDE MODULATES THE PERMEABILITY OF BLOOD-BRAIN BARRIER (2013)
  • Author(s): Manoj Bohara
  • Organizer: 第86会 日本薬理学会年会
  • Place of Presentation: 福岡国際会議場
• [Presentation] PACAP による神経突起伸展促進作用におけるミトコンドリアの寄与 (2012)
  • Author(s): 神戸悠輝,宮田篤郎
  • Organizer: ニューロフォーラム
  • Place of Presentation: 鹿児島大学鶴陵会館,鹿児島
  • Year and Date: 2012-07-10
• [Presentation] PACAP による神経突起伸展促進作用におけるミトコンドリアの寄与 (2012)
  • Author(s): 神戸悠輝,宮田篤郎
  • Organizer: GPCR 研究会
  • Place of Presentation: 日本科学未来館 みらい CAN ホール,東京
  • Year and Date: 2012-05-11
• [Presentation] Involvement of mitochondria in PACAP dependent neurite outgrowth (2012)
  • Author(s): Yuki Kambe,Atsuro Miyata
  • Organizer: Society for Neuroscience
  • Place of Presentation: New Orleans at the Ernest N. Morial Convention Center,アメリカ
• [Presentation] Sp1 serves pivotal role in negative regulation of PAC1 gene expression by involvement of transglutaminase 2 (2012)
  • Author(s): Ayako Miura, Yuki Kambe, Kazuhiko Inoue, Takashi Kurihara, Atsuro Miyata
  • Organizer: Society for Neuroscience
  • Place of Presentation: New Orleans at the Ernest N. Morial Convention Center,アメリカ
• [Presentation] PACAPによる神経突起伸展促進作用におけるミトコンドリアの寄与 (2012)
  • Author(s): 神戸悠輝
  • Organizer: GPCR研究会
  • Place of Presentation: 日本科学未来館 みらいCANホール
• [Presentation] PACAPによる神経突起伸展促進作用におけるミトコンドリアの寄与 (2012)
  • Author(s): 神戸悠輝
  • Organizer: ニューロフォーラム
  • Place of Presentation: 鹿児島大学 鶴陵会館
• [Presentation] Involvement of mitochondria in PACAP dependent neurite outgrowth (2012)
  • Author(s): Yuki Kambe
  • Organizer: Society for Neuroscience, 2012
  • Place of Presentation: New Orleans at the Ernest N. Morial Convention Center.
• [Presentation] Sp1 serves pivotal role in negative regulation of PAC1 gene expression by involvement of transglutaminase 2 (2012)
  • Author(s): Ayako Miura
  • Organizer: Society for Neuroscience, 2012
  • Place of Presentation: New Orleans at the Ernest N. Morial Convention Center.
• [Presentation] PAC1 受容体を介した神経突起形成メカニズムにおけるミトコンドリア機能調節 (2011)
  • Author(s): 神戸悠輝,井上和彦,栗原崇,宮田篤郎
  • Organizer: 第64回日本薬理学会西南部会
  • Place of Presentation: KKR ホテル博多,福岡
  • Year and Date: 2011-11-20
• [Presentation] PAC1受容体を介した神経突起形成メカニズムにおけるミトコンドリア機能調節 (2011)
  • Author(s): 神戸悠輝, 井上和彦, 栗原崇, 宮田篤郎
  • Organizer: 第64回日本薬理学会西南部会
  • Place of Presentation: KKRホテル博多,福岡
  • Year and Date: 2011-11-20
• [Presentation] Neuro2a 細胞の神経突起形成過程における Pgc1 の細胞内輸送によるシグナル伝達機構の解明 (2011)
  • Author(s): 神戸悠輝,井上和彦,栗原崇,宮田篤郎
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: パシフィコ横浜,横浜
• [Presentation] PAC1 遺伝子の負の発現調節機序の解析 (2011)
  • Author(s): 三浦綾子,井上和彦,神戸悠輝,栗原崇,宮田篤郎
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: パシフィコ横浜,横浜
• [Presentation] Pgc1 α translocation may be one of the key factors for neurite outgrowth in neurons (2011)
  • Author(s): Yuki Kambe, Kazuhiko Inoue, Takashi Kurihara and Atsuro Miyata
  • Organizer: 8th Conference of Asian Society for Mitochondrial Research and Medicine
  • Place of Presentation: 市民文化ホール,鹿児島
• [Presentation] Intra-cellular translocation of Pgc1α is involved in PACAP induced neurite outgrowth in neuronal cells (2011)
  • Author(s): Yuki Kambe, Kazuhiko Inoue, Takashi Kurihara and Atsuro Miyata
  • Organizer: 第54回日本神経化学会大会
  • Place of Presentation: 瑠璃光,石川
• [Presentation] INVOLVEMENT OF MITOCHONDRIA IN PACAP-PAC1 DEPENDENT NEURITE OUTGROWTH (2011)
  • Author(s): Yuki Kambe, Kazuhiko Inoue, Takashi Kurihara, Atsuro Miyata
  • Organizer: 10th International Symposium on VIP, PACAP and Related Peptides
  • Place of Presentation: Hilton Eilat Queen of Sheba Hotel,イスラエル
• [Presentation] Pgc1α translocation may be one of the key factors for neurite outgrowth in neurons (2011)
  • Author(s): Yuki Kambe, Kazuhiko Inoue, Takashi Kurihara, Atsuro Miyata
  • Organizer: 8th Conference of Asian Society for Mitochondrial Research and Medicine
  • Place of Presentation: 市民文化ホール,鹿児島
• [Presentation] Intra-cellular translocation of Pgc1a is involved in PACAP in duced neurite outgrowth in neuronal cells (2011)
  • Author(s): Yuki Kambe, Kazuhiko Inoue, Takashi Kurihara, Atsuro Miyata
  • Organizer: 第54回日本神経化学会大会
  • Place of Presentation: 瑠璃光,石川
• [Presentation] INVOLVEMENT OF MITOCHONDEIA IN PACAP-PAC1 DEPENDENT NEURITE OUTGROWTH (2011)
  • Author(s): Yuki Kambe, Kazuhiko Inoue, Takashi Kurihara, Atsuro Miyata
  • Organizer: 10th International Symposium on VIP, PACAP and Related Peptides
  • Place of Presentation: Hilton Eilat Queen of Sheba Hotel,イスラエル
• [Presentation] Neuro2a細胞の神経突起形成過程におけるPgc1の細胞内輸送によるシグナル伝達機構の解明 (2011)
  • Author(s): 神戸悠輝, 井上和彦, 栗原崇, 宮田篤郎
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: パシフィコ横浜(横浜)(誌上開催)
• [Presentation] PAC1遺伝子の負の発現調節機序の解析 (2011)
  • Author(s): 三浦綾子, 井上和彦, 神戸悠輝, 栗原崇, 宮田篤郎
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: パシフィコ横浜(横浜)(誌上開催)
• [Presentation] From mitochondria to nucleus, Pgc-1 translocation may be one of the key factors for neurite extension in N2a cells (2010)
  • Author(s): Yuki Kambe, Atsuro Miyata
  • Organizer: 40^<th> annual meeting Neuroscience 2010
  • Place of Presentation: SanDiego, CA, USA
  • Year and Date: 2010-11-13
• [Presentation] From mitochondria to nucleus, Pgc-1 translocation may be one of the key factors for neurite extension in N2a cells (2010)
  • Author(s): Yuki Kambe and Atsuro Miyata
  • Organizer: 40th annual meeting Neuroscience
  • Place of Presentation: SanDiego, アメリカ
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