Establishment of isolation of amnion derived stem cells

• Project/Area Number: 22790277
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: University of Toyama
• Principal Investigator: KOIKE Chika 富山大学, 大学院・医学薬学研究部(医学), 助教 (10523889)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 再生医学 / 羊膜由来細胞 / 体性幹細胞 / 表面マーカー

Research Abstract

The stemness gene expression of human amnion epithelial cells （HAE) and mesenchymal cells （HAM) was compared with that of iPS cells and bone marrow derived mesenchymal stem cells （BMMSC), and was higher than BMMSC. It was suggested that HAE and HAM are closer to iPS cells than BMMSC. Drug treatment and Oct3/4 gene introduction into HAE cells made their stemness gene expression increased. Stem cells in HAE cells could be isolated according to TRA1-60 expression. HAM cells may have different differentiation potency according to their cell size.

Research Products

• [Journal Article] Establishment and Characterization of Immortalized Human Amniotic Epithelial Cells (2013)
  • Author(s): Kaixuan Zhou, Chika Koike, To shi koYoshida, Motonori Okabe,Moustafa Fathy, Satoshi Kyo,To ru Kiyono, Shigeru Saito, and Toshio Nikaido
  • Journal Title: Cellular Reprogramming Volume: 15 Pages: 55-67
  • Peer Reviewed
• [Journal Article] Selective isolation of Nanog positive human amniotic mesenchymal cells anddifferentiation into cardiomyocytes. (2013)
  • Author(s): Shingo Otaka., Saori Nagura, Chika Koike., Motonori Okabe, Toshiko Yoshida, Moustafa Fathy , Kentoku Yanagi, MisakiT.,and Toshio Nikaido
  • Journal Title: Cellular Reprogramming Volume: 15 Pages: 80-91
  • Peer Reviewed
• [Journal Article] Application of human amniotic mesenchymal cells as an allogenic transplantation cell source in bone regenerative therapy. (2012)
  • Author(s): Hiroaki Ttsuno, Toshiko Yoshida, Makiko Nogami, Chika Koike, Motonori Okabe, Zenkou Noto, Naoya Arai, Makoto Noguchi, Toshio Nikaido.
  • Journal Title: Material Science and Engineering C Volume: 32 Pages: 2452-2458
  • Peer Reviewed
• [Journal Article] Isolation and Characterization of Human Amniotic Mesenchymal Stem Cells and Their Chondrogenic Differentiation (2012)
  • Author(s): Makiko Nogami, Hiroaki Tsuno, Chika Koike, Motonori Okabe, Toshiko Yo shi d a,Shoji Seki, Yoshito Matsui, Tomoatsu Kimura, To shi o Nikaido
  • Journal Title: Transplantation. Volume: 93 (12) Pages: 1221-1228
  • Peer Reviewed
• [Presentation] 羊膜由来幹細胞単離マーカーの探索 (2013)
  • Author(s): 小池千加、吉田淑子、杉本潤、岡部素典、二階堂敏雄
  • Organizer: 第12回日本再生医療学会
  • Place of Presentation: 横浜
• [Presentation] ヒト羊膜間葉系幹細胞が肝臓線維化におよぼす効果 (2013)
  • Author(s): 吉田淑子、岡部素典、小池千加、王芳、斎藤滋、二階堂敏雄
  • Organizer: 第12回日本再生医療学会
  • Place of Presentation: 横浜
• [Presentation] REPROGRAMING OF HUMAN AMNIOTIC EPITHELIAL CELLS BY OCT4 OVEREXPRESSION (2012)
  • Author(s): Chika Koike, Toshiko Yoshida, Motonori Okabe, Toshio Nikaido
  • Organizer: The 10th meeting of International Society for Stem Cell Research
  • Place of Presentation: Yokohama
• [Presentation] REPROGRAMING OF HUMAN AMNIOTIC EPITHELIAL CELLS BY OCT4 OVEREXPRESSION (2012)
  • Author(s): Chika Koike
  • Organizer: The 10th meeting of International Society for Stem Cell Research
  • Place of Presentation: 横浜
• [Presentation] Reprograming of human amniotic epithelial cells by Oct4 overexpression (2011)
  • Author(s): Yuji Takeda, Chika Koike, 他
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜横浜
  • Year and Date: 2011-12-14
• [Presentation] 羊膜由来細胞のStemnessの度合いの解析 (2011)
  • Author(s): 小池千加, 他
  • Organizer: 第29回日本ヒト細胞学会
  • Place of Presentation: 高志会館富山
  • Year and Date: 2011-08-20
• [Presentation] Oct-4過剰発現による不死化ヒト羊膜上皮細胞のりプログラミング (2011)
  • Author(s): 竹田裕司, 小池千加, 他
  • Organizer: 再生医療学会
  • Place of Presentation: 京王プラザホテル 東京
  • Year and Date: 2011-03-01
• [Presentation] Establishment of insulin expressing amniotic cells and cell therapy for diabetes (2011)
  • Author(s): Chika Koike, Jing Fang, Toshiko Yoshida, Kaizuan Zho, Motonori Okabe, and Toshio Nikaido
  • Organizer: 第10回日本再生医療学会
  • Place of Presentation: 東京
• [Presentation] Reprogramming of human amniotic epithelial cells by Oct4 overexpression. (2011)
  • Author(s): Toshio Nikaido, Yuji Takeda, Chika Koike,Toshiko Yoshida, Motonori Okabe
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] Oct-4過剰発現による不死化ヒト羊膜上皮細胞のりプログラミング (2011)
  • Author(s): 竹田裕司、小池千加、吉田淑子、岡部素典、杉本潤、二階堂敏雄
  • Organizer: 第10回日本再生医療学会
  • Place of Presentation: 東京
• [Presentation] 不死化羊膜上皮細胞の樹立と解析 (2011)
  • Author(s): 周凱旋、小池千加、吉田淑子、岡部素典、京哲、清野透、二階堂敏雄
  • Organizer: 第10回日本再生医療学会
  • Place of Presentation: 東京
• [Presentation] 羊膜間葉系幹細胞の単離・同定と軟骨分化に有効な成長因子の検討 (2011)
  • Author(s): 野上真紀子、津野宏彰、Moustafa Fathy Omar、小池千加、岡部素典、吉田淑子、関庄二、松井好人、木村友厚、二階堂敏雄
  • Organizer: 第29回日本ヒト細胞学会
  • Place of Presentation: 富山
• [Presentation] 二階堂敏雄 羊膜由来細胞のStemnessの度合いの解析 (2011)
  • Author(s): 小池千加、周凱旋、岡部素典、吉田淑子
  • Organizer: 第29回日本ヒト細胞学会
  • Place of Presentation: 富山
• [Presentation] Characterization of Amniotic Stem Cells (2010)
  • Author(s): Chika Koike, Kaixuan Zho, Moustafa Fathy, Motonori Okabe, Toshiko Yoshida, and Toshio NIkaido.他
  • Organizer: The 8thInternational Society for Stem Cell Research
  • Place of Presentation: San Francisco, CA, U.S.A
  • Year and Date: 2010-06-18
• [Presentation] Characterization of Amniotic Stem Cells (2010)
  • Author(s): Chika Koike, 他
  • Organizer: International Society for Stem Cell Research
  • Place of Presentation: Moscone West, San Francisco.CA, U.S.A
  • Year and Date: 2010-06-18
• [Book] 無敵のバイオテクニカルシリーズ「改訂第3版 遺伝子工学実験ノート」(田村隆明 編) (2010)
  • Author(s): 小池千加
  • Publisher: 羊土社
• [Patent(Industrial Property Rights)] 羊膜間葉系幹細胞の調製方法および単離された羊膜間葉系幹細胞集団 (2011)
  • Inventor(s): 二階堂敏雄、吉田淑子、岡部素典、小池千加、野上真紀子、木村友厚、野口誠、津野宏彰、竹田裕治
  • Industrial Property Rights Holder: 富山大学
  • Industrial Property Number: 2011-257419
  • Filing Date: 2011-11-25