Analysis of Myc function during reprogramming of somatic cell nuclear

• Project/Area Number: 22790282
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Kyoto University
• Principal Investigator: NAKAGAWA Masato 京都大学, iPS細胞研究所, 講師 (10379539)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 再生医学 / リプログラミング / iPS細胞 / ES細胞 / 癌

Research Abstract

Previously c-Myc is thought to be one of the risks for tumorigenicity of iPS-derived chimera mice. To overcome this problem, we found that c-Myc can be replaced with L-Myc. From detailed study, a strong transforming activity of c-Myc negatively acted in the early establishment of iPS cells. On the other hand, little transforming activity of L-Myc efficiently inhibited the expression of a gene highly expressed in fibroblasts resulted in promotion of the establishment of iPS cells.

Research Products

• [Journal Article] Specific lectin biomarkers for isolation of human pluripotent stem cells identified through array-based glycomic analysis (2011)
  • Author(s): Wang YC, Nakagawa M, Garitaonandia I, Slavin I, Altun G, Lacharite RM, Nazor KL, Tran HT, Lynch CL, Leonardo TR, Liu Y, Peterson SE, Laurent LC, Yamanaka S, & Loring JF
  • Journal Title: Cell Res
  • Peer Reviewed
• [Journal Article] A more efficient method to generate integration-free human iPS cells (2011)
  • Author(s): Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong H, Nakagawa M, Tanabe K, Tezuka K, Shibata T, Kunisada T, Takahashi M, Takahashi J, Saji H, & Yamanaka S.
  • Journal Title: Nat Methods Volume: 8(5) Pages: 409-412
  • NAID: 120002949763
  • Peer Reviewed
• [Journal Article] Essential roles of ECAT15-2/Dppa2 in functional lung development (2011)
  • Author(s): Nakamura T, Nakagawa M, Ichisaka T, Shiota A, & Yamanaka S.
  • Journal Title: Mol Cell Biol
  • NAID: 120003674151
  • Peer Reviewed
• [Journal Article] Human and Mouse Induced Pluripotent Stem Cells Are Differentially Reprogrammed in response to Kinase Inhibitors (2011)
  • Author(s): Hirano K, Nagata S, Yamaguchi S, Nakagawa M, Okita K, Kotera H, Ainscough J, & Tada T
  • Journal Title: Stem Cells Dev
  • NAID: 120004247259
  • Peer Reviewed
• [Journal Article] Induction and enhancement of cardiac cell differentiation from mouse and human induced pluripotent stem cells with cyclosporin-A (2011)
  • Author(s): Fujiwara M, Yan P, Otsuji TG, Narazaki G, Uosaki H, Fukushima H, Kuwahara K, Harada M, Matsuda H, Matsuoka S, Okita K, Takahashi K, Nakagawa M, Ikeda T, Sakata R, Mummery CL, Nakatsuji N, Yamanaka S, Nakao K, & Yamashita JK.
  • Journal Title: PLoS One Volume: 6(2)
  • Peer Reviewed
• [Journal Article] Promotion of direct reprogramming by transformation-deficient Myc (2010)
  • Author(s): Nakagawa M, Takizawa N, Narita M, Ichisaka T & Yamanaka S.
  • Journal Title: Proc Natl Acad Sci USA Volume: 107 Pages: 14152-14157
  • Peer Reviewed
• [Journal Article] miRNAs regulate SIRT1 expression during mouse embryonic stem cell differentiation and in adult mouse tissues (2010)
  • Author(s): Saunders LR, Sharma AD, Tawney J, Nakagawa M, Okita K, Yamanaka S, Willenbring H, & Verdin E.
  • Journal Title: Aging(Albany NY) Pages: 415-431
  • Peer Reviewed
• [Journal Article] Complete genetic correction of ips cells from Duchenne muscular dystrophy (2010)
  • Author(s): Kazuki Y, Hiratsuka M, Takiguchi M, Osaki M, Kajitani N, Hoshiya H, Hiramatsu K, Yoshino T, Kazuki K, Ishihara C, Takehara S, Higaki K, Nakagawa M, Takahashi K, Yamanaka S, & Oshimura M.
  • Journal Title: Mol Ther Volume: 18 Pages: 386-393
  • Peer Reviewed
• [Journal Article] Promotion of direct reprogramming by transformation-deficient Myc. (2010)
  • Author(s): Nakagawa, M., Takizawa, N., Narita, M., Ichisaka, T., Yamanaka, S.
  • Journal Title: Proc Natl Acad Sci U S A Volume: 107 Pages: 14152-14157
  • Peer Reviewed
• [Journal Article] miRNAs regulate SIRT1 expression during mouse embryonic stem cell differentiation and in adult mouse tissues. (2010)
  • Author(s): Saunders, L.R., Sharma, A.D., Tawney, J., Nakagawa, M., Okita, K, Yamanaka., S, Willenbring., H, Verdin, E.
  • Journal Title: Aging (Albany NY) Volume: 2 Pages: 415-431
  • Peer Reviewed
• [Journal Article] Complete genetic correction of ips cells from Duchennemuscular dystrophy (2010)
  • Author(s): Kazuki, Y., Hiratsuka, M., Takiguchi, M., Osaki, M., Kajitani, N., Hoshiya, H., Hiramatsu, K., Yoshino, T., Kazuki, K., Ishihara, C., Takehara, S., Higaki, K., Nakagawa, M., Takahashi, K., Yamanaka, S., Oshimura, M.
  • Journal Title: Mol Ther Volume: 18 Pages: 386-393
  • Peer Reviewed
• [Presentation] Function of Myc for Reprogramming. (2010)
  • Author(s): Nakagawa M, et al.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2010-12-13
• [Presentation] Promotion of Direct Reprogramming by Transformation-deficient Myc (2010)
  • Author(s): Nakagawa M, et al.
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] Promotion of Direct Reprogramming by Transformation-deficient Myc. (2010)
  • Author(s): Nakagawa M, et al.
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] Generation of safer iPS cells by factor X instead of c-Myc (2010)
  • Author(s): Nakagawa M, et al.
  • Organizer: ISSCR 8th Annual Meeting
  • Place of Presentation: San Francisco
  • Year and Date: 2010-06-17
• [Presentation] Generation of safer iPS cells by factor X instead of c-Myc. (2010)
  • Author(s): Nakagawa M, et al.
  • Organizer: ISSCR 8th Annual Meeting
  • Place of Presentation: San Francisco
  • Year and Date: 2010-06-17
• [Book] Reprogramming of somatic cells to pluripotency (2010)
  • Author(s): Nakagawa M, Yamanaka S.
  • Publisher: Adv Exp Med Biol
• [Book] Reprogramming of somatic cells to pluripotency (2010)
  • Author(s): Nakagawa M, Yamanaka S
  • Publisher: Adv Exp Med Biol
• [Remarks]
  • URL: http://www.cira.kyoto-u.ac.jp/j/index.html
• [Remarks]
  • URL: http://www.cira.kyoto-u.ac.jp/j/index.html