Cell therapy for Muscular Dystrophy using myogenic progenitor cells derived from human iPS cells

• Project/Area Number: 22790284
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Kyoto University
• Principal Investigator: SAKURAI Hidetoshi 京都大学, iPS細胞研究所, 講師 (80528745)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: iPS細胞 / 筋ジストロフィー / 再生医療 / 骨格筋前駆細胞 / 分化誘導 / 筋ジストロフィーモデル / 中胚葉誘導

Research Abstract

We developed an efficient and simple method to generate integration-free human iPSCs with episomal plasmid vectors from multiple donors, including two in which only one HLA haplotype was detected. These iPSCs were effectively differentiated into various cell types in vivo and in vitro. The two putatively HLA-homozygous iPSC lines match~ 20% of the Japanese population at major HLA loci.

Research Products

• [Presentation] 転写因子を用いた効率的なヒトiPS細胞からの骨格筋細胞誘導法の確立 (2011)
  • Author(s): 田中章仁、櫻井英俊
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-13
• [Presentation] iPS細胞からの中胚葉前駆細胞誘導と、細胞移植治療に向けた研究開発 (2010)
  • Author(s): 櫻井英俊
  • Organizer: 筋ジストロフィーに対するトランスレーショナル・リサーチ
  • Place of Presentation: 東京
  • Year and Date: 2010-12-13
• [Presentation] Transplantation of mouse iPS cell-derived mesodermal progenitors improves structure and function of skeletal muscle in Duchenne Muscular Dystrophy model mice (2010)
  • Author(s): 櫻井英俊
  • Organizer: 8th International Society for Stem Cell Research Annual Meeting
  • Place of Presentation: トロント(カナダ)
  • Year and Date: 2010-06-18
• [Presentation] Transplantation of mouse iPS cell-derived mesodermal progenitors improves structure and function of skeletal muscle in Duchenne Muscular Dystrophy model mice (2010)
  • Author(s): 櫻井英俊
  • Organizer: The 8^<th> Annual Meeting of International Society for Stem Cell Research (ISSCR)
  • Place of Presentation: Moscone West(サンフランシスコ)
  • Year and Date: 2010-06-18
• [Remarks]
  • URL: http://www.cira.kyoto-u.ac.jp/sakurai/
• [Remarks]
  • URL: http://www.cira.kyoto-u.ac.jp/j/research/sakurai_summary.html
• [Patent(Industrial Property Rights)] METHOD OF INDUCING DIF FERENTIATION FROM PLUR IPOTENT STEM CELLS TO SKELETAL MUSCLE CELLS (2011)
  • Inventor(s): 横井英俊、田中章仁、Knut Woltjen, 池谷真
  • Industrial Property Rights Holder: 京都大学
  • Filing Date: 2011-11-18
  • Overseas
• [Patent(Industrial Property Rights)] METHOD OF INDUCING DIFFERENTIATION FROM PLURIPOTENT STEM CELL TO SKELETAL MUSCLE PROGENITOR CELLS (2010)
  • Inventor(s): 櫻井英俊, 坂口泰子, 瀬原淳子
  • Industrial Property Rights Holder: 京都大学
  • Filing Date: 2010-07-09
  • Overseas