Identification of reprogramming inhibitory factors by ES cell differentiation

• Project/Area Number: 22790285
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Kyoto University
• Principal Investigator: OKITA Keisuke 京都大学, iPS細胞研究所, 講師 (90512434)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 人工多能性幹細胞 / iPS細胞 / 初期化 / ベクター / 再生医療

Research Abstract

We developed an efficient and simple method to generate integration-free human iPSCs with episomal plasmid vectors from multiple donors, including two in which only one HLA haplotype was detected. These iPSCs were effectively differentiated into various cell types in vivo and in vitro. The two putatively HLA-homozygous iPSC lines match~ 20% of the Japanese population at major HLA loci.

Research Products

• [Journal Article] A more efficient method to generate integration-free human iPS cells (2011)
  • Author(s): Okita K, et al.
  • Journal Title: Nat. Methods Volume: 8 Pages: 409-412
  • NAID: 120002949763
  • URL: http://repository.kulib.kyoto-u.ac.jp/dspace/handle/2433/139494
  • Peer Reviewed
• [Journal Article] A more efficient method to generate integration-free human iPS cells (2011)
  • Author(s): Keisuke Okita, et al
  • Journal Title: Nature Methods Volume: 8 Pages: 409-412
  • NAID: 120002949763
  • Peer Reviewed
• [Journal Article] A more efficient method to generate integration-free human iPS cells (2011)
  • Author(s): Okita K, et al.
  • Journal Title: Nature Methods
  • NAID: 120002949763
  • Peer Reviewed
• [Journal Article] Gingival fibroblasts as a promising source of induced pluripotent stem cells (2010)
  • Author(s): Egusa H, Okita K, et al.
  • Journal Title: PLoS One Volume: 5
  • Peer Reviewed
• [Presentation] iPS細胞とは (2012)
  • Author(s): 沖田圭介
  • Organizer: 福岡大学iPS細胞研究ワークショップ-iPS細胞実用化の最先端を語る-
  • Place of Presentation: 福岡(招待講演)
  • Year and Date: 2012-03-16
• [Presentation] Generation of iPS cells for therapeutic treatment (2012)
  • Author(s): Okita K
  • Organizer: Interdisciplinary approaches for the study of senescence
  • Place of Presentation: University of Cambridge, UK
  • Year and Date: 2012-02-09
• [Presentation] Generation of iPS cells for therapeutic treatment (2012)
  • Author(s): Keisuke Okita
  • Organizer: Interdisciplinary approaches for the study of senescence
  • Place of Presentation: University of Cambridge, UK(招待講演)
  • Year and Date: 2012-02-09
• [Presentation] iPS細胞の臨床応用を目指して (2011)
  • Author(s): 沖田圭介
  • Organizer: 再生医療の実現化プロジェクトiPS細胞等研究拠点事業平成23年度東京大学全体会議
  • Place of Presentation: 千葉(招待講演)
  • Year and Date: 2011-09-13
• [Presentation] iPS細胞の臨床応用を目指して (2011)
  • Author(s): 沖田圭介
  • Organizer: 第21回日本サイトメトリー学会学術集会(招待講演)
  • Place of Presentation: 京都
  • Year and Date: 2011-06-25
• [Presentation] iPS細胞の臨床応用を目指して (2011)
  • Author(s): 沖田圭介
  • Organizer: 第21回日本サイトメトリー学会学術集会
  • Place of Presentation: 京都(招待講演)
  • Year and Date: 2011-06-25
• [Presentation] 非ウイルスベクターによる血液細胞からのヒトiPS細胞の樹立 (2011)
  • Author(s): 沖田圭介他
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-03-02
• [Presentation] Generation of iPS cells for therapeutic treatment (2011)
  • Author(s): Keisuke Okita
  • Organizer: Heidelberg-Kyoto Joint Symposium "Crossing Boundaries : stem Cells, Materials, and Hesoscopic Sciences"
  • Place of Presentation: Heidelberg, Germany(招待講演)
• [Presentation] Human iPS induction from adult fibroblasts in virus free condition (2010)
  • Author(s): Okita K, et al.
  • Organizer: Biochemistry and Molecular biology 2010
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] 人工多能性幹(iPS)細胞の課題と展望 (2010)
  • Author(s): 沖田圭介
  • Organizer: 日本放射線影響学会第53回大会(招待講演)
  • Place of Presentation: 京都
  • Year and Date: 2010-10-21
• [Presentation] 人工多能性幹(iPS)細胞の課題と展望 (2010)
  • Author(s): 沖田圭介
  • Organizer: 日本放射線影響学会第53回大会
  • Place of Presentation: 京都(招待講演)
  • Year and Date: 2010-10-21
• [Book] 「分子から個体へと進化する脂質生物学」の「プロスタノイドによる免疫系・神経系制御」の章 (2012)
  • Author(s): 青井貴之、大貫茉里、沖田圭介
  • Publisher: 羊土社
• [Book] Principles of Regenerative Medicine. Second edition, Chapter13 Induced pluripotent stem cells (2010)
  • Author(s): Okita K, Yamanaka S.
  • Publisher: Academic press
• [Book] Principles of Regenerative Medicine, Second edition (2010)
  • Author(s): Keisuke Okita, Shinya Yamanaka
  • Total Pages: 1182
  • Publisher: Academic press
• [Remarks]
  • URL: http://www.cira.kyoto-u.ac.jp/j/index.html
• [Remarks]
  • URL: http://www.cira.kyoto-u.ac.jp/j/index.html
• [Patent(Industrial Property Rights)] 効率的な人工多能性幹細胞の樹立方法 (2010)
  • Inventor(s): 沖田圭介/中川誠人/山中伸弥
  • Industrial Property Rights Holder: 国立大学法人京都大学
  • Filing Date: 2010-08-06