| Project/Area Number |
22790287
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
|
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 再生医学 / 再生医療 / 遺伝子発現 / 多能性幹細胞 |
|---|
| Research Abstract |
Forced expression of a set of transcription factors induces somatic cells into pluripotent stem cells(iPS cells). Their morphology, differentiation capacity and gene expression profile are almost exactly equivalent to those in embryonic stem(ES) cells. In order to apply iPS cell technology in regenerative medicine, high-quality iPS-cells should be stably generated. To achieve this, high accuracy evaluation methods are needed to assess the differentiation capacity and tumorigenesis risk in generated iPS cells. In this study, we performed gene expression analysis at the single cell level and developed the novel evaluation method for iPS cells quality.
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