Study of the mechanism by which phospholipase C・regulates T-cell-mediated inflammation

• Project/Area Number: 22790290
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Kobe University
• Principal Investigator: EDAMATSU Hironori 神戸大学, 大学院・医学研究科, 助教 (70335438)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: シグナル伝達 / 免疫 / アレルギー / 炎症 / モデル動物 / 癌 / サイトカイン / 細胞内シグナル伝達 / Ras / T細胞 / 喘息 / 乾癬 / CD4陽性細胞 / 疾患モデル動物 / 非免疫細胞 / ホスホリパーゼ

Research Abstract

Phospholipase C.(PLC.) is an intracellular signal transmitter involved in regulation of inflammatory cytokine production. In this study, it was shown that PLC. cooperates with the NF-κB pathway to upregulate inflammatory cytokine production in epidermal keratinocytes. In the transgenic mice overexpressing PLC. in the epidermal keratinocytes, the development of dermatitis requires the increased production of interleukin 23 by the keratinocytes.

Research Products

• [Journal Article] Phospholipase C・as a potential molecular target for anti-inflammatory therapy and cancer prevention (2011)
  • Author(s): Edamatsu, H., Takenaka, N., Hu, L., and Kataoka, T.
  • Journal Title: Inflamm. Regen Volume: 31 Pages: 370-374
  • Peer Reviewed
• [Journal Article] PLC・cooperates with the NF-・B pathway to augment TNF・-stimulated CCL2/MCP1 expression in human keratinocyte (2011)
  • Author(s): Harada, Y., Edamatsu, H., and Kataoka, T.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 414 Pages: 106-111
  • Peer Reviewed
• [Journal Article] Phospholipase C・has a crucial role in ultraviolet B-induced neutrophil-associated skin inflammation by regulating the expression of CXCL1/KC (2011)
  • Author(s): Oka, M., Edamatsu, H., Kunisada, M., Hu, L., Takenaka, N., Sakaguchi, M., Kataoka, T., and Nishigori, C.
  • Journal Title: Lab. Invest Volume: 91 Pages: 711-718
  • Peer Reviewed
• [Journal Article] Overexpression of phospholipase C・in keratinocytes upregulates cytokine expression and causes dermatitis with acanthosis and T-cell infiltration (2011)
  • Author(s): Takenaka, N.*, Edamatsu, H.*, Suzuki, N., Saito, H., Inoue, Y., Oka, M., Hu, L., and Kataoka T.
  • Journal Title: Eur. J. Immunol Volume: 41 Pages: 202-213
  • Peer Reviewed
• [Journal Article] Enhancement of ultraviolet B-induced skin tumor development in phospholipase C・knockout mice is associated with decreased cell death (2011)
  • Author(s): Oka, M., Edamatsu, H., Kunisada, M., Hu, L., Takenaka, N., Dien, S., Sakaguchi, M., Kitazawa, R., Norose, K., Kataoka, T., and Nishigori, C.
  • Journal Title: Carcinogenesis Volume: 31 Pages: 1897-1902
  • Peer Reviewed
• [Journal Article] PLCε cooperates with the NF-κB pathway to augment TNFα-stimulated CCL2/MCP1 expression in human keratinocyte (2011)
  • Author(s): Harada, Y., Edamatsu, H., Kataoka, T.
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 414 Issue: 1 Pages: 106-111
  • DOI: 10.1016/j.bbrc.2011.09.032
  • Peer Reviewed
• [Journal Article] Phospholipase Cε has a crucial role in ultraviolet B-induced neutrophil-associated skin inflammation by regulating the expression of CXCL1/KC (2011)
  • Author(s): Oka, M., Edamatsu, H., et al
  • Journal Title: Lab.Invest. Volume: 91 Issue: 5 Pages: 711-718
  • DOI: 10.1038/labinvest.2011.10
  • Peer Reviewed
• [Journal Article] Phospholipase Cε as a potential molecular target for anti-inflammatory therapy and cancer prevention (2011)
  • Author(s): Edamatsu, H., Takenaka, N., Hu, L., Kataoka, T.
  • Journal Title: Inflamm.Regen. Volume: 31 Pages: 370-374
  • NAID: 130004482284
  • Peer Reviewed
• [Journal Article] Overexpression of phospholipase Cε in keratinocytes upregulates cytokine expression and causes dermatitis with acanthosis and T-cell infiltration. (2011)
  • Author(s): Takenaka, N., Edamatsu, H., et al.
  • Journal Title: Eur.J.Immunol. Volume: 41 Pages: 203-213
  • Peer Reviewed
• [Journal Article] Enhancement of ultraviolet B-induced skin tumor development in phospholipase Cε-knockout mice is associated with decreased cell death. (2010)
  • Author(s): Oka, M., Edamatsu, H., et al.
  • Journal Title: Carcinogenesis Volume: 31 Pages: 1897-1902
  • Peer Reviewed
• [Journal Article] Phospholipase Cε has a crucial role in ultraviolet B-induced neutrophil-associated skin inflammation by regulating the expression of CXCL1/KC.
  • Author(s): Oka, M., Edamatsu, H., et al.
  • Journal Title: Lab.Invest. Volume: (印刷中)
  • Peer Reviewed
• [Presentation] Crucial role of phospholipase C・in the development of asthma in mice (2011)
  • Author(s): Nagano, T., Edamatsu, H., Kobayashi, K., Nishimura, Y., and Kataoka, T
  • Organizer: European Respiratory Society Annual Congress(欧州呼吸器学会年会)
  • Place of Presentation: オランダアムステルダム
  • Year and Date: 2011-09-25
• [Presentation] Crucial role of phospholipase Cε in the development of asthma in mice (2011)
  • Author(s): Nagano, T., Edamatsu, H., Kobayashi, K., Nishimura, Y, Kataoka, T.
  • Organizer: European Respiratory Society Annual Congress(欧州呼吸器学会年会)
  • Place of Presentation: Amsterdam RAI(オランダ、アムステルダム市)
  • Year and Date: 2011-09-25
• [Presentation] Overexpression of phospholipase C・in keratinocytes results in IL-23 production leading to skin inflammation accompanied by IL-22-producing T cell infiltration (2011)
  • Author(s): Edamatsu, H., Takenaka, N., and Kataoka, T
  • Organizer: 10th World Congress on Inflammation(第10回国際炎症学会)
  • Place of Presentation: フランスパリ
  • Year and Date: 2011-06-27
• [Presentation] Overexpression of phospholipase Cε in keratinocytes results in IL-23 production leading to skin inflammation accompanied by IL-22-producing T cell infiltration (2011)
  • Author(s): Edamatsu, H., Takenaka, N., Kataoka, T.
  • Organizer: 10th World Congress on Inflammation(第10回国際炎症学会)
  • Place of Presentation: Paris Convention Centre(フランス、パリ市)
  • Year and Date: 2011-06-27
• [Presentation] Acanthosis accompanied by aberrant infiltration of IL-22-producing T cells in the transgenic mice overexpressing phospholipase C・in the epidermis (2010)
  • Author(s): Edamatsu, H., Takenaka, N., Suzuki, N, . Saito, H., Inoue, Y., Oka, M., Hu, L., and Kataoka, T
  • Organizer: 14th International Congress of Immunology(第14回国際免疫学会議)
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-25
• [Presentation] Acanthosis accompanied by aberrant infiltration of IL-22-producing T cells in the transgenic mice overexpressing phospholipase Cε in the epidermis (2010)
  • Author(s): Edamatsu, H., et al.
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: 神戸ポートピアホテル、神戸国際展示場(兵庫県)
  • Year and Date: 2010-08-25
• [Presentation] ホスホリパーゼC・の炎症応答・発癌における重要な役割と創薬標的としての可能性 (2010)
  • Author(s): 枝松裕紀、竹中延之、胡立志、片岡徹
  • Organizer: 第31回日本炎症・再生医学会
  • Place of Presentation: 東京
  • Year and Date: 2010-08-05
• [Presentation] ホスホリパーゼCεの炎症応答・発癌における重要な役割と創薬標的としての可能性 (2010)
  • Author(s): 枝松裕紀, 他
  • Organizer: 第31回日本炎症・再生医学会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2010-08-05
• [Remarks]
  • URL: http://www.med.kobe-u.ac.jp/molbiol/index.html
• [Remarks]
  • URL: http://www.med.kobe-u.ac.jp/molbiol/index.html
• [Remarks]
  • URL: http://www.med.kobe-u.ac.jp/molbiol/index.html