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Analysis of the lysosomal enzyme metabolizing the anti-inflammatory lipid, N-acylethanolamine

Research Project

Project/Area Number 22790295
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionKagawa University

Principal Investigator

TSUBOI Kazuhito  香川大学, 医学部, 助教 (80346642)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords脂質 / 酵素 / 生理活性 / 生体分子
Research Abstract

In the present study, we analyzed enzymes metabolizing the anti-inflammatory lipid, N-acylethanolamine(NAE). We found that NAE-hydrolyzing acid amidase(NAAA), a lysosomal enzyme degrading NAEs, is activated by endogenous substances such as some phospholipids and dihydrolipoic acid and that several lipophilic amines are new inhibitors of NAAA. Furthermore, we suggested that HRASLS1, a member of the tumor suppressor HRASLS family, is involved in NAE biosynthesis by its N-acyltransferase activity.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (52 results)

All 2012 2011 2010 Other

All Journal Article (19 results) (of which Peer Reviewed: 16 results) Presentation (30 results) Remarks (3 results)

  • [Journal Article] Regulation of peroxisomal lipid metabolism by catalytic activity of tumor suppressor H-rev1072012

    • Author(s)
      Uyama, T., Ichi, I., Kono, N. Inoue, A., Tsuboi, K., Jin, X.-H., Araki, N., Aoki, J., Arai, H., and Ueda, N
    • Journal Title

      Journal of Biological Chemistry

      Volume: 287 Pages: 2706-2718

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Endogenous molecules stimulating N-acylethanolamine-hydrolyzing acid amidase(NAAA)2012

    • Author(s)
      Tai T, Tsuboi K, Uyama T, Ma Cravatt BF, Houchi H, Ueda N
    • Journal Title

      ACS Chem Neurosci

      Volume: (印刷中) Issue: 5 Pages: 379-385

    • DOI

      10.1021/cn300007s

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Lipophilic amines as potent inhibitors of N-acylethanolamine-hydrolyzing acid amidase2012

    • Author(s)
      Yamano, Y., Tsuboi, K., Hozaki, Y., Takahashi, K., Jin, X.-H., Ueda, N., and Wada, A
    • Journal Title

      Bioorganic & Medicinal Chemistry

      Volume: 20 Pages: 3658-3665

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Lipophilic amines as potent inhibitors of N-acylethanolamine-hydrolyzing acid amidase2012

    • Author(s)
      Yamano Y, Tsuboi K, Hozaki Y, Takahashi K, Jin X-H, Ueda N, Wada A
    • Journal Title

      Bioorg Med Chem

      Volume: 20(11) Issue: 11 Pages: 3658-3665

    • DOI

      10.1016/j.bmc.2012.03.065

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biosynthesis and degradation of the endocannabinoid 2-arachidonoylglycerol2011

    • Author(s)
      Ueda, N., Tsuboi, K., Uyama, T., and Ohnishi, T
    • Journal Title

      BioFactors

      Volume: 37 Pages: 1-7

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Studies on the anorectic effect of N-acylphosphatidylethanolamine and phosphatidylethanolamine in mice2011

    • Author(s)
      Wellner, N., Tsuboi, K., Madsen, A. N., Holst, B., Diep, T. A., Nakao, M., Tokumura, A., Burns, M. P., Deutsch, D. G., Ueda, N., and Hansen, H. S
    • Journal Title

      Biochimica et Biophysica Acta-Molecular and Cell Biology of Lipids

      Volume: 1811 Pages: 508-512

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enzymatic formation of N-acylethanol-amines from N-acylethanolamine plasmalogen through N-acylphosphati-dylethanol-amine-hydrolyzing phos-pholipase D-dependent and-independent pathways2011

    • Author(s)
      Tsuboi, K., Okamoto, Y., Ikematsu, N., Inoue, M., Shimizu, Y., Uyama, T., Wang, J., Deutsch, D. G., Burns, M. P., Ulloa, N. M., Tokumura, A., and Ueda, N
    • Journal Title

      Biochimica et Biophysica Acta-Molecular and Cell Biology of Lipids

      Volume: 1811 Pages: 565-577

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enzymological analysis of the tumor suppressor A-C1 reveals a novel group of phospholipid-metabolizing enzymes2011

    • Author(s)
      Shinohara, N., Uyama, T., Jin, X.-H., Tsuboi, K., Tonai, T., Houchi, H., and Ueda, N
    • Journal Title

      Journal of Lipid Research

      Volume: 52 Pages: 1927-1935

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 三本鎖のリン脂質N-アシルホスファチジルエタノールアミンの動物組織における代謝2011

    • Author(s)
      坪井一人, 宇山徹, 上田夏生
    • Journal Title

      生化学

      Volume: 83 Pages: 485-494

    • NAID

      10029068825

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 脂質メテ〓メィエーターとして機能するN-アシルエタノールアミンの分解酵素とその阻害薬2011

    • Author(s)
      坪井一人, 上田夏生
    • Journal Title

      日本薬理学雑誌

      Volume: 138 Pages: 8-12

    • Related Report
      2011 Final Research Report
  • [Journal Article] 食欲抑制メディエーターとしてのオレオイルエタノールアミド2011

    • Author(s)
      上田夏生, 坪井一人, 宇山徹
    • Journal Title

      ビタミン

      Volume: 85 Pages: 604-607

    • NAID

      110008762054

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Journal Article] Studies on the anorectic effect of N-acylphosphatidylethanolamine and phosphatidylethanolamine in mice2011

    • Author(s)
      Wellner N., et al
    • Journal Title

      Biochim Biophys Acta-Mol Cell Biol Lipids

      Volume: 1811 Issue: 9 Pages: 508-512

    • DOI

      10.1016/j.bbalip.2011.06.020

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enzymatic formation of N-acylethanolamines from N-acylethanolamine plasmalogen through N-acylphosphatidylethanolamine-hydrolyzing phospholipase D-dependent and -independent pathways2011

    • Author(s)
      Tsuboi, K., et al
    • Journal Title

      Biochim Biophys Acta-Mol Cell Biol Lipids

      Volume: 1811 Issue: 10 Pages: 565-577

    • DOI

      10.1016/j.bbalip.2011.07.009

    • NAID

      110009612886

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enzymological analysis of the tumor suppressor A-Cl reveals a novel group of phospholipid-metabolizing enzymes2011

    • Author(s)
      Naoki Shinohara, et al
    • Journal Title

      Journal of Lipid Research

      Volume: 52 Issue: 11 Pages: 1927-1935

    • DOI

      10.1194/jlr.m015081

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 脂質メティエーターとして機能するN-アシルエタノールアミンの分解酵素とその阻害薬2011

    • Author(s)
      坪井一人, ら
    • Journal Title

      日本薬理学雑誌

      Volume: 138 Pages: 8-12

    • Related Report
      2011 Annual Research Report
  • [Journal Article] N-acylethanolamine metabolism with special reference to N-acylethanol-amine-hydrolyzing acid amidase(NAAA)2010

    • Author(s)
      Ueda, N., Tsuboi, K., and Uyama, T
    • Journal Title

      Progress in Lipid Research

      Volume: 49 Pages: 299-315

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enzymological studies on the biosynthesis of N-acylethanolamines2010

    • Author(s)
      Ueda, N., Tsuboi, K., and Uyama, T
    • Journal Title

      Biochimica et Biophysica Acta-Molecular and Cell Biology of Lipids

      Volume: 1801 Pages: 1274-1285

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] N-acylethanolamine metabolism with special reference to N-acylethanolamine-hydrolyzing acid amidase (NAAA)2010

    • Author(s)
      Ueda, N., et al.
    • Journal Title

      Progress in Lipid Research

      Volume: 49 Pages: 299-315

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enzymological studies on the biosynthesis of N-acylethanolamines2010

    • Author(s)
      Ueda, N., et al.
    • Journal Title

      Biochimica et Biophysica Acta

      Volume: 1801 Pages: 1274-1285

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] マウス全脳におけるエタノールアミン含有脂質の分子種分析と関連酵素の基質特異性解析2011

    • Author(s)
      池松夏紀, 坪井一人, 井上愛美, 清水嘉文, 宇山徹, 田中保, 上田夏生, 徳村彰
    • Organizer
      第50回日本薬学会日本薬剤師会日本病院薬剤師会中国四国支部学術大会
    • Place of Presentation
      香川
    • Year and Date
      2011-11-13
    • Related Report
      2011 Final Research Report
  • [Presentation] がん抑制遺伝子群HRASLSファミリーはリン脂質代謝酵素として機能する2011

    • Author(s)
      篠原尚樹, 宇山徹, 坪井一人, 芳地一, 上田夏生
    • Organizer
      第50回日本薬学会日本薬剤師会日本病院薬剤師会中国四国支部学術大会
    • Place of Presentation
      香川
    • Year and Date
      2011-11-13
    • Related Report
      2011 Final Research Report
  • [Presentation] マウス全脳におけるエタノールアミン含有脂質の分子種分析と関連酵素の基質特異性解析2011

    • Author(s)
      池松夏紀, ら
    • Organizer
      第50回日本薬学会日本薬剤師会日本病院薬剤師会中国四国支部学術大会
    • Place of Presentation
      サンポートホール高松かがわ国際会議場(香川県)
    • Year and Date
      2011-11-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] がん抑制遺伝子群HRASLSファミリーはリン脂質代謝酵素として機能する2011

    • Author(s)
      篠原尚樹, ら
    • Organizer
      第50回日本薬学会日本薬剤師会日本病院薬剤師会中国四国支部学術大会
    • Place of Presentation
      サンポートホール高松かがわ国際会議場(香川県)
    • Year and Date
      2011-11-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] レシチン・レチノール・アシルトランスフェラーゼにホモロジーを示すがん抑制遺伝子HRASLSファミリー・メンバーのリン脂質代謝酵素活性2011

    • Author(s)
      上田夏生, 宇山徹, 金星華, 坪井一人, 芳地一, 藤内武春
    • Organizer
      第332回脂溶性ビタミン総合研究委員会
    • Place of Presentation
      東京
    • Year and Date
      2011-09-30
    • Related Report
      2011 Final Research Report
  • [Presentation] レシチン・レチノール・アシルトランスフェラーゼにホモロジーを示すがん抑制遺伝子HRASLSファミリー・メンバーのリン脂質代謝酵素活性2011

    • Author(s)
      上田夏生, ら
    • Organizer
      第332回脂溶性ビタミン総合研究委員会
    • Place of Presentation
      昭和薬科大学(東京都)
    • Year and Date
      2011-09-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] N-アシル化プラズマローゲンリン脂質からのN-アシルエタノールアミンの生合成2011

    • Author(s)
      坪井一人, 岡本安雄, 池松夏紀, 井上愛美, 清水嘉文, 宇山徹, Dale G. Deutsch, 徳村彰, 上田夏生
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2011-09-22
    • Related Report
      2011 Final Research Report
  • [Presentation] Phospholipase A/ Acyltransferase(PLA/ AT)ファミリーの細胞内におけるN-アシルホスファチジルエタノールアミン生合成への関与2011

    • Author(s)
      宇山徹, 池松夏紀, 井上愛美, 篠原尚樹, 金星華, 坪井一人, 藤内武春, 徳村彰, 上田夏生
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2011-09-22
    • Related Report
      2011 Final Research Report
  • [Presentation] N-アシル化プラズマローゲンリン脂質からのN-アシルエタノールアミンの生合成2011

    • Author(s)
      坪井一人, ら
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Year and Date
      2011-09-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] Phospholipase A/Acyltransferase (PLA/AT)ファミリーの細胞内におけるN-アシルホスファチジルエタノールアミン生合成への関与2011

    • Author(s)
      宇山徹, ら
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Year and Date
      2011-09-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] The tumor suppressor A-C1 has an N-acyltransferase activity involved in NAPE formation2011

    • Author(s)
      Shinohara, N., Uyama, T., Jin, X.-H., Tsuboi, K., Tonai, T., Houchi, H., and Ueda, N
    • Organizer
      21st Annual symposium of the International Cannabinoid Research Society
    • Place of Presentation
      St. Charles, IL, USA
    • Year and Date
      2011-07-06
    • Related Report
      2011 Final Research Report
  • [Presentation] The tumor suppressor A-Cl has an N-acyltransferase activity involved in NAPE formation2011

    • Author(s)
      Naoki Shinohara, et al
    • Organizer
      21st Annual Symposium of the International Cannabinoid Research Society
    • Place of Presentation
      Pheasant Run (St.Charles, IL, USA)
    • Year and Date
      2011-07-06
    • Related Report
      2011 Annual Research Report
  • [Presentation] LRATに相同性を示す癌抑制遺伝子群HRASLSファミリーの脂質代謝酵素活性2011

    • Author(s)
      宇山徹, 篠原尚樹, 金星華, 坪井一人, 藤内武春, 芳地一, 上田夏生
    • Organizer
      日本ビタミン学会第63回大会
    • Place of Presentation
      広島
    • Year and Date
      2011-06-04
    • Related Report
      2011 Final Research Report
  • [Presentation] LRATに相同性を示す癌抑制遺伝子群HRASLSファミリーの脂質代謝酵素活性2011

    • Author(s)
      宇山徹, ら
    • Organizer
      日本ビタミン学会第63回大会
    • Place of Presentation
      安田女子大学(広島県)
    • Year and Date
      2011-06-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] 癌抑制遺伝子群HRASLSファミリー・メンバーHRASLS1のリン脂質代謝酵素活性2011

    • Author(s)
      宇山徹, 篠原尚樹, 金星華, 坪井一人, 藤内武春, 芳地一, 上田夏生
    • Organizer
      第53回日本脂質生化学会
    • Place of Presentation
      東京
    • Year and Date
      2011-05-12
    • Related Report
      2011 Final Research Report
  • [Presentation] 癌抑制遺伝子群HRASLSファミリー・メンバーHRASLS1のリン脂質代謝酵素活性2011

    • Author(s)
      宇山徹, ら
    • Organizer
      第53回日本脂質生化学会
    • Place of Presentation
      ホテル東京ガーデンパレス(東京都)
    • Year and Date
      2011-05-12
    • Related Report
      2011 Annual Research Report
  • [Presentation] LRATファミリーに属するヒト新規脂質代謝酵素群の機能解析2011

    • Author(s)
      宇山徹, 篠原尚樹, 金星華, 坪井一人, 藤内武春, 芳地一, 上田夏生
    • Organizer
      日本農芸化学会中四国支部第29回講演会日本ビタミン学会中国・四国地区第1回講演会合同講演会
    • Place of Presentation
      徳島
    • Year and Date
      2011-01-22
    • Related Report
      2011 Final Research Report
  • [Presentation] LRATファミリーに属するヒト新規脂質代謝酵素群の機能解析2011

    • Author(s)
      宇山徹, ら
    • Organizer
      日本農芸化学会中四国支部第29回講演会日本ビタミン学会中国・四国地区第1回講演会合同講演会
    • Place of Presentation
      徳島大学(徳島県)
    • Year and Date
      2011-01-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] 癌抑制遺伝子群HRASLSファミリー・メンバーA-C1の脂質代謝酵素としての解析2010

    • Author(s)
      篠原尚樹, 宇山徹, 金星華, 坪井一人, 藤内武春, 芳地一, 上田夏生
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      兵庫
    • Year and Date
      2010-12-09
    • Related Report
      2011 Final Research Report
  • [Presentation] ヒト癌抑制遺伝子HRASLSファミリーのリン脂質代謝酵素としての同定2010

    • Author(s)
      篠原尚樹, ら
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] Human tumor suppressors have a NAPE-forming N-acyltransferase activity2010

    • Author(s)
      Uyama, T., Jin, X.-H., Shinohara, N., Tsuboi, K., Tonai, T., and Ueda, N
    • Organizer
      20th Annual symposium of the International Cannabinoid Research Society
    • Place of Presentation
      Lund, Sweden
    • Year and Date
      2010-07-25
    • Related Report
      2011 Final Research Report
  • [Presentation] Human tumor suppressors have a NAPE-forming N-acyltransferase activity2010

    • Author(s)
      Uyama, T., et al.
    • Organizer
      20th Annual Symposium of the International Cannabinoid Research Society
    • Place of Presentation
      Scandic Star(ルンド,スウェーデン)
    • Year and Date
      2010-07-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒト癌抑制遺伝子HRASLSファミリーの脂質代謝酵素活性の解析2010

    • Author(s)
      宇山徹, 金星華, 篠原尚樹, 坪井一人, 上田夏生
    • Organizer
      第52回日本脂質生化学会
    • Place of Presentation
      群馬
    • Year and Date
      2010-06-14
    • Related Report
      2011 Final Research Report
  • [Presentation] ヒト癌抑制遺伝子HRASLSファミリーの脂質代謝酵素活性の解析2010

    • Author(s)
      宇山徹, ら
    • Organizer
      第52回日本脂質生化学会
    • Place of Presentation
      森秋旅館(群馬県)
    • Year and Date
      2010-06-14
    • Related Report
      2010 Annual Research Report
  • [Presentation] LRATファミリーに属する4種のヒト新規脂質代謝酵素の機能解析2010

    • Author(s)
      宇山徹, 金星華, 篠原尚樹, 坪井一人, 藤内武春, 上田夏生
    • Organizer
      日本ビタミン学会第62回大会
    • Place of Presentation
      岩手
    • Year and Date
      2010-06-12
    • Related Report
      2011 Final Research Report
  • [Presentation] LRATファミリーに属する4種のヒト新規脂質代謝酵素の機能解析2010

    • Author(s)
      宇山徹, ら
    • Organizer
      日本ビタミン学会第62回大会
    • Place of Presentation
      アイーナいわて県民情報交流センター(岩手県)
    • Year and Date
      2010-06-12
    • Related Report
      2010 Annual Research Report
  • [Presentation] Identification of the human tumor suppressors TIG3 and HRASLS2 as phospholipid-metabolizing enzymes2010

    • Author(s)
      Uyama, T., Jin, X.-H., Shinohara, N., Tsuboi, K., and Ueda, N
    • Organizer
      Keystone Symposia : Bioactive Lipids : Biochemistry and Diseases
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2010-06-09
    • Related Report
      2011 Final Research Report
  • [Presentation] Identification of the human tumor suppressors TIG3 and HRASLS2 as phospholipid-metabolizing enzymes2010

    • Author(s)
      Uyama, T., et al.
    • Organizer
      Keystone Symposia : Bioactive Lipids : Biochemistry and Diseases
    • Place of Presentation
      ウエスティン都ホテル京都(京都府)
    • Year and Date
      2010-06-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒト癌抑制遺伝子HRASLSファミリーのリン脂質代謝酵素としての同定2010

    • Author(s)
      宇山徹, 金星華, 篠原尚樹, 坪井一人, 上田夏生
    • Organizer
      第51回日本生化学会中国・四国支部例会
    • Place of Presentation
      山口
    • Year and Date
      2010-05-15
    • Related Report
      2011 Final Research Report
  • [Presentation] ヒト癌抑制遺伝子HRASLSファミリーのリン脂質代謝酵素としての同定2010

    • Author(s)
      宇山徹, ら
    • Organizer
      第51回日本生化学会中国・四国支部例会
    • Place of Presentation
      山口大学(山口県)
    • Year and Date
      2010-05-15
    • Related Report
      2010 Annual Research Report
  • [Remarks] ホームページ

    • URL

      http://www.kms.ac.jp/~biochem/index.html

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.kms.ac.jp/~biochem/index.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.kms.ac.jp/~biochem/index.html

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2016-04-21  

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