| Project/Area Number |
22790299
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | Tokai University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
ANDO Kiyoshi 東海大学, 医学部, 教授 (70176014)
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 再生医学 / 造血幹細胞 / 移植 / 酸化ストレス / DNA障害 / 再生医療 / ヒト化モデル動物 / DNA損傷 / 自己複製 / 幹細胞移植 / ヒト化モデルマウス |
|---|
| Research Abstract |
Stem cells of highly regenerative organs including blood are susceptible to endogenous DNA damage caused by both intrinsic and extrinsic stress. In this study, we demonstrate that the serial transplantation of human HSCs into immunodeficient mice triggers replication stress that induces incremental elevation of intracellular reactive oxygen species(ROS) levels and the accumulation of persistent DNA damage within the human HSCs. The study reveals that ROS play a causative role for DNA damage and the regulation of ROS have a major influence on human HSC aging.
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