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Molecular mechanism of neurite outgrowth regulated by PLCdelta3

Research Project

Project/Area Number 22790300
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionInstitute for Developmental Research, Aichi Human Service Center (2011)
Tokyo University of Pharmacy and Life Science (2010)

Principal Investigator

KOUCHI Zen  愛知県心身障害者コロニー発達障害研究所, 病理学部, 研究員 (70322485)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords細胞内シグナル伝達 / ホスホリパーゼC / 神経突起伸長 / 子宮内エレクトロポレーション / 小脳顆粒細胞 / RhoA / Rhoファミリー蛋白質 / 大脳皮質ニューロン
Research Abstract

Phospholipase Cδ3(PLCδ3), which is highly expressed in neuronal tissues, hydrolyzes phosphatidylinositol 4, 5-bisphosphate(PI(4, 5) P_2) and generates two second messengers, i. e. IP_3 and DAG. PLCδ3 knockdown in cerebral cortex of E14 embryo by in utero electroporation caused the migratory inhibition of cortical neurons in developing cerebral cortical plate. Furthermore, we demonstrated that neuronal outgrowth was inhibited in cerebral neurons, cerebellar granule cells, or Neuro2a cells when PLCδ3 was transiently knocked down during differentiation.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (3 results)

All 2011

All Journal Article (3 results) (of which Peer Reviewed: 3 results)

  • [Journal Article] Phospholipase Cδ3 regulates RhoA/Rho kinase signaling and neurite outgrowth2011

    • Author(s)
      Kouchi, Z., Igarashi, T., Shibayama, N., Inanobe, S., Sakurai, K., Yamaguchi, H., Fukuda, T., Yanagi, S., Nakamura, Y. and Fukami, K
    • Journal Title

      J. Biol. Chem

      Volume: 286 Issue: 10 Pages: 8459-8471

    • DOI

      10.1074/jbc.m110.171223

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phosphatidylinositol 5-phosphate 4-kinase type II beta is required for vitamin D receptor-dependent E-cadherin expression in SW480 cells2011

    • Author(s)
      Kouchi, Z., Fujiwara, Y., Yamaguchi, H., Nakamura, Y. and Fukami, K
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 408 Issue: 4 Pages: 523-529

    • DOI

      10.1016/j.bbrc.2011.04.045

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phospholipase Cδ3 regulates RhoA/Rho kinase Signaling and Neurite Outgrowth2011

    • Author(s)
      Kouchi, Z., et al.
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 286 Pages: 8459-8471

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed

URL: 

Published: 2010-08-23   Modified: 2016-04-21  

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