Molecular mechanism of neurite outgrowth regulated by PLCdelta3

• Project/Area Number: 22790300
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Institute for Developmental Research, Aichi Human Service Center (2011); Tokyo University of Pharmacy and Life Science (2010)
• Principal Investigator: KOUCHI Zen 愛知県心身障害者コロニー発達障害研究所, 病理学部, 研究員 (70322485)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 細胞内シグナル伝達 / ホスホリパーゼC / 神経突起伸長 / 子宮内エレクトロポレーション / 小脳顆粒細胞 / RhoA / Rhoファミリー蛋白質 / 大脳皮質ニューロン

Research Abstract

Phospholipase Cδ3(PLCδ3), which is highly expressed in neuronal tissues, hydrolyzes phosphatidylinositol 4, 5-bisphosphate(PI(4, 5) P_2) and generates two second messengers, i. e. IP_3 and DAG. PLCδ3 knockdown in cerebral cortex of E14 embryo by in utero electroporation caused the migratory inhibition of cortical neurons in developing cerebral cortical plate. Furthermore, we demonstrated that neuronal outgrowth was inhibited in cerebral neurons, cerebellar granule cells, or Neuro2a cells when PLCδ3 was transiently knocked down during differentiation.

Research Products

• [Journal Article] Phospholipase Cδ3 regulates RhoA/Rho kinase signaling and neurite outgrowth (2011)
  • Author(s): Kouchi, Z., Igarashi, T., Shibayama, N., Inanobe, S., Sakurai, K., Yamaguchi, H., Fukuda, T., Yanagi, S., Nakamura, Y. and Fukami, K
  • Journal Title: J. Biol. Chem Volume: 286 Issue: 10 Pages: 8459-8471
  • DOI: 10.1074/jbc.m110.171223
  • Peer Reviewed
• [Journal Article] Phosphatidylinositol 5-phosphate 4-kinase type II beta is required for vitamin D receptor-dependent E-cadherin expression in SW480 cells (2011)
  • Author(s): Kouchi, Z., Fujiwara, Y., Yamaguchi, H., Nakamura, Y. and Fukami, K
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 408 Issue: 4 Pages: 523-529
  • DOI: 10.1016/j.bbrc.2011.04.045
  • Peer Reviewed
• [Journal Article] Phospholipase Cδ3 regulates RhoA/Rho kinase Signaling and Neurite Outgrowth (2011)
  • Author(s): Kouchi, Z., et al.
  • Journal Title: The Journal of Biological Chemistry Volume: 286 Pages: 8459-8471
  • Peer Reviewed