| Project/Area Number |
22790316
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
SAKANE Hiroshi 大阪大学, 医学系・研究科, 招へい研究員 (80457291)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 分子病態学 / Wnt / Dkk1 / Glypican4 / LRP6 / エンドサイトーシス / 脂質ラフト |
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| Research Abstract |
We found that Wnt receptor LRP6 was phosphorylated in the lipid raft by Wnt3a stimulation. Wnt antagonist Dkk1 shifted endogenous LRP6 to the non-lipid raft fraction and induced clathrin-dependent endocytosis. We also found that glypican4, a member of proteoglycan, in the lipid raft activated theβ-catenin pathway. However, glypican4 in the non-lipid raft suppressedβ-catenin pathway but activatedβ-catenin-independent pathway. These results indicates how Wnt signaling pathway is regulated by Dkk1 and glypican4.
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