Roles of leukotriene receptor signaling in cell proliferation

• Project/Area Number: 22790320
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pathological medical chemistry
• Research Institution: Kyushu University
• Principal Investigator: SAEKI Kazuko 九州大学, 大学院・医学研究院, 助教 (00553273)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 生理活性脂質 / GPCR / 病態モデル

Research Abstract

BLT2 is a receptor for LTB_4 and 12-HHT and expressed on the epithelium of skin and intestine. To clarify the role of BLT2 in these tissues, the models of inflammatory colitis, tumorigenesis in the colon, and skin wound healing were performed. It was revealed that BLT2 suppresses inflammatory colitis and tumorigenesis in the colon and promotes skin wound healing.

Research Products

• [Journal Article] A high-affinity monoclonal antibody against the FLAG tag useful for G-protein coupled receptor study (2012)
  • Author(s): F.Sasaki
  • Journal Title: Analytical Biochemistry Volume: (掲載決定) Issue: 2 Pages: 157-165
  • DOI: 10.1016/j.ab.2012.03.014
  • Peer Reviewed
• [Journal Article] IL-27 inhibits pathophysiological intraocular neovascularization due to laser burn (2012)
  • Author(s): Hasegawa E, Oshima Y, Takeda A, Saeki K, Yoshida H, Sonoda KH, Ishibashi T
  • Journal Title: J.Leukoc.Biol. Volume: 91 Issue: 2 Pages: 267-273
  • DOI: 10.1189/jlb.1110603
  • Peer Reviewed
• [Journal Article] miR126 positively regulates mast cell proliferation and cytokine production through suppressing Spred1 (2011)
  • Author(s): Takuma Isguzaki, 他
  • Journal Title: Genes to Cells Volume: 16 Issue: 7 Pages: 803-814
  • DOI: 10.1111/j.1365-2443.2011.01529.x
  • Peer Reviewed
• [Journal Article] Raftlin is involved in the nucleocapture complex to induce poly(I:C)-mediated TLR3 activation. (2011)
  • Author(s): Watanabe A, Tatematsu M, Saeki K, Shibata S, Shime H, Yoshimura A, Obuse C, Seya T, Matsumoto M.
  • Journal Title: J. Biol. Chem. Volume: 286 Issue: 12 Pages: 10702-10711
  • DOI: 10.1074/jbc.m110.185793
  • Peer Reviewed
• [Journal Article] Leukotriene B_4 augments and restores FcgRs-dependent phagocytosis inmacrophages (2010)
  • Author(s): Okamoto F, Saeki K, Sumimoto H, Yamasaki S, and Yokomizo T
  • Journal Title: J. Biol. Chem Volume: 285 Issue: 52 Pages: 41113-41121
  • DOI: 10.1074/jbc.m110.175497
  • Peer Reviewed
• [Journal Article] Protective role of the leukotriene B4 receptor BLT2 in murine inflammatory colitis (2010)
  • Author(s): Iizuka, Y., Okuno, T., Saeki, K., Uozaki, H., Okada, S., Misaka, T., Sato, T., Toh, H., Fukayama, M.,Takeda, N., Kita, Y., Shimizu, T., Nakamura, M., and Yokomizo, T.
  • Journal Title: FASEB J Volume: 24 Issue: 12 Pages: 4678-4690
  • DOI: 10.1096/fj.10-165050
  • Peer Reviewed
• [Journal Article] HB-EGF function in cardiac valve development requires interaction with heparan sulfate proteoglycans (2010)
  • Author(s): Iwamoto R, Mine N, Kawaguchi T, Minami S, Saeki K, Mekada E
  • Journal Title: Development Volume: 137(13) Issue: 13 Pages: 2205-2214
  • DOI: 10.1242/dev.048926
  • Peer Reviewed
• [Journal Article] Attenuated Th1 induction by dendritic cells from mice deficient in the leukotriene B4 receptor 1 (2010)
  • Author(s): Toda A, Terawaki K, Yamazaki S, Saeki K, Shimizu T, Yokomizo T
  • Journal Title: Biochimie Volume: 92(6) Issue: 6 Pages: 682-691
  • DOI: 10.1016/j.biochi.2009.12.002
  • Peer Reviewed
• [Journal Article] Attenuated Th1 induction by dendritic cells from mice deficient in the leukotriene B4 receptor 1. (2010)
  • Author(s): Toda A, Terawaki K, Yamazaki S, Saeki K, Shimizu T, Yokomizo T
  • Journal Title: Biochimie Volume: 92(6) Pages: 682-691
  • Peer Reviewed
• [Journal Article] HB-EGF function in cardiac valve development requires interaction with heparan sulfate proteoglycans. (2010)
  • Author(s): Iwamoto R, Mine N, Kawaguchi T, Minami S, Saeki K, Mekada E
  • Journal Title: Development Volume: 137(13) Pages: 2205-2214
  • Peer Reviewed
• [Journal Article] Protective role of the leukotriene B4 receptor BLT2 in murine inflammatory colitis. (2010)
  • Author(s): Iizuka Y, Okuno T, Saeki K, Uozaki H, Okada S, Misaka T, Sato T, Toh H, Fukayama M, Takeda N, Kita Y, Shimizu T, Nakamura M, Yokomizo T
  • Journal Title: FASEB J Volume: 24(12) Pages: 4678-4690
  • Peer Reviewed
• [Journal Article] Leukotriene B4 augments and restores Fc gamma Rs-dependent phagocytosis in macrophages. (2010)
  • Author(s): Okamoto F, Saeki K, Sumimoto H, Yamasaki S, Yokomizo T
  • Journal Title: J Biol Chem Volume: 285(52) Pages: 41113-41121
  • Peer Reviewed
• [Journal Article] Raftlin is involved in the nucleocapture complex to induce poly(I:C)-mediated TLR3 activation.
  • Author(s): Watanabe A, Tatematsu M, Saeki K, Shibata S, Shime H, Yoshimura A, Obuse C, Seya T, Matsumoto M
  • Journal Title: J Biol Chem Volume: (in press)
  • Peer Reviewed
• [Presentation] BLT1 as a potential marker of mouse dendritic cell subset (2012)
  • Author(s): Koga T, Sasaki F, Saeki K, Okuno T, Yokomizo T
  • Organizer: The 9th Global COE International Symposium & 8th Young Investigators Forum
  • Place of Presentation: Fukuoka
  • Year and Date: 2012-01-21
• [Presentation] Crucial role of the leukotriene B4 receptor 2, BLT2 in epidermal wound healing (2011)
  • Author(s): Liu M, Saeki K, Yokomizo T
  • Organizer: The 10th Global COE International Symposium & 7th Young Investigators Forum
  • Place of Presentation: Singapore
  • Year and Date: 2011-12-23
• [Presentation] BLT1 as a potential marker of mouse dendritic cell subset (2011)
  • Author(s): Koga T, Sasaki F, Saeki K, Okuno T, Yokomizo T
  • Organizer: The 10th Global COE International Symposium & 7th Young Investigators Forum
  • Place of Presentation: Singapore
  • Year and Date: 2011-12-23
• [Presentation] BLT1 は樹状細胞の新規マーカー分子となりうるか? (2011)
  • Author(s): 古賀友紹, 佐伯和子, 佐々木文之, 奥野利明, 横溝岳彦
  • Organizer: 第8回東京呼吸器リサーチフォーラム
  • Place of Presentation: 東京
  • Year and Date: 2011-11-19
• [Presentation] Two distinct leukotriene B4 receptors: BLT1 and BLT2 (2011)
  • Author(s): Yokomizo T, Liu M, Saeki K, Okuno T
  • Organizer: The 10th JBS Biofrontier Symposium : New aspects of phospholipid biology and medicine
  • Place of Presentation: Fukuoka
  • Year and Date: 2011-11-16
• [Presentation] Leukotriene B4 augments and restores FcgRs-dependent phagocytosis in macrophages (2011)
  • Author(s): Saeki K, Okamoto F, Yokomizo T
  • Organizer: The 10th JBS Biofrontier Symposium : New aspects of phospholipid biology and medicine
  • Place of Presentation: Fukuoka
  • Year and Date: 2011-11-15
• [Presentation] Expression of an LTB4 receptor BLT1 in M2 macrophages (2011)
  • Author(s): Sasaki F, Koga T, Saeki K, Okuno T, Yokomizo T
  • Organizer: The 10th JBS Biofrontier Symposium : New aspects of phospholipid biology and medicine
  • Place of Presentation: Fukuoka
  • Year and Date: 2011-11-15
• [Presentation] BLT1 as a potential marker of mouse dendritic cell subsets (2011)
  • Author(s): Koga T, Sasaki F, Saeki K, Okuno T, Yokomizo T
  • Organizer: The 10th JBS Biofrontier Symposium : New aspects of phospholipid biology and medicine
  • Place of Presentation: Fukuoka
  • Year and Date: 2011-11-15
• [Presentation] Improved immunoprecipitation method for epitope-tagged GPCRs (2011)
  • Author(s): Morisaki Y, Sasaki F, Aratake Y, Okuno T, Saeki K, Yokomizo T
  • Organizer: The 10th JBS Biofrontier Symposium : New aspects of phospholipid biology and medicine
  • Place of Presentation: Fukuoka
  • Year and Date: 2011-11-15
• [Presentation] Leukotriene B_4 augments and restores FcγRs-dependent phagocytosis in macrophages (2011)
  • Author(s): Saeki K
  • Organizer: The 10th JBS Biofrontier Symposium : New aspects of phospholipid biology and medicine
  • Place of Presentation: 福岡
  • Year and Date: 2011-11-15
• [Presentation] Crucial role of the leukotriene B4 receptor BLT2 in epidermal wound healing (2011)
  • Author(s): Liu M., 佐伯和子, 奥野利明, 横溝岳彦
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-24
• [Presentation] 二つのロイコトリエンB4 受容体(BLT1, BLT2)のシグナルと病態生理機能 (2011)
  • Author(s): 佐伯和子, 劉〓, 岡元冬樹, 奥野利明, 横溝岳彦
  • Organizer: 第84回日本生化学会大会シンポジウム
  • Place of Presentation: 京都
  • Year and Date: 2011-09-23
• [Presentation] 二つのロイコトリエンB4受容体(BLT1,BLT2)のシグナルと病態生理機能 (2011)
  • Author(s): 佐伯和子
  • Organizer: 第84回日本生化学会大会:シンポジウム
  • Place of Presentation: 京都(招待講演)
  • Year and Date: 2011-09-23
• [Presentation] GPCR の免疫沈降に有用な新規抗FLAG 抗体の樹立と本抗体を用いた新規GPCR 結合タンパク質の探索 (2011)
  • Author(s): 佐々木文之, 奥野利明, 佐伯和子, 横溝岳彦
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-22
• [Presentation] Improved immunoprecipitation method for epitope-tagged GPCRs (2011)
  • Author(s): 森崎雄一, 佐々木文之, 荒武良総, 奥野利明, 佐伯和子, 横溝岳彦
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-22
• [Presentation] Crucial role of the 12-HHT receptor BLT2 in epidermal wound healin (2011)
  • Author(s): Liu M, Saeki K, Okuno T, Yokomizo T
  • Organizer: 第6回GCOR 理医連携リトリート
  • Place of Presentation: 阿蘇
  • Year and Date: 2011-08-05
• [Presentation] M2 マクロファージにおけるBLT1の発現と機能解析 (2011)
  • Author(s): 佐々木文之、佐伯和子、奥野利明、横溝岳彦
  • Organizer: 第6回GCOR 理医連携リトリート
  • Place of Presentation: 阿蘇
  • Year and Date: 2011-08-05
• [Presentation] BLT1 as a potential marker of mouse dendritic cell subsets (2011)
  • Author(s): Koga T, Sasaki F, Saeki K, Okuno T, Yokomizo T
  • Organizer: 第6回GCOR 理医連携リトリート
  • Place of Presentation: 阿蘇
  • Year and Date: 2011-08-05
• [Presentation] Improved immunpricipitation methods. for epitope-tagged GPCRs (2011)
  • Author(s): Morisaki Y, Sasaki F, Aratake Y, Okuno T, Saeki K, Yokomizo T
  • Organizer: 第6回GCOR 理医連携リトリート
  • Place of Presentation: 阿蘇
  • Year and Date: 2011-08-05
• [Presentation] Leukotriene B_4 augments and restores FcγRs-dependent phagocytosis in macrophages (2011)
  • Author(s): Saeki K, Okamoto F, Yamasaki S, Yokomizo T
  • Organizer: Experimental Biology 2011 (ASBMB Annual Meeting)
  • Place of Presentation: Washington, DC, USA
  • Year and Date: 2011-04-11
• [Presentation] Crucial role of the leukotriene B4 receptor BLT2 in epidermal wound healing (2011)
  • Author(s): Liu M, Saeki K, Abe Y, Yokomizo T
  • Organizer: Experimental Biology 2011 (ASBMB Annual Meeting)
  • Place of Presentation: Washington, DC, USA
  • Year and Date: 2011-04-10
• [Presentation] Generation of monoclonal antibodies specific for mouse leukotriene B4 receptor 1 and N-terminal flag sequence (2011)
  • Author(s): Sasaki F, Saeki K, Okuno T, Yokomizo T
  • Organizer: Experimental Biology 2011 (ASBMB Annual Meeting)
  • Place of Presentation: Washington, DC, USA
  • Year and Date: 2011-04-10
• [Presentation] Roles of 12-HHT/LTB4 receptor, BLT2 in murine inflammatory colitis (2011)
  • Author(s): Okuno T, Izuka Y, Saeki K, Yokomizo T
  • Organizer: Experimental Biology 2011(ASBMB Annual Meeting)
  • Place of Presentation: Washington, DC, USA
  • Year and Date: 2011-04-10
• [Presentation] Leukotriene B_4 augments and restores FcγRs-dependent phagocytosis in macrophages (2011)
  • Author(s): Saeki K
  • Organizer: Experimental Biology 2011 (ASBMB Annual Meeting)
  • Place of Presentation: アメリカワシントンDC
  • Year and Date: 2011-04-10
• [Presentation] Crucial role of the leukotriene B4 receptor 2, BLT2 in epidermal wound healing (2011)
  • Author(s): Liu M, Saeki K, Yokomizo T
  • Organizer: 6th G-COE Young Investigators Forum
  • Place of Presentation: Bintan Island, Indonesia
  • Year and Date: 2011-02-12
• [Presentation] Generation of monoclonal antibody for mouse leukotriene B4 receptor 1 (2011)
  • Author(s): Sasaki F, Saeki K, Okuno T, Yokomizo T
  • Organizer: 6th G-COE Young Investigators Forum
  • Place of Presentation: Bintan Island, Indonesia
  • Year and Date: 2011-02-12
• [Presentation] Crucial role of the leukotriene B4 receptor BLT2 in epidermal wound healing (2010)
  • Author(s): Liu M, Saeki K, Yokomizo T
  • Organizer: The 83^<th> Annual Meeting of the Japanese Biochemical Society
  • Place of Presentation: Kobe
  • Year and Date: 2010-12-08
• [Presentation] マクロファージ貪食におけるロイコトリエンB4 受容体(BLT1)の機能解析 (2010)
  • Author(s): 岡元冬樹、佐伯和子、奥野利明、山崎晶、横溝岳彦
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] Generation of monoclonal antibody for mouse leukotriene B4 receptor 1 (2010)
  • Author(s): 佐々木文之、佐伯和子、横溝岳彦
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] マウス炎症性腸炎における12-HHT/LTB4 受容体BLT2 の役割 (2010)
  • Author(s): 奥野利明、飯塚佳子、佐伯和子、清水孝雄、中村元直、横溝岳彦
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] 炎症性腸疾患モデルから明らかになった12-HHT/LTB4 受容体BLT2 の抗炎症作用 (2010)
  • Author(s): 奥野利明、飯塚佳子、佐伯和子、清水孝雄、中村元直、横溝岳彦
  • Organizer: 第7回東京リサーチフォーラム
  • Place of Presentation: 東京
  • Year and Date: 2010-11-06
• [Presentation] マクロファージ貪食におけるロイコトリエンB4 受容体(BLT1)の機能解析 (2010)
  • Author(s): 岡元冬樹、佐伯和子、奥野利明、山崎晶、横溝岳彦
  • Organizer: 第5回GCOE 理医連携リトリート
  • Place of Presentation: 福岡
  • Year and Date: 2010-08-18
• [Presentation] 抗マウスロイコトリエンB4 受容体モノクローナル抗体の作製とその有用性の評価 (2010)
  • Author(s): 佐々木文之、佐伯和子、奥野利明、横溝岳彦
  • Organizer: 第5回GCOE 理医連携リトリート
  • Place of Presentation: 福岡
  • Year and Date: 2010-08-18
• [Remarks]
  • URL: http://www.med.kyushu-u.ac.jp/biochem1/
• [Remarks]
  • URL: http://www.med.kyushu-u.ac.jp/biocheml/jiu_zhou_da_xue_yi_xue_bu_yi_hua_xue_fen_yenoHP/Top.html