Epigenetic transcriptional master regulator for hematopoietic cell differentiation

Research Project

Project/Area Number	22790335
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Human genetics
Research Institution	University of Occupational and Environmental Health, Japan
Principal Investigator	SUZUKI Mitsuhiro 産業医科大学, 医学部, 講師 (00321662)
Project Period (FY)	2010 – 2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords	血球分化 / 転写調節 / 発生・分化 / エピジェネティクス / 血球細胞
Research Abstract	Transcription factor PU. 1 is a hematopoietic master regulator and essential for the development of myeloid and B-cell lineages. PU. 1 functions in concert with other transcription factors and cofactors. As we shown previously represented, PU. 1 interact with both p300/cbp, which function as a coactivator and HDAC, which function as a corepressor. In this study, to determine whether modification of PU. 1 is responsible for switching its association between coactivaor and corepressor, we examined whether acetylation regulates the physical and functional activities of PU. 1.We found that PU. 1 was acetylated in vivo and its repressor activity was reduced when the putative acetylation motif in ETS domain were mutated. Our data suggest that acetylation might regulate the biological functions in erythroid cells.