Analysis of expression mechanisms of cell adhesion molecules via malignant neuroendocrine tumor cell-specific transcription factors

• Project/Area Number: 22790361
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Human pathology
• Research Institution: St. Marianna University School of Medicine
• Principal Investigator: SATO Hanako 聖マリアンナ医科大学, 医学部, 助教 (60438132)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: NeuroD / NCAM / プロモーター / 神経内分泌悪性腫瘍 / 肺癌 / 神経内分泌 / 悪性腫瘍 / 転写因子 / 細胞接着因子

Research Abstract

The author defined that neuroendocrine cell-specific basic helix-loop-helixtranscription factor, NeuroD, is directly involved in the expression of neural celladhesion molecule 1 (NCAM1) and that metastatic tumor-forming ability of lungcancer cells is accelerated by NCAM1 expression. These findings suggest thatneuroendorine cancer-specific differentiating phenotype is closely associated with itsrevealing high metastatic potential.

Research Products

• [Journal Article] Neural lineage-specific homeoprotein BRN2 is directly involved in TTF1 expression in small-cell lung cancer (2013)
  • Author(s): Sakaeda M, Sato H, Ishii J, Miyata C, Kamma H, Shishido-Hara Y, Shimoyamada H, Fujiwara M, Endo, T Tanaka R, Kondo H, Goya T, Aoki I, Yazawa T.
  • Journal Title: Lab Inv Volume: 93 Pages: 408-421
  • Peer Reviewed
• [Journal Article] POU domain transcription factor BRN2 is crucial for expression of ASCL1, ND1 and neuroendocrine marker molecules and cell growth in small cell lung cancer (2013)
  • Author(s): Ishii J, Sato H, Sakaeda M, Shishido-Hara Y, Hiramatsu C, Kamma H, Shimoyamada H, Fujiwara M, Endo T, Aoki I, Yazawa T.
  • Journal Title: Pathol Int Volume: 63 Pages: 158-168
  • Peer Reviewed
• [Journal Article] POU domain transcription factor BRN2 is crucial for expression of ASCL1, ND1 and neuroendocrine marker molecules and cell growth in small cell lung cancer (2013)
  • Author(s): Ishii J, Sato H, et al.
  • Journal Title: Pathology International Volume: 63 Issue: 3 Pages: 158-168
  • DOI: 10.1111/pin.12042
  • Peer Reviewed
• [Journal Article] Neural lineage-specific homeoprotein BRN2 is directly involved in TTF1 expression in small-cell lung cancer (2013)
  • Author(s): Sakaeda M, Sato H, et al.
  • Journal Title: Laboratory Investigation Volume: 93 Issue: 4 Pages: 408-421
  • DOI: 10.1038/labinvest.2013.2
  • Peer Reviewed
• [Journal Article] Differences of molecular expression mechanisms among neural cell adhesion molecule 1, synaptophysin, and chromogranin A in lung cancer cells (2012)
  • Author(s): Kashiwagi K, Ishii J, Sakaeda M, Arimasu Y, Shimoyamada H, Sato H, Miyata C, Kamma H, Aoki I, Yazawa T
  • Journal Title: Pathol Int Volume: 62 Pages: 232-245
  • NAID: 10031185195
  • Peer Reviewed
• [Journal Article] Differences of molecular expression mechanisms among neural cell adhesion molecule 1, synaptophysin, and chrmogranin A in lung cancer cells (2012)
  • Author(s): Kashiwagi K, Ishii J, Sakaeda M, Arimasu Y, Shimoyamada H, Sato H, Miyata C, Kamma H, Aoki I, Yazawa T
  • Journal Title: Pathol Int Volume: VOL.62 Issue: 4 Pages: 232-245
  • DOI: 10.1111/j.1440-1827.2011.02781.x
  • Peer Reviewed
• [Journal Article] Insulin-like growth factor binding protein-4 gene silencing in lung adenocarcinomas (2011)
  • Author(s): Sato H, Sakaeda M, Ishii J, Kashiwagi K, Shimoyamada H, Okudela K, Tajiri N, Ohmori T, Ogura T, Woo T, Masuda M, Hirata K, Kitamura H, Yazawa T
  • Journal Title: Pathol Int Volume: 61 Pages: 19-27
  • NAID: 10031185042
  • Peer Reviewed
• [Journal Article] Keratinocyte growth factor gene transduction ameliorates pulmonarys induced by bleomycin in mice (2011)
  • Author(s): Sakamoto S, et al
  • Journal Title: Am J Respir Cell Mol Biol Volume: VOL.45 Pages: 489-497
  • Peer Reviewed
• [Journal Article] Insulin-like growth factor binding protein-4 gene silencing in lung adenocarcinomas. (2011)
  • Author(s): Sato H, et al.
  • Journal Title: Pathology International Volume: 61 Pages: 19-27
  • NAID: 10031185042
  • Peer Reviewed
• [Journal Article] Keratinocyte growth factor gene transduction ameliorates pulmonary fibrosis induced by bleomycin in mice (2010)
  • Author(s): Sakamoto S, Yazawa T, Baba Y, Sato H, Kanegae Y, Saito I, Goto T Kurahashi K
  • Journal Title: Am J Respir Cell Biol Volume: 45 Pages: 489-497
  • Peer Reviewed
• [Journal Article] Early growth response-1 induces and enhances vascular endothelial growth factor-a expression in lung cancer cells (2010)
  • Author(s): Shimoyamada H, Yazawa T, Sato H, Okudela K, Ishii J, Sakaeda M, Kashiwagi K, Suzuki T, Mitsui H, Woo T, Tajiri M, Ohmori T, Ogura T, Masuda M, Oshiro H, Kitamura H.
  • Journal Title: Am J Pathol Volume: 177 Pages: 70-83
  • Peer Reviewed
• [Journal Article] Early growth response-1 induces and enhances vascular endothelial growth factor-A expression in lung cancer cells. (2010)
  • Author(s): Shimoyamada H, et al.
  • Journal Title: American Journal of Pathology Volume: 177 Pages: 70-83
  • Peer Reviewed
• [Presentation] 肺小細胞癌におけるTTF-1発現はPOUドメイン転写因子BRN2により直接的に制御されている。 (2013)
  • Author(s): 榮田昌史、佐藤華子、石井順、宮田千恵、菅間博、宍戸-原由紀子、下山田博明、藤原正親、遠藤哲哉、青木一郎、矢澤卓也。
  • Organizer: 第102回日本病理学会総会
  • Place of Presentation: ロイトン札幌
  • Year and Date: 2013-06-07
• [Presentation] POU型転写因子BRN2は肺小細胞癌細胞の神経/神経内分泌形質発現および増殖活性に関与する。 (2013)
  • Author(s): 石井順、佐藤華子、榮田昌史、宍戸-原由紀子、平松千恵、菅間博、下山田博明、藤原正親、遠藤哲哉、青木一郎、矢澤卓也。
  • Organizer: 第102回日本病理学会総会
  • Place of Presentation: ロイトン札幌
  • Year and Date: 2013-06-07
• [Presentation] 肺癌におけるThyroidtranscriptionfactor-1の発現機序に関与する因子の検索 (2012)
  • Author(s): 榮田昌史、佐藤華子、宮田千恵、石井順、有益優、柏木維人、下山田博明、菅間博、青木一郎、矢澤卓也。
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 京王プラザホテル,東京
  • Year and Date: 2012-04-27
• [Presentation] 肺癌の神経/神経内分泌形質に ASCL1 およびREST が与える影響の解析 (2012)
  • Author(s): 石井順、有益優、榮田昌史、柏木維人、下山田博明、佐藤華子、宮田千恵、藤原正親、菅間博、青木一郎、矢澤卓也。
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 京王プラザホテル,東京
  • Year and Date: 2012-04-27
• [Presentation] 肺癌におけるThyroid transcription factor-1の発現機序に関与する因子の検索 (2012)
  • Author(s): 榮田昌史、佐藤華子、他
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 京王プラザホテル(東京)
• [Presentation] 肺癌の神経・神経内分泌形質にASCL1およびRESTが与える影響の解析 (2012)
  • Author(s): 石井順、佐藤華子、他
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 京王プラザホテル(東京)
• [Presentation] 神経内分泌マーカー発現に対するNeuroD、ASCL1の関与について (2011)
  • Author(s): 柏木維人、石井順、榮田昌史、有益優、佐藤華子、奥寺康司、下山田博明、北村均、矢澤卓也
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: パシフィコ横浜,横浜
  • Year and Date: 2011-04-28
• [Presentation] 神経内分泌マーカー発現に対するNeuroD,ASCL1の関与について (2011)
  • Author(s): 柏木維人, 他
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-04-28
• [Presentation] IGFBP-4expressiondeficiencybyepigeneticsilencinginnon-smallcelllungcancers (2010)
  • Author(s): 佐藤華子、榮田昌史、石井順、柏木維人、奥寺康司、下山田博明、北村均、矢澤卓也
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 京王プラザホテル,東京
  • Year and Date: 2010-04-28
• [Presentation] 肺癌細胞におけるNeuroD発現はNeuralcelladheasionmolecule1(NCAM1)を誘導する (2010)
  • Author(s): 柏木維人、石井順、榮田昌史、佐藤華子、奥寺康司、下山田博明、北村均、矢澤卓也
  • Organizer: 第99回日本病理学会総会。
  • Place of Presentation: 京王プラザホテル,東京
  • Year and Date: 2010-04-28
• [Presentation] IGFBP-4 expression deficiency by epigenetic silencing in non-small cell lung cancers. (2010)
  • Author(s): 佐藤華子, 他
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: (京王プラザホテル)東京
  • Year and Date: 2010-04-27
• [Presentation] 肺癌細胞におけるNeuroD発現はNeural Cell Adhesion Molecule 1 (NCAM1)を誘導する (2010)
  • Author(s): 柏木維人, 他
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: (京王プラザホテル)東京
  • Year and Date: 2010-04-27