Analysis of CTGF,the new candidate of the tumor suppressor gene in ovarian cancer, for personalized anti-cancer therapy

• Project/Area Number: 22790364
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Human pathology
• Research Institution: National Defense Medical College
• Principal Investigator: KIKUCHI Ryoko 防衛医科大学校, 医学教育部医学科専門課程, 助教 (80535063)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 分子病理 / 卵巣癌 / CTGF / 抗癌剤感受性 / 低酸素 / 蛍光標識二次元電気泳動法 / CTGF遺伝子 / 抗癌剤 / 化学療法の個別化

Research Abstract

Knockdown of CTGF with specific small interfering RNAs in OVISE cells, the ovarian clear cell carcinoma cell line, and in OVSAHO cells, the ovarian serous carcinoma cell line enhanced resistance to apoptosis on exposure to paclitaxel, but not enhanced resistance to apotosis on exposure to carboplatin, cisplatin and doxorubicin.

Research Products

• [Journal Article] Stathmin is involved in the cooperativeeffect of Zoledronic acid and gefitinib onbone homing breast cancer cells in vitro (2012)
  • Author(s): Oda M, Iwaya K, Kikuchi R, Kobayashi T,Yoneda T, Nishikawa K, Matsubara O, KohnoN
  • Journal Title: Journal of Bone Oncology Volume: Vol. 1 Pages: 40-46
  • Peer Reviewed
• [Journal Article] Proteomeanalysis of ovarian cancer cell lines underhypoxic condition by two-dimensionaldifference gel electrophoresis (2D-DIGE)[abstract] (2012)
  • Author(s): Kikuchi R, Iwaya K, Matsubara O
  • Journal Title: Proceedings of the 103rdAnnual Meeting of the American Associationfor Cancer Research Research; 2012 Mar 31-Apr 4;Chicago, Illinois. Philadelphia (PA): AACR Pages: 462-462
  • Peer Reviewed
• [Journal Article] Stathmin is involved in the cooperative effect of Zoledronic acid and gefitinib on bone homing breast cancer cells in vitro. (2012)
  • Author(s): Oda M, Iwaya K, Kikuchi R, Kobayashi T, Yoneda T, Nishikawa K, Matsubara O, Kohno N
  • Journal Title: Journal of Bone Oncology Volume: 1 Pages: 40-46
  • Peer Reviewed
• [Journal Article] Proteome analysis of ovarian cancer cell lines under hypoxic condition by two-dimensional difference gel electrophoresis (2D-DIGE) [abstract]. (2012)
  • Author(s): Kikuchi R, Iwaya K, Matsubara O
  • Journal Title: In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research Volume: 1 Pages: 462-462
  • Peer Reviewed
• [Journal Article] Weekly administrationof temsirolimus for heavily pretreatedpatients with clear cell carcinoma of theovary: a report of six cases (2011)
  • Author(s): Takano M, Kikuchi Y, Kudoh K, Goto T,Furuya K, Kikuchi R, Kita T, Fujiwara K,Shiozawa T, Aoki D
  • Journal Title: International Journal of ClinicalOncology Volume: Vol. 16 Pages: 605-609
  • Peer Reviewed
• [Journal Article] Effects of bevacizumaband pegylated liposomal doxorubicin for thepatients with recurrent or refractoryovarian cancers (2011)
  • Author(s): Kudoh K, Takano M, Kouta H, Kikuchi R,Kita T, Miyamoto M, Watanabe A, Kato M,Goto T, Kikuchi Y
  • Journal Title: Gynecologic Oncology Volume: Vol.122 Pages: 233-237
  • Peer Reviewed
• [Presentation] アレイCGH解析による卵巣癌関連遺伝子の同定と分子細胞診断への応用 (2012)
  • Author(s): 菊池良子、井本逸勢、嘉屋隆介、三宅清彦、茂木真、坂本優、田中忠夫、岡本愛光、松原修、稲澤譲治
  • Organizer: 日本臨床細胞学会
  • Place of Presentation: 新潟
• [Presentation] 乳癌の骨転移に関与するstathminの過剰発現と抗腫瘍効果との関連 (2012)
  • Author(s): 小田美規、岩屋啓一、菊池良子、山田公人、海瀬博史、松原修、河野範男
  • Organizer: 第20回日本乳癌学会学術総会
  • Place of Presentation: 熊本
• [Presentation] 低酸素暴露下の卵巣癌細胞株のプロテオーム解析 (2012)
  • Author(s): 菊池良子、岩屋啓一、松原修
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 東京
• [Presentation] 乳癌の骨転移に関与するstathmin の過剰発現と抗腫瘍効果との関連 (2012)
  • Author(s): 小田美規, 岩屋啓一, 菊池良子, 山田公人, 海瀬博史, 松原修, 河野範男
  • Organizer: 第20 回日本乳癌学会学術総会
  • Place of Presentation: 熊本
• [Presentation] 低酸素暴露下の卵巣癌細胞株のプロテオーム解析 (2012)
  • Author(s): 菊池良子, 岩屋啓一, 松原修
  • Organizer: 第101 回日本病理学会総会
  • Place of Presentation: 東京