Analysis of Dickkopf molecule in Ewing's sarcoma

• Project/Area Number: 22790366
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Human pathology
• Research Institution: National Research Institute for Child Health and Development
• Principal Investigator: OKITA Hajime 独立行政法人国立成育医療研究センター, 小児血液・腫瘍研究部, 室長 (50317260)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 骨 / 軟部 / 肉腫 / Wnt / 小児 / WNT / Ewing肉腫

Research Abstract

DKK1 suppress nuclear accumulation ofβ-catenin induced by Wnt3a in serum starved Ewing's sarcoma cells and it is suggested that the DKK1 inhibit classical WNT signal in these cells. Although DKK1 suppress tumor formation in xenotranplatation model of Ewing's sarcoma, DKK1 added in the standard cell culture environment seems not to affect tumor formation nor proliferation. Tumor formation might be suppressed in vivo via mechanisms, like suppression of angiogenesis.

Research Products

• [Journal Article] Ewing肉腫 (2011)
  • Author(s): 陳基明,牧本敦,横山良平,角美奈子,尾崎敏文,石井猛,川井章,山田健志,田地野崇宏,石田剛,大喜多肇,川本博,浅見恵子,麦島秀雄
  • Journal Title: 小児外科 Volume: 43(11) Pages: 1229-1233
• [Journal Article] A microfabricated scaffold induces the spheroid formation of human bone marrow-derived mesenchymal progenitor cells and promotes efficient adipogenic differentiation (2011)
  • Author(s): Miyagawa Y, Okita H, Hiroyama M, Sakamoto R, Kobayashi M, Nakajima H, Katagiri YU, Fujimoto J, Hata JI, Umezawa A, Kiyokawa N.
  • Journal Title: Tissue Eng Part A Volume: 17 Pages: 513-21
  • Peer Reviewed
• [Journal Article] A microfabricated scaffold induces the spheroid formation of human bone marrow-derived mesenchymal progenitor cells and promotes efficient adipogenic differentiation. (2011)
  • Author(s): Miyagawa Y, Okita H, Hiroyama M, Sakamoto R, Kobayashi M, Nakajima H, Katagiri YU, Fujimoto J, Hata JI, Umezawa A, Kiyokawa N.
  • Journal Title: Tissue Eng Part A Volume: 17 Pages: 513-21
  • Peer Reviewed
• [Journal Article] Ewing肉腫の病理診断上の問題点 (2010)
  • Author(s): 石田剛,大喜多肇,長谷川匡,秦順一
  • Journal Title: 日本整形外科学会雑誌 Volume: 84 Pages: 1126-1131
  • NAID: 10027708525
• [Presentation] ユーイング肉腫ファミリー腫瘍におけるEWS/ETS融合遺伝子の標的遺伝子DKK1/DKK2の腫瘍形成における役割 (2012)
  • Author(s): 大喜多肇,秋元信吾,宮川世志幸,近森穣,藤本純一郎,小林健一郎,秦順一,清河信敬
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 東京
  • Year and Date: 2012-04-26
• [Presentation] 小児腎肉腫におけるDNAメチル化解析 (2011)
  • Author(s): 上野瞳,大喜多肇,清河信敬
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-14
• [Presentation] ユーイング肉腫ファミリー腫瘍におけるDickkopfファミリー分子の発現制御とその意義 (2011)
  • Author(s): 大喜多肇,近森穣,宮川世志幸,秋元信吾,小林健一郎,藤本純一郎,秦順一,清河信敬
  • Organizer: 第53回日本小児血液・がん学会学術集会
  • Place of Presentation: 前橋
  • Year and Date: 2011-11-26
• [Presentation] Dietary bioflavonoids induce apoptosis in Ewing sarcoma cells (2011)
  • Author(s): Okita H, Iijima K, Fujimoto J, Kiyokawa N
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-05