Functional analysis of Trem-like 4 in mice

• Project/Area Number: 22790376
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Experimental pathology
• Research Institution: Osaka University (2011); The Institute of Physical and Chemical Research (2010)
• Principal Investigator: HEMMI Hiroaki 大阪大学, 免疫学フロンティア研究センター, 寄附研究部門助教 (20451924)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 樹状細胞 / マクロファージ / イムノグロブリンスーパーファミリー / サブセット / 受容体

Research Abstract

Triggering Receptor Expressed on Myeloid cells(Trem) family is characterized by an immunoglobulin-like domain at extracellular domain and short cytoplasmic tail. In this study, we aimed to clarify physiological functions of a Trem family member, Trem-like 4(Treml4). We could found a candidate molecule of Treml4 ligand. In addition, we analyzed Treml4-deficent mice. In Treml4-deficient mice, uptake of dying cells and cross-presentation of cell-associated antigens in vivo was similarly observed to that in littermate control mice, suggesting that Treml4 is not involved in these responses. We also found that an engineered anti-Treml4 antibody conjugated with antigens can efficiently induce T cell responses.

Research Products

• [Journal Article] Treml4, an Ig Superfamily Member, Mediates Presentation of Several Antigens to T Cells In Vivo, Including Protective Immunity to HER2 Protein (2012)
  • Author(s): Hiroaki Hemmi, Neeha Zaidi, Bei Wang, Ines Matos, Christopher Fiorese, Ashira Lubkin, Lori Zbytnuik, Koji Suda, Kenneth Zhang, Masaki Noda, Tsuneyasu Kaisho, Ralph M. Steinman, and Juliana Idoyaga
  • Journal Title: J Immunol Volume: 188 Issue: 3 Pages: 1147-1155
  • DOI: 10.4049/jimmunol.1102541
  • Peer Reviewed
• [Journal Article] CD8陽性樹状細胞サブセットの動的制御機構の解析 (2012)
  • Author(s): 邊見弘明, 山崎千尋, 星野克明, 改正恒康
  • Journal Title: Cytometry Research Volume: 22 Pages: 31-35
  • NAID: 10030199789
  • URL: http://www.cytometry.jp/scientist/journals/%E3%83%90%E3%83%83%E3%82%AF%E3%83%8A%E3%83%B3%E3%83%90%E3%83%BC
  • Peer Reviewed
• [Journal Article] Conservation of a chemokine system, XCR1 and its ligand, XCL1, between human and mice (2010)
  • Author(s): Yamazaki, C., Miyamoto, R., Hoshino, K., Fukuda, Y., Sasaki, I., Saito, M., Ishiguchi, H., Yano, T., Sugiyama, T., Hemmi, H., Tanaka, T., Hamada, E., Hirashima, T., Amakawa, R., Fukuhara, S., Nomura, S., Ito, T., and Kaisho, T.
  • Journal Title: Biochem Biophys Res Commun Volume: 397 Issue: 4 Pages: 756-761
  • DOI: 10.1016/j.bbrc.2010.06.029
  • Peer Reviewed
• [Journal Article] Conservation of a chemokine system, XCR1 and its ligand, XCL1, between human and mice. (2010)
  • Author(s): Chihiro Yamazaki, et al.
  • Journal Title: Biochemical and Biophysical Research Communication Volume: 397 Pages: 756-761
  • Peer Reviewed
• [Presentation] ケモカイン受容体Xcr1発現樹状細胞のin vivoにおける機能的意義 (2011)
  • Author(s): 山崎千尋, 邊見弘明, 宮本理恵, 佐々木泉, 伊藤量基, 星野克明, 改正恒康
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ
  • Year and Date: 2011-11-28
• [Presentation] ケモカイン受容体Xcr1発現樹状細胞のin vivoにおける機能的意義 (2011)
  • Author(s): 山崎千尋, 他
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ(千葉県)
  • Year and Date: 2011-11-28
• [Presentation] CD8陽性樹状細胞の動的制御機構の解析 (2011)
  • Author(s): 邊見弘明, 山崎千尋, 星野克明, 改正恒康
  • Organizer: 第21回日本サイトメトリー学会学術集会
  • Place of Presentation: 京都市国際会館
  • Year and Date: 2011-06-26
• [Presentation] CD8陽性樹状細胞の動的制御機構の解析 (2011)
  • Author(s): 邉見弘明, 他
  • Organizer: 第21回日本サイトメトリー学会学術集会
  • Place of Presentation: 京都市国際交流会館(京都府)
  • Year and Date: 2011-06-26