The investigation of the association between variants of the base excision repair(BER) enzymes and carcinogenesis, and the repair activities of BER enzymes against etheno-DNA adducts
Project/Area Number |
22790378
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Experimental pathology
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Research Institution | Hamamatsu University School of Medicine |
Principal Investigator |
GOTO Masanori 浜松医科大学, 医学部, 特別研究員 (00432203)
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Project Period (FY) |
2010 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 8-ヒドロキシグアニン / MUTYH / エテノアダクト / 塩基除去修復遺伝子 / 大腸がん / 活性酸素種 / 過酸化脂質 / 塩基除去修復酵素 / TDG / 塩基除去修復 / 胃がん |
Research Abstract |
We successfully prepared highly homogeneous human MUTYH recombinant protein, and compared the repair activity of 14 variant proteins with that of the wild -type protein. Several variant proteins showed striking low activity, and their variants may contribute to the development of MUTYH-associated polyposis. To search new substrate of base excision repair enzymes, we prepared 8 enzymes, and examined the repair activity against an oligonucleotide containing etheno-DNA adducts, a lipid peroxidation-derived DNA adducts. The base excision repair enzyme, TDG, had activity that is capable of removing thymine mispaired with etheno-DNA adduct of cytosine or guanine.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Lipid peroxidation-induced DNA adducts in human gastric mucosa2012
Author(s)
Matsuda T, Tao H, Goto M, Yamada H, Suzuki M, Wu Y, Xiao N, He Q, Guo W, Cai Z, Kurabe N, Ishino K, Matsushima Y, Shinmura K, Konno H, Maekawa M, Wang Y, Sugimura H
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Journal Title
Carcinogenesis
Volume: 34
Issue: 1
Pages: 121-127
DOI
Related Report
Peer Reviewed
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[Journal Article] Genetic susceptibility to lung cancer2011
Author(s)
H.Sugimura, H.Tao, M.Suzuki, H.Mori, M.Tsuboi, S.Matsuura, M.Goto, K.Shimnura, T.Ozawa, F.Tanioka, N.Sato, Yoshitaka Matsushima, S.Kageyama, K.Funai, P.-H.Chou, T.Matsuda
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Journal Title
Front.Biosci.(Schol.Ed.)
Volume: S3
Issue: 1
Pages: 1463-1477
DOI
Related Report
Peer Reviewed
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[Journal Article] Aberrant expression and mutation-inducing activity of AID in human lung cancer2011
Author(s)
Shinmura K, Igarashi H, Goto M, Tao H, Yamada H, Matsuura S, Tajima M, Matsuda T, Yamane A, Funai K, Tanahashi M, Niwa H, Ogawa H, Sugimura H
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Journal Title
Ann Surg Oncol
Volume: 18
Issue: 7
Pages: 2084-2092
DOI
Related Report
Peer Reviewed
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[Presentation] Germline alterations o the CDH1 gene in familial gastric cancer in the Japaese population2012
Author(s)
Yamada H, Shinmura K, Ito H, Kasami H, Sasaki N, Shima H, Tao H, Goto M, Du C, Tsuneyoshi T, Tanioka F, Sugimura H
Organizer
71th Annual Meeting of the Japanese Cancer Association
Place of Presentation
Sapporo
Related Report
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[Presentation] Absence of germline mono-allelic promoter hypermethylation of the CDH1 gene in gastric cancer patients2010
Author(s)
Yamada H, Shinmura K, Mori H, Goto M, Iwaizumi M, Konno H, Kataoka H,Yamada M, Ozawa T, Tsuneyoshi T, Tanioka F, Sugimura H
Organizer
69th Annual Meeting of the Japanese Cancer Association
Place of Presentation
Osaka
Related Report
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