Production ofgene-modified animalbytransposase-mediated gene transduction into spermatogonial stem cells.

• Project/Area Number: 22790379
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Experimental pathology
• Research Institution: Kyoto University
• Principal Investigator: TAKASHIMA Seiji 京都大学, 大学院・医学研究科, 助教 (40396891)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 疾患モデル動物 / トランスポゾン / 精子幹細胞

Research Abstract

For the production of gene-modified animal, germline stem cells were established fromDBA/2 mice and various lineages of rats. Germline stem cells derived from C57BL/6mice were also established by using C166 endothelial cells as a feeder. To assesswhether PiggyBac-mediated gene transduction could be applicable to germline stemcells, EGFP expression vector was transduced into germline stem cells via PiggyBacsystem. As a result, PiggyBac mediated transduction could produce stabletransfectants that expressed strong EGFP, suggesting that transposase-mediated genetransduction is effective for germline stem cells.

Research Products

• [Journal Article] Rac mediates mouse spermatogonial stem cell homing to germline niches by regulating transmigration through the blood-testis barrier (2011)
  • Author(s): Takashima, S, . Kanatsu-Shinohara, M., Tanaka, T., Takehashi, M., Morimoto, H., Shinohara, T
  • Journal Title: Cell Stem Cell Volume: 9 Issue: 5 Pages: 463-475
  • DOI: 10.1016/j.stem.2011.08.011
  • Peer Reviewed
• [Journal Article] Dynamic changes in EPCAM expression during spermatogonial stem cell differentiation in the mouse testis (2011)
  • Author(s): 篠原美都、高島誠司、石井慧、篠原隆司
  • Journal Title: PLoS One Volume: 6 Issue: 8 Pages: e23663-e23663
  • DOI: 10.1371/journal.pone.0023663
  • NAID: 120003405532
  • Peer Reviewed
• [Journal Article] Regulation of germline transmission in the male mice by p21 and p27 cyclin-dependent kinase inhibitors (2010)
  • Author(s): Kanatsu-Shinohara, M., Takashima, S. and Shinohara T
  • Journal Title: Proc. Natl. Acad. Sci Volume: 107(14) Issue: 14 Pages: 6210-6215
  • DOI: 10.1073/pnas.0914448107
  • Peer Reviewed
• [Journal Article] Genetic Influences in Mouse Spermatogonial Stem Cell Self-Renewal (2010)
  • Author(s): Kanatsu-Shinohara, M., Ogonuki, N., Miki, H., Inoue, K., Morimoto, H., Takashima, S., Ogura A., Shinohara, T.
  • Journal Title: Journal of Reproduction and Development Volume: 56 Issue: 1 Pages: 145-153
  • DOI: 10.1262/jrd.09-153N
  • NAID: 10026323942
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed
• [Journal Article] Transmission distortion by loss of p21 or p27 cyclin-dependent kinase inhibitors following competitive spermatogonial transplantation. (2010)
  • Author(s): Kanatsu-Shinohara M, Takashima S, Shinohara T.
  • Journal Title: Proceeding of National Academy of Science U.S.A. Volume: 107 Pages: 6205-6210
  • Peer Reviewed
• [Remarks]
  • URL: http://www.med.kyoto-u.ac.jp/J/grad_school/introduction/1504/
• [Remarks]
  • URL: http://www.med.kyoto-u.ac.jp/J/grad_school/introduction/1504/
• [Remarks]
  • URL: http://www.med.kyoto-u.ac.jp/J/grad_school/introduction/1504/