Production ofgene-modified animalbytransposase-mediated gene transduction into spermatogonial stem cells.
Project/Area Number |
22790379
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Experimental pathology
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Research Institution | Kyoto University |
Principal Investigator |
TAKASHIMA Seiji 京都大学, 大学院・医学研究科, 助教 (40396891)
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Project Period (FY) |
2010 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 疾患モデル動物 / トランスポゾン / 精子幹細胞 |
Research Abstract |
For the production of gene-modified animal, germline stem cells were established fromDBA/2 mice and various lineages of rats. Germline stem cells derived from C57BL/6mice were also established by using C166 endothelial cells as a feeder. To assesswhether PiggyBac-mediated gene transduction could be applicable to germline stemcells, EGFP expression vector was transduced into germline stem cells via PiggyBacsystem. As a result, PiggyBac mediated transduction could produce stabletransfectants that expressed strong EGFP, suggesting that transposase-mediated genetransduction is effective for germline stem cells.
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Report
(3 results)
Research Products
(8 results)
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[Journal Article] Rac mediates mouse spermatogonial stem cell homing to germline niches by regulating transmigration through the blood-testis barrier2011
Author(s)
Takashima, S, . Kanatsu-Shinohara, M., Tanaka, T., Takehashi, M., Morimoto, H., Shinohara, T
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Journal Title
Cell Stem Cell
Volume: 9
Issue: 5
Pages: 463-475
DOI
Related Report
Peer Reviewed
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[Journal Article] Genetic Influences in Mouse Spermatogonial Stem Cell Self-Renewal2010
Author(s)
Kanatsu-Shinohara, M., Ogonuki, N., Miki, H., Inoue, K., Morimoto, H., Takashima, S., Ogura A., Shinohara, T.
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Journal Title
Journal of Reproduction and Development
Volume: 56
Issue: 1
Pages: 145-153
DOI
NAID
ISSN
0916-8818, 1348-4400
Related Report
Peer Reviewed
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