| Project/Area Number |
22790393
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
KUMAGAI Takashi 東京医科歯科大学, 大学院・医歯学総合研究科, 助教 (40369054)
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| Co-Investigator(Renkei-kenkyūsha) |
OHTA Nobuo 東京医科歯科大学, 医歯学総合研究科, 教授 (10143611)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | 蠕虫 / RNAi / 住血吸虫 / sid-1 / SID-1 / dsRNA / 寄生蠕虫 / 日本住血吸虫 / RNA干渉 |
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| Research Abstract |
Schistosomes induce the RNA interference(RNAi) using long dsRNA in soaking method, such as C. elegans. It is previously reported that schistosomes incorporate the dsRNA, including host derived RNA. To understand the relationships between RNA uptake and parasitism, sid-1 gene, related to RNAi machinery, was investigated. The mRNA of sid-1 was increased in the larval stage after the invasion to the host, and was regulated by the induction of long dsRNA. However, the schistosomes knocked down with sid-1 siRNA, were not effects to RNAi machinery, dsRNA uptake, and parasitism in the host. These mean that schistosomes may have the distinct RNAi machinery.
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