The host RNA uptake related gene in Schistosoma japonicum

• Project/Area Number: 22790393
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: KUMAGAI Takashi 東京医科歯科大学, 大学院・医歯学総合研究科, 助教 (40369054)
• Co-Investigator(Renkei-kenkyūsha): OHTA Nobuo 東京医科歯科大学, 医歯学総合研究科, 教授 (10143611)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: 蠕虫 / RNAi / 住血吸虫 / sid-1 / SID-1 / dsRNA / 寄生蠕虫 / 日本住血吸虫 / RNA干渉

Research Abstract

Schistosomes induce the RNA interference(RNAi) using long dsRNA in soaking method, such as C. elegans. It is previously reported that schistosomes incorporate the dsRNA, including host derived RNA. To understand the relationships between RNA uptake and parasitism, sid-1 gene, related to RNAi machinery, was investigated. The mRNA of sid-1 was increased in the larval stage after the invasion to the host, and was regulated by the induction of long dsRNA. However, the schistosomes knocked down with sid-1 siRNA, were not effects to RNAi machinery, dsRNA uptake, and parasitism in the host. These mean that schistosomes may have the distinct RNAi machinery.

Research Products

• [Journal Article] Phylogenetic relationships ofrat lungworm, Angiostrongylus cantonensis, isolated from different geographical regions revealed widespread multiple lineages (2012)
  • Author(s): Tokiwa T, Harunari T, Tanikawa T, Komatsu N, Koizumi N, Tung KC, Suzuki J, Kadosaka T, Takada N, Kumagai T, Akao N, Ohta N
  • Journal Title: Parasitol Int Volume: (in press)
• [Journal Article] Loop-mediated isothermal amplification(LAMP): early detection of Toxoplasma gondii infection in mice (2012)
  • Author(s): Kong QM, Lu SH, Tong QB, Lou D, Chen R, Zheng B, Kumagai T, Wen LY, Ohta N, Zhou XN.
  • Journal Title: Parasit Vectors Volume: 3
• [Journal Article] Interleukin-4(IL-4) and IL-13 suppress excessive neutrophil infiltration and hepatocyte damage during acute murine schistosomiasis japonica (2012)
  • Author(s): Seki T, Kumagai T, Kwansa-Bentum B, Furushima-Shimogawara R, Anyan WK, Miyazawa Y, Iwakura Y, Ohta N
  • Journal Title: Infect Immun Volume: 80 Pages: 159-68
• [Journal Article] Interleukin-4 (IL-4) and IL-13 suppress excessive neutrophil infiltration and hepatocyte damage during acute murine schistosomiasis japonica (2012)
  • Author(s): T.Seki
  • Journal Title: Infect Immun Volume: 80 Issue: 1 Pages: 159-168
  • DOI: 10.1128/iai.05581-11
  • Peer Reviewed
• [Journal Article] Loop-mediated isothermal amplification (LAMP) : early detection of Toxoplasma gondii infection in mice (2012)
  • Author(s): Kong QM, Lu SH, Tong QB, Lou D, Chen R, Zheng B, Kumagai T, Wen LY, Ohta N, Zhou XN
  • Journal Title: Parasites and Vectors Volume: 5 Issue: 1 Pages: 2-2
  • DOI: 10.1186/1756-3305-5-2
  • Peer Reviewed
• [Journal Article] Phylogenetic relationships of rat lungworm, Angiostrongylus cantonensis, isolated from different geographical regions revealed widespread multiple lineages (2012)
  • Author(s): Tokiwa T, et al
  • Journal Title: Parasitol Int Volume: (in press) Issue: 3 Pages: 431-6
  • DOI: 10.1016/j.parint.2012.02.005
  • Peer Reviewed
• [Journal Article] Cerebral Schistosomiasis Due to Schistosoma haematobium Confirmed by PCR Analysis of Brain Specimen (2011)
  • Author(s): Imai K, Koibuchi T, Kumagai T, Maeda T, Osada Y, Ohta N, Koga M, Nakamura H, Miura T, Iwamoto A, Fujii T
  • Journal Title: J Clin Microbiol Volume: 49 Pages: 3703-6
• [Journal Article] Plasmodium falciparum isolates from southern Ghana exhibit polymorphisms in the SERCA-type PfATPase6 though sensitive to artesunate in vitro (2011)
  • Author(s): Kwansa-Bentum B, Ayi I, Suzuki T, Otchere J, Kumagai T, Anyan WK, Osei JH, Asahi H, Ofori MF, Akao N, Wilson MD, Boakye DA, Ohta N.
  • Journal Title: Malar J Volume: 11
  • Peer Reviewed
• [Journal Article] Administrative practices of health professionals and use of artesunate-amodiaquine by community members for treating uncomplicated malaria in southern Ghana : implications for artemisinin-based combination therapy deployment (2011)
  • Author(s): Kwansa-Bentum B, Ayi I, Suzuki T, Otchere J, Kumagai T, Anyan WK, Asahi H, Akao N, Wilson MD, Boakye DA, Ohta N
  • Journal Title: Trop Med Int Health Volume: 10 Pages: 134-139
  • Peer Reviewed
• [Journal Article] Schistosomicidal and antifecundity effects of oral treatment of synthetic endoperoxide N-89 (2011)
  • Author(s): Taniguchi T, Kumagai T, Shimogawara R, Ichinose S, Hiramoto A, Sato A, Morita M, Nojima M, Kim HS, Wataya Y, Ohta N.
  • Journal Title: Parasitol Int Volume: 60 Pages: 231-6
  • Peer Reviewed
• [Journal Article] Schistosomicidal and anti-fecundity effects of oral treatment of synthetic endoperoxide compound N-89 (2011)
  • Author(s): Taniguchi T, Kumagai T, Shimogawara R, Ichinose S, Hiramoto A, Sato A, Morita M, Nojima M, Kim HS, Wataya Y, Ohta N
  • Journal Title: Parasitology International Volume: 60 Issue: 3 Pages: 231-236
  • DOI: 10.1016/j.parint.2011.02.007
  • Peer Reviewed
• [Journal Article] Administrative practices of health professionals and use of artesunate-a modiaquine by community members for treating uncomplicated malaria in southern Ghana : implications for artemisinin-based combination therapy deployment (2011)
  • Author(s): Kwansa-Bentum B, et al
  • Journal Title: Trop Med Int Health Volume: 16(10) Issue: 10 Pages: 1215-1224
  • DOI: 10.1111/j.1365-3156.2011.02833.x
  • Peer Reviewed
• [Journal Article] Plasmodium falciparum isolates from southern Ghana exhibit polymorphisms in the SERCA-type PfATPase6 though sensitive to artesunate in vitro (2011)
  • Author(s): 鈴木高史(Kwansa-Bentum Bethel)
  • Journal Title: Malaria Journal Volume: 11 Issue: 1 Pages: 187-187
  • DOI: 10.1186/1475-2875-10-187
  • Peer Reviewed
• [Journal Article] Cerebral schistosomiasis due to Schistosoma haematobium confirmed by PCR analysis of brain specimen (2011)
  • Author(s): Imai K, Koibuchi T, Kumagai T, Maeda T, Osada Y, Ohta N, Koga M, Nakamura H, Miura T, Iwamoto A, Fujii T
  • Journal Title: Journal of Clinical Microbiology Volume: 49 Issue: 10 Pages: 3703-6
  • DOI: 10.1128/jcm.01073-11
  • Peer Reviewed
• [Journal Article] Schistosomicidal and antifecundity effects of oral treatment of synthetic endoperoxide N-89. (2011)
  • Author(s): Taniguchi T, Kumagai T, Shimogawara R, Ichinose S, Hiramoto A, Sato A, Morita M, Nojima M, Kim HS, Wataya Y, Ohta N
  • Journal Title: Parasitol Int Volume: (in press)
  • Peer Reviewed
• [Journal Article] Detection of early and single infections of Schistosoma japonicum in the intermediate host snail, Oncomelania hupensis, by PCR and loop-mediated isothermal amplification(LAMP) assay (2010)
  • Author(s): Kumagai T, Furushima-Shimogawara R, Ohmae H, Wang TP, Lu S, Chen R, Wen L, Ohta N.
  • Journal Title: Am J Trop Med Hyg Volume: 83 Pages: 542-8
  • Peer Reviewed
• [Journal Article] Loop-mediated isothermal amplification assay for detection and discrimination of Toxocara canis and T. cati eggs directly from sand samples (2010)
  • Author(s): Macuhova K, Kumagai T, Akao N, Ohta N.
  • Journal Title: J. Parasitol. Volume: 96 Pages: 1224-7
  • Peer Reviewed
• [Journal Article] Schistosome eggs have a direct role in the induction of basophils capable of a high level of IL-4 production : Comparative study of single-and bisexual infection of Schistosoma mansoni in vivo (2010)
  • Author(s): Anyan KW, Kumagai T, Shimogawara FR, Seki T, Akao N, Obata K, Bentum KB, Bosompen MK, Boakye AD, Wilson DM, Karasuyama H, Ohta N
  • Journal Title: Tropical Medicine and Health Volume: 38 Pages: 13-22
  • NAID: 10026420055
  • Peer Reviewed
• [Journal Article] Detection of early and single infections of Schistosoma japonicum in the intermediate host snail, Oncomelania hupensis, by PCR and loop-mediated isothermal amplification (LAMP) assay. (2010)
  • Author(s): Kumagai T, Furushima-Shimogawara R, Ohmae H, Wang TP, Lu S, Chen R, Wen L, Ohta N
  • Journal Title: Am J Trop Med Hyg. Volume: 83(3) Pages: 542-548
  • Peer Reviewed
• [Journal Article] Loop-mediated isothermal amplification assay for detection and discrimination of Toxocara canis and Toxocara cati eggs directly from sand samples. (2010)
  • Author(s): Macuhova K, Kumagai T, Akao N, Ohta N
  • Journal Title: J Parasitol Volume: 96(6) Pages: 1224-1227
  • Peer Reviewed
• [Presentation] 日本住血吸虫成虫より分泌されるエクソソーム様小胞の免疫抑制作用 (2012)
  • Author(s): 熊谷貴ら
  • Organizer: 第81回日本寄生虫学会大会
  • Place of Presentation: 西宮
• [Presentation] Rapid mRNA degradation by soaking method of antisense RNA, but not dsRNA in Schistosoma japonicum (2012)
  • Author(s): Kumagai T, et al
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo
• [Presentation] 日本住血吸虫RNAi関連分子SID-1のmRNAヴァリアントの機能 (2011)
  • Author(s): 熊谷貴, ら
  • Organizer: 第80回日本寄生虫学会大会
  • Place of Presentation: 東京
  • Year and Date: 2011-03-30
• [Presentation] 日本住血吸虫感染貝モニタリングを目指したLAMP法の適用 (2011)
  • Author(s): 熊谷貴ら
  • Organizer: 第52回日本熱帯医学会大会・第26回日本国際保険医療学会学術大会
  • Place of Presentation: 東京
• [Presentation] 日本住血吸虫でみられたアンチセンスRNAによるRNA干渉 (2011)
  • Author(s): 熊谷貴ら
  • Organizer: 第22回日本生体防御学会学術総会
  • Place of Presentation: 那覇
• [Presentation] 日本住血吸虫RNAi関連分子SID-1のmRNAヴァリアントの機 (2011)
  • Author(s): 熊谷貴ら
  • Organizer: 第80回日本寄生虫学会大会
  • Place of Presentation: 東京
• [Presentation] The application of PCR and loop-mediated isothermal amplification(LAMP) methods to the field surveillance for snail infections of Schistosoma japonicum (2011)
  • Author(s): Kumagai, et al
  • Organizer: The 59th ASTMH Annual Meeting
  • Place of Presentation: Atlanta
• [Presentation] Characterization of systemic RNAi deficiency-1 from Schistosoma japonicum and the mechanisms of dsRNA incorporation (2011)
  • Author(s): Kumagai, et al
  • Organizer: The 12th International Congress of Parasitology
  • Place of Presentation: Melbourne
• [Presentation] Rapid mRNA degradation by soaking method of antisense RNA, but not dsRNA in Schistosoma japonicum (2011)
  • Author(s): Kumagai T, et al
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌
• [Presentation] The application of PCR and loop-mediated isothermal amplification (LAMP) methods to the field surveillance for snail infections of Schistosoma japonicum (2010)
  • Author(s): Kumagai, et al
  • Organizer: The 59^<th> ASTMH Annual Meeting
  • Place of Presentation: アトランタ
  • Year and Date: 2010-11-04
• [Presentation] Characterization of systemic RNAi deficiency-1 from Schistosoma japonicum and the mechanisms of dsRNA incorporation (2010)
  • Author(s): Kumagai, et al
  • Organizer: The 12^<th> International Congress of Parasitology
  • Place of Presentation: メルボルン
  • Year and Date: 2010-08-19
• [Presentation] 日本住血吸虫のSID-1とスカベンジャー受容体はそれぞれ異なった場所でRNA干渉に関わる (2010)
  • Author(s): 熊谷貴ら
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] イメージング解析による日本住血吸虫の宿主RNA取り込み機構の解析 (2010)
  • Author(s): 熊谷貴ら
  • Organizer: 第79回日本寄生虫学会大会
  • Place of Presentation: 旭川
• [Presentation] 日本住血吸虫のSID-1とスカベンジャー受容体はそれぞれ異なった場所でRNA干渉に関わる (2010)
  • Author(s): 熊谷貴, ら
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会・合同大会
  • Place of Presentation: 神戸