Analysis of the mechanism of virulence factor dependent-inflammasome activation upon Listeria monocytogenes infection

• Project/Area Number: 22790405
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: Kyoto University
• Principal Investigator: HARA Hideki 京都大学, 医学研究科, 助教 (30456892)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 感染免疫 / Listeria monocytogenes / Listeriolysin O / Inflammasome

Research Abstract

Inflammasome activation is responsible for the programmed cell death pytoptosis and cytokine production including IL-1 beta and IL-18. Based on our results, it was clarified that AIM2, type I IFN and kinase signals such as Syk are involved in the inflammasome activation induced by Listeria monocytogenes. Furthermore, we narrowed down the responsible region of listeriolysin O for inflammasome activation as a bacterial factor and revealed that the amino acid region from 204 to 254 of listeriolysin O is important for the activity.

Research Products

• [Journal Article] HTLV-1 bZIP factor impairs cell-mediated immunity by suppressing production of Th1 cytokines (2012)
  • Author(s): Sugata K, Satou Y, Yasunaga J, Hara H, Ohshima K, Utsunomiya A, Mitsuyama M, Matsuoka M.
  • Journal Title: Blood Volume: 119 Pages: 434-444
  • NAID: 120003752111
  • URL: http://bloodjournal.hematologylibrary.org/content/119/2/434.long
  • Peer Reviewed
• [Journal Article] Critical roles of ASC inflammasomes in caspase-1 activation and host innate resistance to Streptococcus pneumoniae infection (2011)
  • Author(s): Fang R, Tsuchiya K, Kawamura I, Shen Y, Hara H, Sakai S, Yamamoto T, Fernandes-Alnemri T, Yang R, Hernandez-Cuellar E, Dewamitta SR, Xu Y, Qu H, Alnemri ES, Mitsuyama M.
  • Journal Title: The Journal of Immunology Volume: 187 Pages: 4890-4899
  • URL: http://www.jimmunol.org/content/187/9/4890.long
  • Peer Reviewed
• [Journal Article] Expression of the Mycobacterium tuberculosis PPE37 protein in Mycobacterium smegmatis induces low tumour necrosis factor alpha and interleukin 6 production in murine macrophages (2011)
  • Author(s): Daim S, Kawamura I, Tsuchiya K, Hara H, Kurenuma T, Shen Y, Dewamitta SR, SakaiS, Nomura T, Qu H, Mitsuyama M.
  • Journal Title: Journal of Medical Microbiology Volume: 60 Pages: 582-591
  • URL: http://jmm.sgmjournals.org/content/60/5/582.full
  • Peer Reviewed
• [Journal Article] Cellular vacuolation induced by Clostridium perfringens epsilon-toxin (2011)
  • Author(s): Masahiro Nagahama
  • Journal Title: FEBS J. Volume: 278 Issue: 18 Pages: 3395-3407
  • DOI: 10.1111/j.1742-4658.2011.08263.x
  • Peer Reviewed
• [Journal Article] Involvement of absent in melanoma 2 in inflammasome activation in macrophages in fected with Listeria monocytogenes (2010)
  • Author(s): Tsuchiya K, Hara H, Kawamura I, Nomura T, Yamamoto T, Daim S, Dewamitta SR, Shen Y, Fang R, Mitsuyama M.
  • Journal Title: The Journal of Immunology Pages: 1186-1192
  • URL: http://www.jimmunol.org/content/185/2/1186.long
  • Peer Reviewed
• [Journal Article] Toll-like receptor 2-and MyD88-dependent phosphatidylinositol 3-kinase and Rac1 activation facilitates the phagocytosis of Listeria monocytogenes by murine macrophages (2010)
  • Author(s): Shen Y, Kawamura I, Nomura T, Tsuchiya K, Hara H, Dewamitta SR, Sakai S, Qu H, Daim S, Yamamoto T, Mitsuyama M.
  • Journal Title: Infection and Immunity Volume: 78 Pages: 2857-2867
  • NAID: 120002381103
  • URL: http://iai.asm.org/content/78/6/2857.long
  • Peer Reviewed
• [Journal Article] Listeriolysin O-dependent bacterial entry into cytoplasm is required for calpain activation and IL-1alpha secretion in macrophages infected with Listeria monocytogenes (2010)
  • Author(s): Dewamitta SR, Nomura T, Kawamura I, Hara H, Tsuchiya K, Kurenuma T, Shen Y, Daim S, Yamamoto T, Qu H, Sakai S, Xu Y, Mitsuyama M
  • Journal Title: Infection and Immunity Volume: 78 Pages: 1884-1894
  • URL: http://iai.asm.org/content/78/5/1884.long
  • Peer Reviewed
• [Journal Article] Involvement of absent in melanoma 2 in inflammasome activation in macrophages infected with Listeria monocytogenes. (2010)
  • Author(s): Tsuchiya K, Hara H, Kawamura I, Nomura T, Yamamoto T, Daim S, Dewamitta SR, Shen Y, Fang R, Mitsuyama M.
  • Journal Title: The Journal of Immunology Volume: 185 Pages: 1186-1195
  • Peer Reviewed
• [Journal Article] Toll-like receptor 2- and MyD88-dependent phosphatidylinositol 3-kinase and Rac1 activation facilitates the phagocytosis of Listeria monocytogenes by murine macrophages. (2010)
  • Author(s): Shen Y, Kawamura I, Nomura T, Tsuchiya K, Hara H, Dewamitta SR, Sakai S, Qu H, Daim S, Yamamoto T, Mitsuyama M.
  • Journal Title: Infection and Immunity Volume: 78 Pages: 2857-2867
  • NAID: 120002381103
  • Peer Reviewed
• [Presentation] Analysis of the region responsible for inflammasome activation in listeriolysin O (2012)
  • Author(s): Hara H.
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
  • Year and Date: 2012-03-27
• [Presentation] Analysis of the region responsible for inflammasome activation in listeriolysin O (2012)
  • Author(s): Hara H, Igwilo A, Tsuchiya K, Yamamoto T, Eduardo HC, Kawamura I, Mitsuyama M
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎ブリックホール(長崎県)(招待講演)
  • Year and Date: 2012-03-27
• [Presentation] Involvement of listeriolysin O-mediated inflammasome activation in bacterial virulence upon Listeria monocytogenes infection (2011)
  • Author(s): Hara H.
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張
  • Year and Date: 2011-11-28
• [Presentation] Involvement of listeriolysin O mediated inflammasome activation in bacterial virulence upon Listeria monocytogenes infection (2011)
  • Author(s): Hara H, Tsuchiya K, Yamamoto T, Kawaraura I, Mitsuyama M
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ(千葉県)
  • Year and Date: 2011-11-28
• [Presentation] リステリア感染における主要病原因子listeriolysin Oのインフラマソーム形成を介した病原性への関与 (2011)
  • Author(s): 原英樹
  • Organizer: 第64回日本細菌学会関西支部総会
  • Place of Presentation: 大阪
  • Year and Date: 2011-11-19
• [Presentation] リステリア感染における主要病原因子listeriolysin Oのインフラマソーム形成を介した病原性への関与 (2011)
  • Author(s): 原英樹、土屋晃介、山本武司、河村伊久雄、光山正雄
  • Organizer: 第64回日本細菌学会関西支部総会
  • Place of Presentation: 大阪府立大学(大阪府)
  • Year and Date: 2011-11-19
• [Presentation] Participation of inflammasome activation mediated by listeriolysin O in bacterial virulence upon Listeria monocytogenes infection (2011)
  • Author(s): Hara H.
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo
  • Year and Date: 2011-09-08
• [Presentation] Participation of inflammasome activation mediated by listeriolysin O in bacterial virulence upon Listeria monocytogenes infection (2011)
  • Author(s): Hara H, Tsuchiya K, Yamamoto T, Kawamura I, Mitsuyama M
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2011-09-08
• [Presentation] Involvement of type I interferon in inflammasome formation in macrophages infected with Listeria monocytogenes (2010)
  • Author(s): Hara H.
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: Kobe
  • Year and Date: 2010-08-22
• [Presentation] Involvement of type I interferon in inflammasome formation in macrophages infected with Listeria monocytogenes (2010)
  • Author(s): Hara H, Tsuchiya K, Kawamura I, Yamamoto T, Nomura T, Mitsuyama M.
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2010-08-22
• [Presentation] リステリア感染で誘導されるcaspase-1活性化におけるlisteriolysin Oの重要性 (2010)
  • Author(s): 原英樹
  • Organizer: 第57回トキシンシンポジウム
  • Place of Presentation: 長浜
  • Year and Date: 2010-07-14
• [Presentation] リステリア感染で誘導されるcaspase-1活性化におけるlisteriolysin Oの重要性 (2010)
  • Author(s): 原英樹
  • Organizer: 第57回トキシンシンポジウム
  • Place of Presentation: 長浜ロイヤルホテル
  • Year and Date: 2010-07-14
• [Remarks] ホームページ
  • URL: http://www.med.kyoto-u.ac.jp/J/grad_school/introduction/1204/
• [Remarks]
  • URL: http://www.med.kyoto-u.ac.jp/J/grad_school/introduction/1204/