Analysis of Vibrio parahaemolyticus T6SS apparatus and its ATPase

• Project/Area Number: 22790406
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: Osaka University
• Principal Investigator: AKEDA Yukihiro 大阪大学, 微生物病研究所, 特任講師 (60444527)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 腸炎ビブリオ / タンパク質分泌機構 / ATPase / 6型タンパク質分泌装置

Research Abstract

In this project, we analysed the structure of type 6 secretion system apparatus and its ATPase. Purification of T6SS associated ATPase was done and measured its activity, and also identified the proteins interacting with T6SS-specific secreted protein and its functional domains.

Research Products

• [Journal Article] Role of platelet apoptosis and apoptotic platelet clearance in thrombocytopenia in secondary dengue virus infections (2012)
  • Author(s): Alonzo, M., Lacuesta, T., Dimaano, EM., Kurosu, T., Suarez, LC., Mapua, CA Akeda, Y., Matias, RR., Kuter, DJ., Nagata, S., Natividad, FF. and Oishi, K.
  • Journal Title: The Journal of Infectious Diseases Volume: 205 Pages: 1321-1329
  • DOI: 10.1093/infdis/jis180
  • Peer Reviewed
• [Journal Article] Population-Based Study of Streptococcus suis Infection in Humans in Phayao Province in Northern Thailand. (2012)
  • Author(s): Takeuchi D, Kerdsin A, Pienpringam A, Loetthong P, Samerchea S, Pakkinee Loetthong P, Khamisra K, Wongwan N, Areeratana P, Chiranairadul P, Lertchayanti S, Petcharat S, Yowang A, Chaiwongsaen P, Nakayama T, Akeda Y, Hamada S, Sawanpanyalert P, Dejsirilert
  • Journal Title: PLoS One Volume: 7(2)
  • DOI: 10.1371/journal.pone.0031265
  • Peer Reviewed
• [Journal Article] 肺炎球菌ワクチン (2012)
  • Author(s): 明田幸宏、大石和徳
  • Journal Title: 診断と治療 Volume: 100 Pages: 455-458
• [Journal Article] Recombinant AexU effector protein of Aeromonas veronii bv. sobria disrupts the actin cytoskeleton by downregulation of Rac1 and induces direct cytotoxicity to β4-integrin expressing cell lines. (2011)
  • Author(s): Said Kamal Abolghait, Tetsuya Iida, Toshio Kodama, Vlademir V. Cantarelli, Yukihiro Akeda and Takeshi Honda
  • Journal Title: Microb Pathog Volume: 51 Pages: 454-465
  • DOI: 10.1016/j.micpath.2011.09.006
  • Peer Reviewed
• [Journal Article] VopV, an F-Actin-Binding Type III secretion effector, is Responsible for Vibrio parahaemolyticus-Induced enterotoxicity. (2011)
  • Author(s): Hirotaka Hiyoshi, Toshio Kodama, Kazunobu Saito, Kazuyoshi Gotoh, Shigeaki Matsuda, Yukihiro Akeda, Takeshi Honda and Tetsuya Iida
  • Journal Title: Cell Host Microbe Volume: 10 Pages: 401-409
  • DOI: 10.1016/j.chom.2011.08.014
  • Peer Reviewed
• [Journal Article] Identification of the Vibrio parahaemolyticus Type III Secretion System 2-Associated Chaperone VocC for the T3SS2-specific effector VopC. (2011)
  • Author(s): Yukihiro Akeda, Toshio Kodama, Kazunobu Saito, Tetsuya Iida, Kazunori Oishi and Takeshi Honda
  • Journal Title: FEMS Microbiol Lett Volume: 324 Pages: 156-164
  • DOI: 10.1111/j.1574-6968.2011.02399.x
  • Peer Reviewed
• [Journal Article] Sepsis and Spontaneous Bacterial Peritonitis in Thailand (2011)
  • Author(s): Kerdsin A, Dejsirilert S, Sawanpanyalert P, Boonnark A, Noithachang W, Sriyakun D, Simkum S, Chokngam S, Gottschalk M, Akeda Y, Oishi K
  • Journal Title: Lancet Volume: 378 Pages: 960-960
  • DOI: 10.1016/s0140-6736(11)60923-9
  • Peer Reviewed
• [Journal Article] Genotypic profile of Streptococcus suis serotype 2 and clinical features of infection in humans (2011)
  • Author(s): Kerdsin A, Dejsirilert S, Puangpatra P, Sripakdee S, Chumla K, Boonkerd N, Polwichai P, Tanimura S, Takeuchi D, Nakayama T, Nakamura S, Akeda Y, Gottschalk M, Sawanpanyalert P, Oishi K
  • Journal Title: Thailand. Emerg Infect Dis Volume: 17 Pages: 835-842
  • DOI: 10.3201/eid1705.100754
  • Peer Reviewed
• [Journal Article] Comparative Effects of Toll-Like Receptor Agonists on a Low Dose PspA Intranasal Vaccine against Fatal Pneumococcal Pneumonia in Mice (2011)
  • Author(s): Zhenyu Piao, Keita Oma, Hirokazu Ezoe, Yukihiro Akeda, Kazunori Tomono and Kazunori Oishi
  • Journal Title: Journal of Vaccines and Vaccination Volume: 2 Pages: 113-113
  • DOI: 10.4172/2157-7560.1000113
  • Peer Reviewed
• [Journal Article] Intranasal vaccination with pneumococcal surface protein A plus poly(I : C) protects against secondary pneumococcal pneumonia in mice (2011)
  • Author(s): Hirokazu Ezoe, Yukihiro Akeda, Taiki Aoshi, , Zenyo Piao, Shohei Koyama, Takeshi Tanimoto, Ken J Ishii and Kazunori Oishi
  • Journal Title: Vaccine Volume: 29 Pages: 1754-1761
  • DOI: 10.1016/j.vaccine.2010.12.117
  • Peer Reviewed
• [Journal Article] Streptococcus suis infection induces to bacterial accumulation in the kidney (2011)
  • Author(s): Tatsuya Nakayama, Dan Takeuchi, Yukihiro Akeda, Kazunori Oishi
  • Journal Title: Microbial Pathogenesis Volume: 50 Pages: 87-93
  • DOI: 10.1016/j.micpath.2010.11.005
  • Peer Reviewed
• [Journal Article] Intranasal vaccination with pneumococcal surface protein A plus poly(I:C) protects against secondary pneumococcal pneumonia in mice (2011)
  • Author(s): H Ezoe, Y Akeda, T Aoshi, Z Piao, S Koyama, T Tanimoto, KJ Ishii, K Oishi
  • Journal Title: Vaccine Volume: 29 Pages: 1754-1761
  • Peer Reviewed
• [Journal Article] Clinical features of Streptococcus suis serotype 2 infections in relation to the genotypic profiles of isolates in Thailand. (2011)
  • Author(s): A Kerdsin, S Deisirilert, P Puangpatra, S Sripakdee, K Chumla, N Boonkerd, P Polwichai, S Tanimura, D Takeuchi, T Nakayama, S Nakamura, Y Akeda, P Sawanpanyalert, K Oishi
  • Journal Title: Emerging Infectious Diseases Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Bile acid-induced virulence gene expression of Vibrio parahaemolyticus reveals a novel therapeutic potential for bile acid sequestrants (2010)
  • Author(s): Kazuyoshi Goto, Toshio Kodama, Hirotaka Hiyoshi, Kaori Izutsu, Kwon-Sam Park, Rikard Dryselius, Yukihiro Akeda, Takeshi Honda and Tetsuya Iida
  • Journal Title: PLoS ONE Volume: 5
  • DOI: 10.1371/journal.pone.0013365
  • Peer Reviewed
• [Journal Article] Transcription of Vibrio parahaemolyticus T3SS1 genes is regulated by a dual regulation system consisting of the ExsACDE regulatory cascade and H-NS (2010)
  • Author(s): Toshio Kodama, Chihiro Yamazaki, Kwon-Sam Park, Yukihiro Akeda, Tetsuya Iida and Takeshi Honda
  • Journal Title: FEMS Microbiology Letters Volume: 311 Pages: 10-17
  • DOI: 10.1111/j.1574-6968.2010.02066.x
  • Peer Reviewed
• [Journal Article] Impact of severity of pandemic influenza(H1N1) virus-associated community acquired pneumonia among adults in a tertiary hospital in Thailand (2010)
  • Author(s): Ratapum Champunot, Sansanee Tanjatham, Anusak Kerdsin, Parichart Puangpatra, Sunee Wangsai, Pornpit Treebuphachatsakul, Somboon Tasnsuphaswasdikul, Chavalit Kiatvitchukul, Harutaya Kasyanan, Jutharak Yimsabai, Yukihiro Akeda, Kazuyoshi Kawakami, Pathom Sawanpanyalert, Surang Dejsirilert, and Kazunori Oishi
  • Journal Title: Japanese Journal of Infectious Disease Volume: 63 Pages: 251-256
  • Peer Reviewed
• [Journal Article] Aeromonas hydrophila PepO outer membrane endopeptidase activates human big endothelin-3 in vitro and induces skin ulcer in goldfish(Carassius auratus) (2010)
  • Author(s): Said Kamal Abolghait, Yukihiro Akeda, Toshio Kodama, Vlademir V. Cantarelli, Tetsuya Iida and Takeshi Honda
  • Journal Title: Veterinary Microbiology Volume: 145 Pages: 113-121
  • DOI: 10.1016/j.vetmic.2010.03.009
  • Peer Reviewed
• [Journal Article] Two regulators of Vibrio parahaemolyticus play important roles in enterotoxicity by controlling the expression of genes in the Vp-PAI region (2010)
  • Author(s): Toshio Kodama, Kazuyoshi Gotoh, Hirotaka Hiyoshi, Mikiharu Morita, Kaori Izutsu, Yukihiro Akeda, Kwon-Sam Park, Vlademir Cantarelli, Rikard Dryselius, Tetsuya Iida and Takeshi Honda
  • Journal Title: PLoS ONE Volume: 5
  • DOI: 10.1371/journal.pone.0008678
  • Peer Reviewed
• [Journal Article] 高齢者感染症における重症化の予測因子2)微生物側の要因 (2010)
  • Author(s): 明田幸宏、大石和徳
  • Journal Title: 老年医学 Volume: 48 Pages: 1303-1306
• [Journal Article] Two regulators of Vibrio parahaemolyticus play important roles in enterotoxicity by controlling the expression of genes in the Vp-PAI region (2010)
  • Author(s): T Kodama, K Gotoh, H Hiyoshi, M Morita, K Izutsu, Y Akeda, KS Park, V Cantarelli, R Dryselius, T Iida, T Honda
  • Journal Title: PLoS One Volume: 5
  • Peer Reviewed
• [Journal Article] Aeromonas hydrophila PepO outer membrane endopeptidase activates human big endothelin-3 in vitro and induces skin ulcer in goldfish (Carassius auratus) (2010)
  • Author(s): SK Abolghait, Y Akeda, T Kodama, V Cantarelli, T Iida, T Honda
  • Journal Title: Veterinary Microbiology Volume: 145 Pages: 113-121
  • Peer Reviewed
• [Journal Article] Impact of severity of pandemic influenza (H1N1) virus-associated community-acquired pneumonia among adults in a tertiary hospital in Thailand. (2010)
  • Author(s): R Champunot, S Taniatham, A Kerdsin, P Puangpatra, S Wangsai, P Treebuphachatsakul, C Kiatvitchukul, H Kasyanan, J Yimsabai, Y Akeda, K Kawakami, P Sawanpanyalert, S Deisirilert, K Oishi
  • Journal Title: Japanese Journal of Infectious Diseases Volume: 63 Pages: 251-256
  • Peer Reviewed
• [Journal Article] Bile acid-induced virulence gene expression of Vbrio parahaemolyticus reveals a novel therapeutic potential for bile acid sequestrants (2010)
  • Author(s): K Goto, T Kodama, H Hiyoshi, K Izutsu, KS Park, R Dryselius, Y Akeda, T Honda, T Iida
  • Journal Title: PLoS One Volume: 5
  • Peer Reviewed
• [Journal Article] 高齢者感染症における重症化の予測因子(微生物側の要因) (2010)
  • Author(s): 明田幸宏、大石和徳
  • Journal Title: 老年医学 Volume: 48 Pages: 1303-1306
• [Presentation] マイクロアレイを用いたインフルエンザウイルス感染に伴う二次性細菌性肺炎発症に関与する宿主および細菌性因子の網羅的解析 (2012)
  • Author(s): 明田幸宏、江副浩和、大石和徳
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎県長崎市
• [Presentation] Identification of the Vibrio parahaemolyticus Type III Secretion System 2-Associated Chaperone VocC for the T3SS2-specific effector VopC (2011)
  • Author(s): Yukihiro Akeda, Toshio Kodama, Kazunobu Saito, Tetsuya Iida, Kazunori Oishi and Takeshi Honda
  • Organizer: Vibrio 2011
  • Place of Presentation: Santiago de Compostela, Spain, November
  • Year and Date: 2011-02-05
• [Presentation] 肝硬変患者にみられた新規血清型による豚レンサ球菌感染症の2例 (2011)
  • Author(s): 明田幸宏、大石和徳
  • Organizer: 第54回日本感染症学会中日本地方会学術集会
  • Place of Presentation: 奈良・奈良・奈良県新公会堂
• [Presentation] 北タイの肝硬変患者にみられた新規血清型による豚レンサ球菌感染症の2例 (2011)
  • Author(s): 明田幸宏、竹内壇、大石和徳
  • Organizer: 第52回日本熱帯医学会大会・第26回日本国際保健医療学会学術大会合同大会
  • Place of Presentation: 東京・東京大学本郷キャンパス
• [Presentation] 肝硬変患者にみられた新規血清型による豚レンサ球菌感染症の2例 (2011)
  • Author(s): 明田幸宏、大石和徳
  • Organizer: 第54回日本感染症学会中日本地方会学術集会
  • Place of Presentation: 奈良県奈良市
• [Presentation] 北タイの肝硬変患者にみられた新規血清型による豚レンサ球菌感染症の2例 (2011)
  • Author(s): 明田幸宏、竹内壇、大石和徳
  • Organizer: 第26回日本国際保健医療学会学術大会合同大会
  • Place of Presentation: 東京都文京区
• [Presentation] Identification of the Vibrio parahaemolyticus Type III Secretion System 2-Associated Chaperone VocC for the T3SS2-specific effector VopC (2011)
  • Author(s): Y Akeda, T Kodama, K Saito, T Iida, K Oishi, T Honda
  • Organizer: Vibrio 2011
  • Place of Presentation: Santiago de Compostela, Spain
• [Presentation] 大腸菌における腸炎ビブリオ3型分泌装置の構築 (2010)
  • Author(s): 明田幸宏、木村友美、山崎愛子、児玉年央、飯田哲也、本田武司
  • Organizer: 第44回腸炎ビブリオシンポジウム
  • Place of Presentation: 秋田県秋田ビューホテル
  • Year and Date: 2010-11-26
• [Presentation] 大腸菌における腸炎ビブリオ3型分泌装置の構築 (2010)
  • Author(s): 明田幸宏、木村友美、山崎愛子、児玉年央、飯田哲也、大石和徳、本田武司
  • Organizer: 第44回腸炎ビブリオシンポジウム
  • Place of Presentation: 秋田・秋田・秋田ビューホテル
• [Presentation] Impact of severity of pandemic influenza(H1N1) virus-associated community-acquired pneumonia among adults in a tertiary hospital in Thailand (2010)
  • Author(s): Ratapum Champunot, Sansanee Tanjatham, Anusak Kerdsin, Parichart Puangpatra, Sunee Wangsai, Pornpit Treebuphachatsakul, Somboon Tasnsuphaswasdikul, Chavalit Kiatvitchukul, Harutaya Kasyanan, Jutharak Yimsabai, Yukihiro Akeda, Kazuyoshi Kawakami, Pathom Sawanpanyalert, Surang Dejsirilert and Kazunori Oishi
  • Organizer: Asian-African Research Forum on Emerging and Reemerging Infections 2010
  • Place of Presentation: Hanoi, Vietnam
• [Book] 病原菌の今日的意味 (2011)
  • Author(s): 大石和徳、明田幸宏、田村和世(分担)
  • Total Pages: 799
  • Publisher: 医薬ジャーナル社
• [Book] 疾病と治療I 全身性/呼吸器系/循環器系/感染症/中毒/救急 (2010)
  • Author(s): 明田幸宏、大石和徳(共著)
  • Publisher: 南江堂