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Analysis of Vibrio parahaemolyticus T6SS apparatus and its ATPase

Research Project

Project/Area Number 22790406
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Bacteriology (including Mycology)
Research InstitutionOsaka University

Principal Investigator

AKEDA Yukihiro  大阪大学, 微生物病研究所, 特任講師 (60444527)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords腸炎ビブリオ / タンパク質分泌機構 / ATPase / 6型タンパク質分泌装置
Research Abstract

In this project, we analysed the structure of type 6 secretion system apparatus and its ATPase. Purification of T6SS associated ATPase was done and measured its activity, and also identified the proteins interacting with T6SS-specific secreted protein and its functional domains.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (36 results)

All 2012 2011 2010

All Journal Article (24 results) (of which Peer Reviewed: 21 results) Presentation (10 results) Book (2 results)

  • [Journal Article] Platelet Apoptosis and Apoptotic Platelet Clearance by Macrophages in Secondary Dengue Virus Infections.2012

    • Author(s)
      Alonzo, M., Lacuesta, T., Dimaano, EM., Kurosu, T., Suarez, LC., Mapua, CA Akeda, Y., Matias, RR., Kuter, DJ., Nagata, S., Natividad, FF. and Oishi, K.
    • Journal Title

      J Infect Dis.

      Volume: 205 Issue: 8 Pages: 1321-1329

    • DOI

      10.1093/infdis/jis180

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Population-based Study of Streptococcus suis Infection in Humans in Phayao Province in Northern Thailand2012

    • Author(s)
      Takeuchi D, Kerdsin A, Pienpringam A, Loetthong P, Samerchea S, Pakkinee Loetthong P, Khamisra K, Wongwan N, Areeratana P, Chiranairadul P, Lertchayanti S, Petcharat S, Yowang A, Chaiwongsaen P, Nakayama T, Akeda Y, Hamada S, Sawanpanyalert P, Dejsirilert
    • Journal Title

      PLoS ONE

      Volume: 7(2) Issue: 2 Pages: e31265-e31265

    • DOI

      10.1371/journal.pone.0031265

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 肺炎球菌ワクチン2012

    • Author(s)
      明田幸宏、大石和徳
    • Journal Title

      診断と治療

      Volume: 100 Pages: 455-458

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Journal Article] Recombinant AexU effector protein of Aeromonas veronii bv. sobria disrupts the actin cytoskeleton by downregulation of Rac1 and induces direct cytotoxicity to・4-integrin expressing cell lines2011

    • Author(s)
      Said Kamal Abolghait, Tetsuya Iida, Toshio Kodama, Vlademir V. Cantarelli, Yukihiro Akeda and Takeshi Honda
    • Journal Title

      Microbial Pathogenesis

      Volume: 51 Issue: 6 Pages: 454-465

    • DOI

      10.1016/j.micpath.2011.09.006

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] VopV, an F-Actin-Binding Type III Secretion Effector, Is Required for Vibrio parahaemolyticus-Induced Enterotoxicity2011

    • Author(s)
      Hirotaka Hiyoshi, Toshio Kodama, Kazunobu Saito, Kazuyoshi Gotoh, Shigeaki Matsuda, Yukihiro Akeda, Takeshi Honda and Tetsuya Iida
    • Journal Title

      Cell Host & Microbe

      Volume: 10 Issue: 4 Pages: 401-409

    • DOI

      10.1016/j.chom.2011.08.014

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of the Vibrio parahaemolyticus Type III Secretion System 2-Associated Chaperone VocC for the T3SS2-Specific Effector VopC2011

    • Author(s)
      Yukihiro Akeda, Toshio Kodama, Kazunobu Saito, Tetsuya Iida, Kazunori Oishi and Takeshi Honda
    • Journal Title

      FEMS Microbiology Letters

      Volume: 324 Issue: 2 Pages: 156-164

    • DOI

      10.1111/j.1574-6968.2011.02399.x

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sepsis and spontaneous bacterial peritonitis in Thailand2011

    • Author(s)
      Kerdsin A, Dejsirilert S, Sawanpanyalert P, Boonnark A, Noithachang W, Sriyakun D, Simkum S, Chokngam S, Gottschalk M, Akeda Y, Oishi K
    • Journal Title

      Lancet

      Volume: 378 Issue: 9794 Pages: 960-960

    • DOI

      10.1016/s0140-6736(11)60923-9

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genotypic profile of Streptococcus suis serotype 2 and clinical features of infection in humans, Thailand2011

    • Author(s)
      Kerdsin A, Dejsirilert S, Puangpatra P, Sripakdee S, Chumla K, Boonkerd N, Polwichai P, Tanimura S, Takeuchi D, Nakayama T, Nakamura S, Akeda Y, Gottschalk M, Sawanpanyalert P, Oishi K
    • Journal Title

      Emerg Infect Dis

      Volume: 17 Issue: 5 Pages: 835-842

    • DOI

      10.3201/eid1705.100754

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Comparative Effects of Toll-Like Receptor Agonists on a Low Dose PspA Intranasal Vaccine against Fatal Pneumococcal Pneumonia in Mice2011

    • Author(s)
      Zhenyu Piao, Keita Oma, Hirokazu Ezoe, Yukihiro Akeda, Kazunori Tomono and Kazunori Oishi
    • Journal Title

      Journal of Vaccines and Vaccination

      Volume: 2 Issue: 01 Pages: 113-113

    • DOI

      10.4172/2157-7560.1000113

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Intranasal vaccination with pneumococcal surface protein A plus poly(I : C) protects against secondary pneumococcal pneumonia in mice2011

    • Author(s)
      Hirokazu Ezoe, Yukihiro Akeda, Taiki Aoshi, , Zenyo Piao, Shohei Koyama, Takeshi Tanimoto, Ken J Ishii and Kazunori Oishi
    • Journal Title

      Vaccine

      Volume: 29 Issue: 9 Pages: 1754-1761

    • DOI

      10.1016/j.vaccine.2010.12.117

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Streptococcus suis infection induces to bacterial accumulation in the kidney2011

    • Author(s)
      Tatsuya Nakayama, Dan Takeuchi, Yukihiro Akeda, Kazunori Oishi
    • Journal Title

      Microbial Pathogenesis

      Volume: 50 Issue: 2 Pages: 87-93

    • DOI

      10.1016/j.micpath.2010.11.005

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intranasal vaccination with pneumococcal surface protein A plus poly(I:C) protects against secondary pneumococcal pneumonia in mice2011

    • Author(s)
      H Ezoe, Y Akeda, T Aoshi, Z Piao, S Koyama, T Tanimoto, KJ Ishii, K Oishi
    • Journal Title

      Vaccine

      Volume: 29 Pages: 1754-1761

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Clinical features of Streptococcus suis serotype 2 infections in relation to the genotypic profiles of isolates in Thailand.2011

    • Author(s)
      A Kerdsin, S Deisirilert, P Puangpatra, S Sripakdee, K Chumla, N Boonkerd, P Polwichai, S Tanimura, D Takeuchi, T Nakayama, S Nakamura, Y Akeda, P Sawanpanyalert, K Oishi
    • Journal Title

      Emerging Infectious Diseases

      Volume: (印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bile acid-induced virulence gene expression of Vibrio parahaemolyticus reveals a novel therapeutic potential for bile acid sequestrants2010

    • Author(s)
      Kazuyoshi Goto, Toshio Kodama, Hirotaka Hiyoshi, Kaori Izutsu, Kwon-Sam Park, Rikard Dryselius, Yukihiro Akeda, Takeshi Honda and Tetsuya Iida
    • Journal Title

      PLoS ONE

      Volume: 5 Issue: 10 Pages: e13365-e13365

    • DOI

      10.1371/journal.pone.0013365

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transcription of Vibrio parahaemolyticus T3SS1 genes is regulated by a dual regulation system consisting of the ExsACDE regulatory cascade and H-NS2010

    • Author(s)
      Toshio Kodama, Chihiro Yamazaki, Kwon-Sam Park, Yukihiro Akeda, Tetsuya Iida and Takeshi Honda
    • Journal Title

      FEMS Microbiology Letters

      Volume: 311 Issue: 1 Pages: 10-17

    • DOI

      10.1111/j.1574-6968.2010.02066.x

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Impact of severity of pandemic influenza(H1N1) virus-associated community acquired pneumonia among adults in a tertiary hospital in Thailand2010

    • Author(s)
      Ratapum Champunot, Sansanee Tanjatham, Anusak Kerdsin, Parichart Puangpatra, Sunee Wangsai, Pornpit Treebuphachatsakul, Somboon Tasnsuphaswasdikul, Chavalit Kiatvitchukul, Harutaya Kasyanan, Jutharak Yimsabai, Yukihiro Akeda, Kazuyoshi Kawakami, Pathom Sawanpanyalert, Surang Dejsirilert, and Kazunori Oishi
    • Journal Title

      Japanese Journal of Infectious Disease

      Volume: 63 Pages: 251-256

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Aeromonas hydrophila PepO outer membrane endopeptidase activates human big endothelin-3 in vitro and induces skin ulcer in goldfish(Carassius auratus)2010

    • Author(s)
      Said Kamal Abolghait, Yukihiro Akeda, Toshio Kodama, Vlademir V. Cantarelli, Tetsuya Iida and Takeshi Honda
    • Journal Title

      Veterinary Microbiology

      Volume: 145 Issue: 1-2 Pages: 113-121

    • DOI

      10.1016/j.vetmic.2010.03.009

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Two regulators of Vibrio parahaemolyticus play important roles in enterotoxicity by controlling the expression of genes in the Vp-PAI region2010

    • Author(s)
      Toshio Kodama, Kazuyoshi Gotoh, Hirotaka Hiyoshi, Mikiharu Morita, Kaori Izutsu, Yukihiro Akeda, Kwon-Sam Park, Vlademir Cantarelli, Rikard Dryselius, Tetsuya Iida and Takeshi Honda
    • Journal Title

      PLoS ONE

      Volume: 5 Issue: 1 Pages: e8678-e8678

    • DOI

      10.1371/journal.pone.0008678

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 高齢者感染症における重症化の予測因子2)微生物側の要因2010

    • Author(s)
      明田幸宏、大石和徳
    • Journal Title

      老年医学

      Volume: 48 Pages: 1303-1306

    • Related Report
      2011 Final Research Report
  • [Journal Article] Two regulators of Vibrio parahaemolyticus play important roles in enterotoxicity by controlling the expression of genes in the Vp-PAI region2010

    • Author(s)
      T Kodama, K Gotoh, H Hiyoshi, M Morita, K Izutsu, Y Akeda, KS Park, V Cantarelli, R Dryselius, T Iida, T Honda
    • Journal Title

      PLoS One

      Volume: 5

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Aeromonas hydrophila PepO outer membrane endopeptidase activates human big endothelin-3 in vitro and induces skin ulcer in goldfish (Carassius auratus)2010

    • Author(s)
      SK Abolghait, Y Akeda, T Kodama, V Cantarelli, T Iida, T Honda
    • Journal Title

      Veterinary Microbiology

      Volume: 145 Pages: 113-121

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Impact of severity of pandemic influenza (H1N1) virus-associated community-acquired pneumonia among adults in a tertiary hospital in Thailand.2010

    • Author(s)
      R Champunot, S Taniatham, A Kerdsin, P Puangpatra, S Wangsai, P Treebuphachatsakul, C Kiatvitchukul, H Kasyanan, J Yimsabai, Y Akeda, K Kawakami, P Sawanpanyalert, S Deisirilert, K Oishi
    • Journal Title

      Japanese Journal of Infectious Diseases

      Volume: 63 Pages: 251-256

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bile acid-induced virulence gene expression of Vbrio parahaemolyticus reveals a novel therapeutic potential for bile acid sequestrants2010

    • Author(s)
      K Goto, T Kodama, H Hiyoshi, K Izutsu, KS Park, R Dryselius, Y Akeda, T Honda, T Iida
    • Journal Title

      PLoS One

      Volume: 5

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 高齢者感染症における重症化の予測因子(微生物側の要因)2010

    • Author(s)
      明田幸宏、大石和徳
    • Journal Title

      老年医学

      Volume: 48 Pages: 1303-1306

    • Related Report
      2010 Annual Research Report
  • [Presentation] マイクロアレイを用いたインフルエンザウイルス感染に伴う二次性細菌性肺炎発症に関与する宿主および細菌性因子の網羅的解析2012

    • Author(s)
      明田幸宏、江副浩和、大石和徳
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎県長崎市
    • Related Report
      2011 Annual Research Report
  • [Presentation] Identification of the Vibrio parahaemolyticus Type III Secretion System 2-Associated Chaperone VocC for the T3SS2-specific effector VopC2011

    • Author(s)
      Yukihiro Akeda, Toshio Kodama, Kazunobu Saito, Tetsuya Iida, Kazunori Oishi and Takeshi Honda
    • Organizer
      Vibrio 2011
    • Place of Presentation
      Santiago de Compostela, Spain, November
    • Year and Date
      2011-02-05
    • Related Report
      2011 Final Research Report
  • [Presentation] 肝硬変患者にみられた新規血清型による豚レンサ球菌感染症の2例2011

    • Author(s)
      明田幸宏、大石和徳
    • Organizer
      第54回日本感染症学会中日本地方会学術集会
    • Place of Presentation
      奈良・奈良・奈良県新公会堂
    • Related Report
      2011 Final Research Report
  • [Presentation] 北タイの肝硬変患者にみられた新規血清型による豚レンサ球菌感染症の2例2011

    • Author(s)
      明田幸宏、竹内壇、大石和徳
    • Organizer
      第52回日本熱帯医学会大会・第26回日本国際保健医療学会学術大会合同大会
    • Place of Presentation
      東京・東京大学本郷キャンパス
    • Related Report
      2011 Final Research Report
  • [Presentation] 肝硬変患者にみられた新規血清型による豚レンサ球菌感染症の2例2011

    • Author(s)
      明田幸宏、大石和徳
    • Organizer
      第54回日本感染症学会中日本地方会学術集会
    • Place of Presentation
      奈良県奈良市
    • Related Report
      2011 Annual Research Report
  • [Presentation] 北タイの肝硬変患者にみられた新規血清型による豚レンサ球菌感染症の2例2011

    • Author(s)
      明田幸宏、竹内壇、大石和徳
    • Organizer
      第26回日本国際保健医療学会学術大会合同大会
    • Place of Presentation
      東京都文京区
    • Related Report
      2011 Annual Research Report
  • [Presentation] Identification of the Vibrio parahaemolyticus Type III Secretion System 2-Associated Chaperone VocC for the T3SS2-specific effector VopC2011

    • Author(s)
      Y Akeda, T Kodama, K Saito, T Iida, K Oishi, T Honda
    • Organizer
      Vibrio 2011
    • Place of Presentation
      Santiago de Compostela, Spain
    • Related Report
      2011 Annual Research Report
  • [Presentation] 大腸菌における腸炎ビブリオ3型分泌装置の構築2010

    • Author(s)
      明田幸宏、木村友美、山崎愛子、児玉年央、飯田哲也、本田武司
    • Organizer
      第44回腸炎ビブリオシンポジウム
    • Place of Presentation
      秋田県秋田ビューホテル
    • Year and Date
      2010-11-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] 大腸菌における腸炎ビブリオ3型分泌装置の構築2010

    • Author(s)
      明田幸宏、木村友美、山崎愛子、児玉年央、飯田哲也、大石和徳、本田武司
    • Organizer
      第44回腸炎ビブリオシンポジウム
    • Place of Presentation
      秋田・秋田・秋田ビューホテル
    • Related Report
      2011 Final Research Report
  • [Presentation] Impact of severity of pandemic influenza(H1N1) virus-associated community-acquired pneumonia among adults in a tertiary hospital in Thailand2010

    • Author(s)
      Ratapum Champunot, Sansanee Tanjatham, Anusak Kerdsin, Parichart Puangpatra, Sunee Wangsai, Pornpit Treebuphachatsakul, Somboon Tasnsuphaswasdikul, Chavalit Kiatvitchukul, Harutaya Kasyanan, Jutharak Yimsabai, Yukihiro Akeda, Kazuyoshi Kawakami, Pathom Sawanpanyalert, Surang Dejsirilert and Kazunori Oishi
    • Organizer
      Asian-African Research Forum on Emerging and Reemerging Infections 2010
    • Place of Presentation
      Hanoi, Vietnam
    • Related Report
      2011 Final Research Report
  • [Book] 病原菌の今日的意味2011

    • Author(s)
      大石和徳、明田幸宏、田村和世(分担)
    • Total Pages
      799
    • Publisher
      医薬ジャーナル社
    • Related Report
      2011 Annual Research Report
  • [Book] 疾病と治療I 全身性/呼吸器系/循環器系/感染症/中毒/救急2010

    • Author(s)
      明田幸宏、大石和徳(共著)
    • Publisher
      南江堂
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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