| Project/Area Number |
22790409
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | The University of Tokushima |
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Principal Investigator |
NAKAYAMA Haruyuki 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教 (80294669)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | Bacteroides fragilis / 莢膜 / 相変異 / 腸管共生 / DNA逆位 / 腸管免疫 / 腸管常在菌 / T細胞分化 / 共生 |
|---|
| Research Abstract |
Bacteroides fragilis strain YCH46 possesses master DNA invertases that regulate DNA inversions at multiple loci simultaneously. One is Mpi that mediates the on-off switching of seven promoter regions for capsular polysaccharide(PS) biosynthesis. Dispite the importance of PS synthesis for intestinal colonization, little is known about PS regulation in vitro and in vivo. While this organism may theoretically display up to 128 distinct polysaccharide combinations, the PS3 promoter was only on in greater than 12% of bacteria grown in vitro. Intestinal lamina propria lymphocytes from PS3-deleted B. fragilis mono-associated mice displayed decreased percentages of CD4^+ IL-4^+ T cells compared with control mice.
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