Development of a mouse model of the infection with Streptococcus dysgalactiae subspecies equisimilis.

• Project/Area Number: 22790412
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: Kitasato University
• Principal Investigator: YOSHIDA Haruno 北里大学, その他の研究科, 助手 (70563386)
• Co-Investigator(Renkei-kenkyūsha): TAKAHASHI Takashi 北里大学, 感染制御科学府, 教授 (00292855) ; MATSUI Hidenori 北里大学, 感染制御科学府, 講師 (30219373) ; MURAYAMA Somay (YAMAGATA Somay) 日本大学, 薬学部薬学科, 准教授 (60183654)
• Project Period (FY): 2010-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,899,459 (Direct Cost: ¥3,768,815、Indirect Cost: ¥1,130,644); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,129,459 (Direct Cost: ¥868,815、Indirect Cost: ¥260,644); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: レンサ球菌 / 劇症型感染症 / SDSE / 感染モデル動物 / G群レンサ球菌 / 劇症型 / マウスモデル / ゲノム解析 / A群レンサ球菌 / CD46 / トランスジェニック

Research Abstract

Ankle arthritis was induced by a single subcutaneous infection od the Streptococcus dysgalactiae subspecies equisimilis strain RE378, which was isolated from a patient suffering from multiple organ failure due to septicaemia, into both hind footpads of human CD46-expressing transgenic(Tg) mice. The histopathological findings in the hind ankle sections of CD46 Tg mice showed the stimulation of osteoclast formation associated with inflammation of the synovial membrane. The comparative genome sequence analysis revealed that the 43 predicted virulence-related factors including putative adhesion proteins and nucleases.

Research Products

• [Journal Article] 溶血性連鎖球菌と化膿性関節炎 (2014)
  • Author(s): 高橋 孝, 吉田春乃, 松井英則
  • Journal Title: Medical Practice Volume: 31(1) Pages: 147-147
• [Journal Article] Interleukin- 1β Response of Peritoneal Macrophages to Streptococcus pyogenes Exposure : Differential Response to Living and Heat- killed Bacteria (2013)
  • Author(s): Tokiyo Matoba, Yuriko Nakatani, Kazuaki Arai, Hidenori Matsui, Haruno Yoshida, Takashi Takahashi
  • Journal Title: Journal of Experimental &Clinical Medicine Volume: 5巻 Issue: 6 Pages: 227-230
  • DOI: 10.1016/j.jecm.2013.10.011
  • Peer Reviewed
• [Journal Article] レンサ球菌性局所壊死における肥満細胞の役割 (2012)
  • Author(s): 松井英則, 吉田春乃, 高橋 孝
  • Journal Title: Medical Practice Volume: 29(2) Pages: 340-340
• [Journal Article] A CD46 transgenic mouse model for studying the histopathology of arthritis caused by subcutaneous infection with Streptococcus dysgalactiae subspecies equisimilis (2012)
  • Author(s): Yoshida, H., Matsui, H.Murayama, S.Y., Takada, Y., Matsuo, K.Takahashi, T., Nakamura, M., Ubukata, K., Takahashi, T.
  • Journal Title: J.Med.Microb. Volume: 60 Issue: 12 Pages: 1860-1868
  • DOI: 10.1099/jmm.0.034108-0
  • Peer Reviewed
• [Journal Article] Darmal mast cells reduce progressive tissue necrosis caused by subcutaneous infection with Streptococcus pyogenes in mice (2011)
  • Author(s): Hidenori Matsui, Yukie Sekiya, Tetsufumi Takahashi, Masahiko Nakamura, Ken'ichi Imanishi, Haruno Yoshida, Somay Yamagata Murayama, Takashi Takahashi, Kanji Tsuchimoto, Takehiko Uchiyama, and Kimiko Ubukata
  • Journal Title: Journal of Medical Microbiology Volume: 60巻 Issue: 1 Pages: 128-134
  • DOI: 10.1099/jmm.0.020495-0
  • Peer Reviewed
• [Journal Article] Darmal mast cells reduce progressive tissue necrosis caused by subcutaneous infection with Streptococcus pyogenes in mice. (2011)
  • Author(s): Hidenori Matsui, Yukie Sekiya, Tetsufumi Takahashi, Masahiko Nakamura, Ken'ichi Imanishi, Haruno Yoshida, Somay Yamagata Murayama, Takashi Takahashi, Kanji Tsuchimoto, Takehiko Uchiyama, Kimiko Ubukata.
  • Journal Title: Journal of Medical Microbiology Volume: 60 Pages: 128-134
  • Peer Reviewed
• [Journal Article] Candidatus Helicobacter heilmannii" infection in the course of gastric mucosa-associated lymphoid tissue (MALT) lymphoma in human beings and mouse models. (2010)
  • Author(s): Hidenori Matsui, Yukie Sekiya, Chihiro Aikawa, Soamy Yamagata Murayama, Tetsufumi Takahashi, Haruno Yoshida, Takashi Takahashi, Kanji Tsuchimoto, Masahiko Nakamura.
  • Journal Title: Current Trends in Microbilogy Volume: 6 Pages: 1-8
  • Peer Reviewed
• [Journal Article] ナノサイズ免疫磁気ビーズと従来の免疫磁気ビーズを用いた食品検体からの腸管出血性大腸菌の分離の比較について (2010)
  • Author(s): 吉田春乃、松井英則
  • Journal Title: 医療と検査機器・試薬 Volume: 33 Pages: 241-245
  • Peer Reviewed
• [Presentation] Interleukin-1b response of peritoneal macrophages to Streptococcus pyogenes exposure : differential response to living and heat-killed bacteria (2014)
  • Author(s): Takashi Takahashi, Haruno Yoshida, Kazuaki Arai, and Hidenori Matsui
  • Organizer: Beijing International Conference of Infection Control, People's Republic of China
  • Place of Presentation: 北京(中国)
  • Year and Date: 2014-03-21
• [Presentation] 髄膜炎由来レンサ球菌の強毒化に関わる分子メカニズムの解析 (2013)
  • Author(s): 吉田春乃
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸国際展示場(兵庫県)
  • Year and Date: 2013-12-05
• [Presentation] rocAの1遺伝子変異による化膿レンサ球菌の高病原性化 (2013)
  • Author(s): 吉田春乃
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 幕張メッセ(千葉県)
  • Year and Date: 2013-03-18
• [Presentation] rocAの1遺伝子変異による化膿レンサ球菌の高病原性化 (2013)
  • Author(s): 吉田春乃
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 幕張メッセ（千葉県）
• [Presentation] CD46 transgenic mouse model of acute severe invasive infections caused by Streptococcus pyogenes (2011)
  • Author(s): Hidenori Matsui, Haruno Yoshida, Somay Y Murayama, Asako Takizawa, Osamu Takeuchi, Tetsufumi Takahashi, Masahiko Nakamura, Ken'Ichi Imanishi, Takehiko Uchiyama, Takashi Takahashi, and Kimiko Ubukata
  • Organizer: International Union of Microbiological Societies 2011
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2011-07-09
• [Presentation] 劇症型A群溶血性レンサ球菌感染症による骨壊死の解析 (2011)
  • Author(s): 吉田春乃
  • Organizer: 第85回日本感染症学会総会学術講演会
  • Place of Presentation: ザ・プリンス パークタワー(東京都)
  • Year and Date: 2011-04-22
• [Presentation] 劇症型A群溶血性レンサ球菌感染症による骨壊死の解析 (2011)
  • Author(s): 吉田春乃、松井英則、村山そう明、生方公子、高橋孝
  • Organizer: 第85回日本感染症学会総会学術講演会
  • Place of Presentation: 東京都港区ザ・プリンス パークタワー
  • Year and Date: 2011-04-22
• [Presentation] CD46 トランスジェニックマウスを用いたStreptococcus dysgalactiae subsp. Equisimilis (SDSE)感染モデルの構築 (2010)
  • Author(s): 松井英則, 吉田春乃
  • Organizer: 第84回日本感染症学会総会
  • Place of Presentation: 京都大学医学部芝欄会館(京都府)
  • Year and Date: 2010-04-05
• [Presentation] CD46トランスジェニックマウスを用いたStreptococcus dysgalactiae subsp.equisimilis(SDSE)感染モデルの構築 (2010)
  • Author(s): 松井英則、吉田春乃、岡田圭祐、村山〓明、生方公子
  • Organizer: 第84回日本感染症学会総会
  • Place of Presentation: 京都大学医学部芝欄会館
  • Year and Date: 2010-04-05
• [Presentation] Comparative genome analysis of Streptococcus pyogenes MTB313 and MTB314, isolated from a patient suffering from acute streptococcal meningitis
  • Author(s): Haruno Yoshida, Hidenori Matsui, Takashi Takahashi
  • Organizer: 第36回 日本分子生物学会
  • Place of Presentation: 神戸国際展示場（兵庫県神戸市）
• [Remarks] 北里大学感染制御科学府 感染症学研究室
  • URL: http://www.kitasato-u.ac.jp/lisci/life/chart/LSI-lab15.html
• [Remarks] 北里大学大学院感染制御科学府感染症学研究室紹介HP
  • URL: http://www.kitasato-u.ac.jp/research/gakubu/k111101107.html