Comprehensive identification of host factors involving in ebolavirus replication
Project/Area Number |
22790425
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Virology
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Research Institution | The University of Tokyo |
Principal Investigator |
OZAWA Makoto 東京大学, 医科学研究所, 特任助教 (50568722)
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Project Period (FY) |
2010 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | ウイルス / 細胞 / エボラウイルス / Mutagenesis / 宿主因子 / 網羅的同定 / Mutagnesis |
Research Abstract |
For comprehensive identification of host factors involving in replication of ebolavirus, we sought to establish a screening system for such fact factors by using genetically-engineered biologically contained ebolavirus. A human-derived cell line KBM-7, which had been thought to be the most suitable for host factor screening/identification was turned out to be resistant to ebolavirus infection, suggesting that this cell line dose not express an unknown host factor(s) required for ebolavirus infection. By contrast, a hamster-derived cell line CHO was susceptible to ebolavirus, indicating that this screening project can be carried out based on CHO cells.
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Report
(3 results)
Research Products
(17 results)
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[Journal Article] Avian-type receptor-binding ability can increase influenza virus pathogenicity in macaques2011
Author(s)
Watanabe T, Shinya K, Watanabe S, Imai M, Hatta M, Li C, Wolter BE Neumann G, Hanson A, Ozawa M, Yamada S, Imai H, Sakabe S, Takano R, Iwatsuki-Horimoto K, Kiso M, Ito M, Fukuyama S, Kawakami E, Gorai T, et al
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Journal Title
Journal of Virology
Volume: 85(24)
Issue: 24
Pages: 13195-13203
DOI
Related Report
Peer Reviewed
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[Journal Article] Replication-incompetent influenza A viruses that stably express a foreign gene2011
Author(s)
Ozawa M, Victor ST, Taft AS, Yamada S, Li C, Hatta M, Das SC, Takashita E, Kakugawa S, Maher EA, Neumann G, Kawaoka Y
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Journal Title
J Gen Virol
Volume: 92
Issue: 12
Pages: 2879-2888
DOI
Related Report
Peer Reviewed
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[Presentation] Replication-incompetent influenza A viruses that stably express a foreign gene2011
Author(s)
Ozawa M, Victor ST, Taft AS, Yamada S, Li C, Hatta M, Das SC, Takashita E, Kakugawa S, Maher EA, Neumann G, Kawaoka Y
Organizer
IUMS (International Union of Microbiological Societies) 2011 Congress : International Congress of Virology
Place of Presentation
札幌(日本)
Year and Date
2011-09-13
Related Report
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[Presentation] Identification of an influenza A viral RNA transcription/replication inhibitor by using viral ribonucleoprotein complex-expressing cells2011
Author(s)
Ozawa M, Shimojima M, Goto H, Watanabe S, Hatta Y, Kiso M, Furuta Y, Horimoto T, Peters NR, Hoffmann FM, Kawaoka Y
Organizer
Keystone Symposia, Pathogenesis of Influenza : Virus-Host Interactions
Place of Presentation
香港(中国)
Year and Date
2011-05-26
Related Report
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