Comprehensive identification of host factors involving in ebolavirus replication

• Project/Area Number: 22790425
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: The University of Tokyo
• Principal Investigator: OZAWA Makoto 東京大学, 医科学研究所, 特任助教 (50568722)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ウイルス / 細胞 / エボラウイルス / Mutagenesis / 宿主因子 / 網羅的同定 / Mutagnesis

Research Abstract

For comprehensive identification of host factors involving in replication of ebolavirus, we sought to establish a screening system for such fact factors by using genetically-engineered biologically contained ebolavirus. A human-derived cell line KBM-7, which had been thought to be the most suitable for host factor screening/identification was turned out to be resistant to ebolavirus infection, suggesting that this cell line dose not express an unknown host factor(s) required for ebolavirus infection. By contrast, a hamster-derived cell line CHO was susceptible to ebolavirus, indicating that this screening project can be carried out based on CHO cells.

Research Products

• [Journal Article] A Replication-Incompetent PB2-Knockout Influenza A Virus Vaccine Vector (2012)
  • Author(s): Victor ST, Watanabe S, Katsura H, Ozawa M, Kawaoka Y
  • Journal Title: J Virol Volume: 86 Issue: 8 Pages: 4123-4128
  • DOI: 10.1128/jvi.06232-11
  • Peer Reviewed
• [Journal Article] The highly conserved arginine residues at positions 76 through 78 of influenza A virus matrix protein M1 play an important role in viral replication by affecting the intracellular localization of M1 (2012)
  • Author(s): Das SC, Watanabe S, Hatta M, Noda T, Neumann G, Ozawa M, Kawaoka Y
  • Journal Title: J Virol Volume: 86 Issue: 3 Pages: 1522-1530
  • DOI: 10.1128/jvi.06230-11
  • Peer Reviewed
• [Journal Article] Taming influenza viruses (2011)
  • Author(s): Ozawa M, Kawaoka Y
  • Journal Title: Virus Res Volume: 162 Issue: 1-2 Pages: 8-11
  • DOI: 10.1016/j.virusres.2011.09.035
  • Peer Reviewed
• [Journal Article] Avian-type receptor-binding ability can increase influenza virus pathogenicity in macaques (2011)
  • Author(s): Watanabe T, Shinya K, Watanabe S, Imai M, Hatta M, Li C, Wolter BE Neumann G, Hanson A, Ozawa M, Yamada S, Imai H, Sakabe S, Takano R, Iwatsuki-Horimoto K, Kiso M, Ito M, Fukuyama S, Kawakami E, Gorai T, et al
  • Journal Title: Journal of Virology Volume: 85(24) Issue: 24 Pages: 13195-13203
  • DOI: 10.1128/jvi.00859-11
  • Peer Reviewed
• [Journal Article] Replication-incompetent influenza A viruses that stably express a foreign gene (2011)
  • Author(s): Ozawa M, Victor ST, Taft AS, Yamada S, Li C, Hatta M, Das SC, Takashita E, Kakugawa S, Maher EA, Neumann G, Kawaoka Y
  • Journal Title: J Gen Virol Volume: 92 Issue: 12 Pages: 2879-2888
  • DOI: 10.1099/vir.0.037648-0
  • Peer Reviewed
• [Journal Article] Mutations in PA, NP, and HA of a pandemic (H1N1) 2009 influenza virus contribute to its adaptation to mice (2011)
  • Author(s): Sakabe S, Ozawa M, Takano R, Iwatsuki-Horimoto K, Kawaoka Y
  • Journal Title: Virus Research Volume: 158(1-2) Issue: 1-2 Pages: 124-129
  • DOI: 10.1016/j.virusres.2011.03.022
  • Peer Reviewed
• [Journal Article] Subclinical Brain Injury Caused by H5N1 Influenza Virus Infection (2011)
  • Author(s): Shinya K, Makino A, Hatta M, Watanabe S, Kim JH, Hatta Y, Gao P, Ozawa M, Le QM, Kawaoka Y
  • Journal Title: J Virol Volume: 85 Issue: 10 Pages: 5202-5207
  • DOI: 10.1128/jvi.00239-11
  • Peer Reviewed
• [Journal Article] Effect of an asparagine-to-serine mutation at position 294 in neuraminidase on the pathogenicity of highly pathogenic H5N1 influenza A virus (2011)
  • Author(s): Kiso M, Ozawa M, Le MQ, Imai H, Takahashi K, Kakugawa S, Noda T, Horimoto T, Kawaoka Y
  • Journal Title: J Virol Volume: 85 Issue: 10 Pages: 860-866
  • DOI: 10.1128/jvi.00047-11
  • Peer Reviewed
• [Journal Article] Impact of Amino Acid Mutations in PB2, PB1-F2, and NS1 on the Replication and Pathogenicity of Pandemic(H1N1) 2009 Influenza Viruses. (2009)
  • Author(s): Ozawa M, Basnet S, Burley LM, Neumann G, Hatta M, Kawaoka Y.
  • Journal Title: J Virol Volume: 85 Issue: 9 Pages: 4596-4601
  • DOI: 10.1128/jvi.00029-11
  • Peer Reviewed
• [Presentation] Replication-incompetent influenza A viruses that stably express a foreign gene (2011)
  • Author(s): Ozawa M, Victor ST, Taft AS, Yamada S, Li C, Hatta M, Das SC, Takashita E, Kakugawa S, Maher EA, Neumann G, Kawaoka Y
  • Organizer: IUMS (International Union of Microbiological Societies) 2011 Congress : International Congress of Virology
  • Place of Presentation: 札幌(日本)
  • Year and Date: 2011-09-13
• [Presentation] Identification of an influenza A viral RNA transcription/replication inhibitor by using viral ribonucleoprotein complex-expressing cells (2011)
  • Author(s): Ozawa M, Shimojima M, Goto H, Watanabe S, Hatta Y, Kiso M, Furuta Y, Horimoto T, Peters NR, Hoffmann FM, Kawaoka Y
  • Organizer: Keystone Symposia, Pathogenesis of Influenza : Virus-Host Interactions
  • Place of Presentation: 香港(中国)
  • Year and Date: 2011-05-26
• [Presentation] Impact of amino acid mutations in PB2, PB1-F2, and NS1 on the replication and pathogenicity of pandemic (H1N1) 2009 influenza viruses (2011)
  • Author(s): Ozawa M, Basnet S, Burley LM, Neumann G, Hatta M, Kawaoka Y
  • Organizer: 5th Annual CEIRS (Centers of Excellence for Influenza Research and Surveillance) Meeting
  • Place of Presentation: アトランタ(アメリカ)
  • Year and Date: 2011-04-19
• [Presentation] Replication-incompetent influenza A viruses that stably express a foreign gene (2011)
  • Author(s): Ozawa M et al
  • Organizer: XV International Congress of Virology
  • Place of Presentation: Sapporo, Japan
• [Presentation] Identification of an influenza A viral RNA transcription/replication inhibitor by using viral ribonucleoprotein complex-expressing cells (2011)
  • Author(s): Ozawa M et al
  • Organizer: KeystoneSymposia, Pathogenesis of Influenza : Virus-Host Interactions
  • Place of Presentation: Hong Kong SAR, China
• [Presentation] Impact of amino acid mutations in PB2, PB1-F2, and NS1 on the replication and pathogenicity of pandemic(H1N1) 2009 influenza viruses (2011)
  • Author(s): Ozawa M et al
  • Organizer: 5^<12> Annual CEIRS(Centers of Excellence for Influenza Research and Surveillance) Meeting
  • Place of Presentation: Atlanta, USA
• [Patent(Industrial Property Rights)] Novel influenza virus vaccines (2011)
  • Inventor(s): Kawaoka Y, Neumann G, Ozawa M
  • Industrial Property Rights Holder: University of Wisconsin-Madison
  • Filing Date: 2011-08-26
  • Overseas
• [Patent(Industrial Property Rights)] Novel influenza virus vaccines (2011)
  • Inventor(s): Kawaoka Y, Neumann G, Ozawa M
  • Industrial Property Rights Holder: University of Wisconsin-Madison
  • Filing Date: 2011-08-26
  • Overseas