Analysis of mechanism of latent infection and reactivation of varicella-zoster virus

• Project/Area Number: 22790428
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: University of Toyama
• Principal Investigator: TAKEMOTO Masaya 富山大学, 大学院・医学薬学研究部(医学), 助教 (60379237)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 抗原変調AV3(antigenic modulation) / shedding microvesicles / オートファジー / 水痘帯状疱疹ウイルス / glycoprotein H / glycoprotein H (gH) / 小胞体ストレス

Research Abstract

Monoclonal antibody against glycoprotein H(gH) encoded by varicella-zoster virus(VZV) terminates lytic replication of VZV and induces the quiescently infected cells with dormant but recoverable VZV infectivity. It was demonstrated that autophagic clearance of intracellular gH and shedding microvesicle-mediated VZV virion outside the infected cells.

Research Products

• [Journal Article] Efficacy of ASP2151, a helicase-primase inhibitor, against thymidine kinase-deficient herpes simplex virus type 2 infection in vitro and in vivo (2012)
  • Author(s): Takehiro Himaki, Yumi Masui, Koji Chono, Tohru Daikoku, Masaya Takemoto, Bo Haixia, Tomoko Okuda, Hiroshi Suzuki, Kimiyasu Shiraki
  • Journal Title: Antiviral Research Volume: 93-2 Pages: 301-4
  • Peer Reviewed
• [Journal Article] Efficacy of ASP2151, a helicase-primase inhibitor, against thymidine kinase-deficient herpes simplex virus type 2 infection in vitro and in vivo. (2012)
  • Author(s): Himaki T, Masui Y, Chono K, Daikoku T, Takemoto M, Haixia B, Okuda T, Suzuki H, Shiraki K.
  • Journal Title: Antiviral Research. Volume: 93(2) Issue: 2 Pages: 301-304
  • DOI: 10.1016/j.antiviral.2011.11.015
  • Peer Reviewed
• [Journal Article] Characterization of a fully human monoclonal antibody against extracellular domain of matrix protein 2 of influenza A virus (2011)
  • Author(s): Tatsuhiko Ozawa, Aishun Jin, Kazuto Tajiri, Masaya Takemoto, Tomoko Okuda, Kimiyasu Shiraki, Hiroyuki Kishi, Atsushi Muraguchi
  • Journal Title: Antiviral Research Volume: 91-3 Pages: 283-7
  • Peer Reviewed
• [Journal Article] Neutralizing anti-gH antibody of Varicella-zoster virus modulates distribution of gH and induces gene regulation, mimicking latency (2011)
  • Author(s): Kimiyasu Shiraki, Tohru Daikoku, Masaya Takemoto, Yoshihiro Yoshida, Kazuhiro Suzuki, Yasushi Akahori, Toshiomi Okuno, Yoshikazu Kurosawa, and Yoshizo Asano
  • Journal Title: Journal of Virology Volume: 85-16 Pages: 8172-80
  • Peer Reviewed
• [Journal Article] Neutralizing anti-gH antibody of Varicella-zoster virus modulates distribution of gH and induces gene regulation, mimicking latency (2011)
  • Author(s): Shiraki K, Daikoku T, Takemoto M, Yoshida Y, Suzuki K, Akahori Y, Okuno T, Kurosawa Y, Asano Y.
  • Journal Title: Journal of Virology Volume: 85(16) Issue: 16 Pages: 8172-8180
  • DOI: 10.1128/jvi.00435-11
  • Peer Reviewed
• [Journal Article] Polyphenols including catechin from green tea with in vitro antiviral activity exhibited anti-herpes simplex virus activity but not anti-influenza virus activity in mice (2011)
  • Author(s): Tohru Daikoku
  • Journal Title: Journal of Traditional Medicine Volume: 28 Pages: 63-72
  • Peer Reviewed
• [Journal Article] Novel anticytomegalovirus activity of immunosuppressant mizoribine and its synergism with ganciclovir (2010)
  • Author(s): Takashi Kuramoto, Tohru Daikoku, Yoshihiro Yoshida, Masaya Takemoto, Kumi Oshima, Yoshito Eizuru, Yoshinobu Kanda, Toshio Miyawaki and Kimiyasu Shiraki
  • Journal Title: Journal of Pharmacology and Experimental Therapeutics Volume: 333-3 Pages: 816-21
  • Peer Reviewed
• [Presentation] Aberrant VZV glycoproteins traffic modulated by neutralizing anti-gH MAb, International Union of Microbiological Societies 2011 Congress (2011)
  • Author(s): Masaya Takemoto, Tohru Daikoku, Kazuhiro Suzuki, Yasushi Akahori, Yoshikazu Kurosawa, Yoshizo Asano, Kimiyasu Shiraki
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2011-09-13
• [Presentation] Aberrant VZV glycoproteins traffic modulated by neutralizing anti-gH MAb (2011)
  • Author(s): Masaya Takemoto
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2011-09-13
• [Presentation] Aberrant VZV glycoproteins traffic modulated by neutralizing anti-gH MAb (2011)
  • Author(s): Masaya Takemoto, Tohru Daikoku, Kazuhiro Suzuki, Yasushi Akahori, Yoshikazu Kurosawa, Yoshizo Asano, Kimiyasu Shiraki
  • Organizer: 36th Annual International Herpesvirus Workshop
  • Place of Presentation: Gdansk, Poland
  • Year and Date: 2011-07-27
• [Presentation] Aberrant VZA glycoproteins traffic modulated by neutralizing anti-gH MAb (2011)
  • Author(s): Masaya Takemoto
  • Organizer: 36^<th> Annual International Herpesvirus Workshop
  • Place of Presentation: Gdansk, Poland
  • Year and Date: 2011-07-27
• [Presentation] 水痘・帯状疱疹ウイルスglycoprotein Hに対する中和抗体による、細胞内ウイルスの感染性消失過程の解析 (2010)
  • Author(s): 武本眞清,大黒徹,吉田与志博,浅野喜造,白木公康
  • Organizer: 第58回日本ウイルス学会
  • Place of Presentation: あわぎんホール(徳島)
  • Year and Date: 2010-11-09
• [Presentation] 水痘・帯状疱疹ウイルスglycoprotein Hに対する中和抗体による、細胞内ウイルスの感染性消失過程の解析 (2010)
  • Author(s): 武本眞清
  • Organizer: 日本ウイルス学会
  • Place of Presentation: あわぎんホール(徳島)
  • Year and Date: 2010-11-09
• [Presentation] Anti-gH neutralizing antibody alters localization of VZV glycorpoteins B and E (2010)
  • Author(s): Masaya Takemoto, Tohru Daikoku, Kazuhiro Suzuki, Yasushi Akahori, Yoshikazu Kurosawa, Yoshizo Asano, and Kimiyasu Shiraki
  • Organizer: 35th Annual International Herpesvirus Workshop
  • Place of Presentation: University of Utah, USA
  • Year and Date: 2010-07-26
• [Presentation] Anti-gH neutralizing antibody alters localization of VZV glycorpoteins B and E (2010)
  • Author(s): Masaya Takemoto
  • Organizer: International Herpesvirus Workshop
  • Place of Presentation: University of Utah, USA
  • Year and Date: 2010-07-26