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Analysis for the mechanism promoting HIV latent infection.

Research Project

Project/Area Number 22790430
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Virology
Research InstitutionKyoto University

Principal Investigator

EBINA Hirotaka  京都大学, ウイルス研究所, 助教 (60541951)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords潜伏感染 / HIV / インテグレーション / ヒト化マウス / エピジェネティクス
Research Abstract

Although combination antiretroviral therapy(cART) has reduced the pathogenesis of AIDS羊elated malignancies, it is still impossible to eliminate HIV from infected individual. The HIV latent infection is proposed to be a reason for the incurable phenomenon. However, the mechanism prompting latent infection in HIV infection is still unclear. To clarify the mechanism promoting HIV latency inviveo and in vitro, we performed several experiments.
2011, we found specific integration pathway that has a potential to induce latently infection. We found HIV cDNA can be inserted into the host chromosome even in the presence of integrase(IN) inhibitor. Moreover, we found that the HIV integration pattern is modified under IN-defient conditions and it may cause reduced promoter activity.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (22 results)

All 2012 2011 2010 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (12 results) Remarks (2 results)

  • [Journal Article] Integraseindependent HIV-1 infection is augmented under conditions of DNA damage and produces a viral reservoir.2012

    • Author(s)
      Ebina H, Kanemura Y, Suzuki Y, Urata K, Misawa N, Koyanagi Y.
    • Journal Title

      Virology

      Volume: 427(1) Pages: 44-50

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Integrase-independent HIV-1 infection is augmented under conditions of DNA damage and produces a viral reservoir2012

    • Author(s)
      Ebina H, Kanemura Y, Suzuki Y, Urata K, Misawa N, Koyanagi Y
    • Journal Title

      Virology

      Volume: 427 Issue: 1 Pages: 44-50

    • DOI

      10.1016/j.virol.2012.02.004

    • NAID

      120004007474

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The hematopoietic cell specific Rho GTPase inhibitor ARHGDIB/D4GDI limits HIV-1 replication2011

    • Author(s)
      Watanabe T., Urano E., Miyauchi K., Ichikawa R., Hamatake M., Misawa N., Sato K., Ebina H., Koyanagi Y. and Komano J
    • Journal Title

      AIDS Res. Hum. Retroviruses

      Volume: 85 Pages: 9726-9736

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] APOBEC1mediated editing and attenuation of herpes simplex virus 1 DNA indicate that neurons have an antiviral role during herpes simplex encephalitis2011

    • Author(s)
      Gee P., Ando Y., Kitayama H., YamamotoS. P., Kanemura Y.
    • Journal Title

      J. Virol.

      Volume: 85 Pages: 9726-9736

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of amino acids in the human tetherin trans membrane domainresponsible for HIV-1 VPU interaction and susceptibility2011

    • Author(s)
      Kobayashi T, Ode H, Koyanagi Y, 11人中11番目
    • Journal Title

      J Virol

      Volume: 85 Issue: 2 Pages: 932-945

    • DOI

      10.1128/jvi.01668-10

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rho GTPase inhibitorARHGDIB/D4GDI limits HIV-1 replication2011

    • Author(s)
      Watanabe T, Urano E, Koyanagi Y, KomanoJ, 10人中9番目
    • Journal Title

      AIDS Research and Human Retroviruses

      Volume: (印刷中) Issue: 8 Pages: 1-10

    • DOI

      10.1089/aid.2011.0180

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] APOBEC1-mediated editing and attenuation of herpes simplex virus 1 DNA indicate that neurons have an antiviral role during herpes simplex encephalitis2011

    • Author(s)
      Gee P, Ando Y, Kitayama H, Yamamoto SP, Kanemura Y, Ebina H, Kawaguchi Y, Koyanagi Y
    • Journal Title

      Journal of Virology

      Volume: 85 Issue: 19 Pages: 9726-9736

    • DOI

      10.1128/jvi.05288-11

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of amino acids in the human tetherin transmembrane domain responsible for HIV-1 Vpu interaction and susceptibility.2011

    • Author(s)
      Kobayashi T, Ode H, Yoshida T, Sato K, Gee P, Yamamoto SP, Ebina H, Strebel K, Sato H, Koyanagi Y.
    • Journal Title

      J Virol.

      Volume: 85 Pages: 932-945

    • NAID

      120002723154

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Integrase-independent HIV-1 infection is augmented under condition of DNA damage and produces a viral reservoir2011

    • Author(s)
      Ebina H., Kanemura Y., Suzuki Y., Urata K., Misawa N., Koyanagi
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2011-12-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] Construction of labeling method for nucleosomeformed viral DNA.2011

    • Author(s)
      Ebina H., Urata K., Kanemura Y. and Koyanagi Y
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-13
    • Related Report
      2011 Final Research Report
  • [Presentation] Construction of labeling method for nucleosome-formed viral DNA2011

    • Author(s)
      Ebina H., Urata K., Kanemura Y., Koyanagi Y.
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2011-12-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] APOBEC1-mediated editing and attenuation of HSV-1 UNA implicates an antiviral role in neurons during encephalitis2011

    • Author(s)
      Gee P., Ando Y., Kitayama H., Yammaoto S.P., Kanemura Y., Ebina H., Kawaguchi Y., Koyanagi Y.
    • Organizer
      第40回日本免疫学会
    • Place of Presentation
      幕張メッセ(千葉市)
    • Year and Date
      2011-11-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] Biochemical characterization of the HIV-1 restriction factor SAMHD12011

    • Author(s)
      Gee P., Okamoto S., Fukuhara M., Kanemura Y., Ebina H., Koyanagi Y.
    • Organizer
      12th Kumamoto AIDS Seminar-GCOE Joint International Symopsium
    • Place of Presentation
      阿蘇リゾートグランヴィリオホテル(阿蘇市)
    • Year and Date
      2011-10-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] HIV-I cDNA integration and persistent infection by DNA repair system.2011

    • Author(s)
      Ebina H., Kanemura Y., Suzuki Y., Urata K. and Koyanagi Y
    • Organizer
      XV International Congress of Virology
    • Place of Presentation
      札幌
    • Year and Date
      2011-09-15
    • Related Report
      2011 Final Research Report
  • [Presentation] HIV-1 cDNA integration and persistent infection by DNA repair system2011

    • Author(s)
      Ebina H., Kanemura Y., Suzuki Y., Urata K., Koyanagi Y.
    • Organizer
      XV International Congress of Virology
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Year and Date
      2011-09-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] The hematopoietic cell-specific Rho GTPase inhibitor ARHGDIB/D4GDI limits HIV-1 replication2011

    • Author(s)
      Watanabe T., Urano E., Miyauchi K., Ichikawa R., Hamatake M., Sato K., Hirotaka E., Koyanagi Y., Koraanao J.
    • Organizer
      XV International Congress of Virology
    • Place of Presentation
      札帆コンベンションセンター(札幌市)
    • Year and Date
      2011-09-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] Integrase independent retroviral cDNA integration, which is indefensible with integrase inhibitor2011

    • Author(s)
      Ebina H., Kanemura Y., Suzuki Y., Urata K. and Koyanagi Y
    • Organizer
      第6回研究所ネットワーク国際シンホシウム
    • Place of Presentation
      東京
    • Year and Date
      2011-06-09
    • Related Report
      2011 Final Research Report
  • [Presentation] Integrase independent retroviral cDNA integration, which is indefensible with integrase inhibitor2011

    • Author(s)
      Ebina H., Kanemura Y., Suzuki Y., Urata K., Koyanagi Y.
    • Organizer
      第6回研究所ネットワーク国際シンポジウム
    • Place of Presentation
      東京医科歯科大学(東京都)
    • Year and Date
      2011-06-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] 細胞内DNA修復システムによるレトロウイルスcDNA組込み機能の代償2010

    • Author(s)
      蝦名博貴、鈴木康嗣、金村優香、津村斐子、小柳義夫
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      ポートアイランド(神戸)
    • Year and Date
      2010-12-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] HIV cDNAのインテグラーゼ非依存性組込みとウイルス複製2010

    • Author(s)
      蝦名博貴、鈴木康嗣、金村優香、津村斐子、小柳義夫
    • Organizer
      第58回日本ウイルス学会学術集会
    • Place of Presentation
      あわぎんホール(徳島)
    • Year and Date
      2010-11-07
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.virus.kyoto-u.ac.jp/Lab/KoyanagiHP/saito/TOP.html

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.virus.kyoto-u.ac.jp/Lab/saito/TOP.html

    • Related Report
      2011 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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