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Circumvention of RNA decay in cells infected with SARS coronavirus

Research Project

Project/Area Number 22790431
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Virology
Research InstitutionOsaka University

Principal Investigator

KAMITANI Wataru  大阪大学, 微生物病研究所, 特任准教授 (60551421)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords分子 / RNA分解 / 翻訳阻害 / ウイルス / 感染症 / RNA
Research Abstract

SARS coronavirus nsp1 protein was shown to suppress host protein syntheses through the degradation of mRNA and the binding to 40S ribosomal subunits. However, SCoV is capable of replicating well in cells impaired the host protein syntheses by the expression of nsp1. To determine the mechanism of an efficient translation of mRNA of SCoV in cells expressing the nsp1, we investigated the effect of the UTRs of SCoV on the evasion from the nsp1-mediated translational suppression. We generated the reporter plasmids carrying a firefly luciferase gene flanked by the 5' UTR and/ or 3' UTR of SCoV and determined the luciferase activity in cells expressing the nsp1. Suppression of luciferase activity by the expression of nsp1 was cancelled by the transfection of the reporter plasmids carrying the 5' UTR of SCoV but not by those possessing the 3' UTR. Protein-RNA binding assay revealed that the positively charged region in the nsp1 responsible for a specific interaction with the stem-loop1 in the 5' UTR.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (9 results)

All 2011 2010

All Presentation (9 results)

  • [Presentation] Circumvention of the translational shut-off in cells infected with SARS coronavirus through the interaction of nsp1 with 5' UTR of viral mRNA2011

    • Author(s)
      Tomohisa TANAKA
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜・(神奈川)
    • Year and Date
      2011-12-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] Ciircumventionn of the translatioonal shut-off in ceells infectted with SARS corronavirus thhrough the interaction of nsp1 with 5' UTR of viral mRNA2011

    • Author(s)
      田中智久・松浦善治・神谷亘
    • Organizer
      第34回日日本分子生物学会年会
    • Place of Presentation
      横浜,日本
    • Year and Date
      2011-12-13
    • Related Report
      2011 Final Research Report
  • [Presentation] Circumvention of the translational shut-off in cells infected with SARS coronavirus through the interaction of nsp1 with 5' UTR of viral mRNA2011

    • Author(s)
      Tomohisa TANAKA
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      札幌コンベンションセンター・(北海道)
    • Year and Date
      2011-09-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] Circumvention of the translational shut-off in cells infected with SARS coronavirus through the interaction of nsp1 with 5' UTR of viral mRNA2011

    • Author(s)
      Tomohisa TANAKA
    • Organizer
      American Society for Virology 2011 The 31th annual meeting
    • Place of Presentation
      University of Minnesota (Minnesota, U.S.A.)
    • Year and Date
      2011-07-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] Circumvention of the translational shut-off in cells infected with SARS corronavirus thhrough the interaction of nsp1 with 5' UTR of viral mRNA2011

    • Author(s)
      田中智久・松浦善治・神谷亘
    • Organizer
      Intternational Union of Microbiological Soccieties 2011 Congress
    • Place of Presentation
      札幌,日本
    • Related Report
      2011 Final Research Report
  • [Presentation] Circumvention of the translational shut-off in cells infected with SARS corronavirus thhrough the interaction of nsp1 with 5' UTR of viral mRNA2011

    • Author(s)
      田中智久・松浦善治・神谷亘
    • Organizer
      Amerrican Socciety for Virology 2011 The 31th annnual meeet ing
    • Place of Presentation
      ミネアポリス,米国
    • Related Report
      2011 Final Research Report
  • [Presentation] SARS-CoVのnsp1タンパク質による翻訳阻害機構2010

    • Author(s)
      田中智久
    • Organizer
      第58回日本ウイルス学会
    • Place of Presentation
      あわぎんホール(徳島県)
    • Year and Date
      2010-11-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] SARS-CoVのnsp1タンパク質による翻訳阻害機構2010

    • Author(s)
      田中智久・松浦善治・神谷亘
    • Organizer
      第58回日本ウイルス学会学術集会
    • Place of Presentation
      徳島県,日本
    • Related Report
      2011 Final Research Report
  • [Presentation] SARS CORONAVIRUS NSP1 SUPPRESSES TRANSLATION BY BINDING TO 40S RIBOSOMAL SUBUNIT2010

    • Author(s)
      神谷亘・田中智久・Shinji MAKINO・松浦善治
    • Organizer
      第10回あわじしま感染症・免疫フォーラム
    • Place of Presentation
      兵庫県,日本
    • Related Report
      2011 Final Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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