Functional analysis of HIV-1 integrase that involves in various steps of viral replication
| Project/Area Number |
22790434
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
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| Research Institution | The University of Tokushima |
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Principal Investigator |
MIYAKE Ariko 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教 (20548622)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | ウイルス / HIV-1 / Pol-IN |
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| Research Abstract |
Analysis of adaptive mutations in macaque cell-tropic HIV-1 has shown that these alterations cluster in the C-terminal portion of integrase-coding region in the viral genome. Even a synonymous mutation was found to have a growth-enhancing effect. This effect was due to the enhanced expression of Gag and Gag-Pol proteins. Extensive analysis of the C-terminal integrase region has revealed that synonymous mutations generate both positive and negative effects on viral replication. It is thus evident that this integrase region modulates viral replication ability.
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Report
(3 results)
Research Products
(15 results)
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[Journal Article] Inhibition of active HIV-1 replication by NF-κB inhibitor DHMEQ2010
Author(s)
Miyake, A., Ishida, T., Yamagishi, M., Hara, T., Umezawa, K., Watanabe, T., and Horie, R.
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Journal Title
Microbes and Infection
Volume: 12
Pages: 400-408
Related Report
Peer Reviewed
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[Journal Article] Different interaction between HIV-1 Vif and its cellular target proteins APOBEC3G/ APOBEC3F2010
Author(s)
Nagao, T., Yamashita, T., Miyake, A., Uchiyama, T., Nomaguchi, M., and Adachi, A.
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Journal Title
Journal of Medical Investigation
Volume: 57
Pages: 89-94
NAID
Related Report
Peer Reviewed
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[Presentation] A novel antisense RNA of HIV-1 ale, functions as a self-limiting factor for the HIV-1 infection2011
Author(s)
Kobayashi-Ishihara, M., Yamagishi, M., Hara, T., Matsuda, Y., Miyake, A., Nakano, K., Ishida, T., Watanabe, T.
Organizer
XV International Congress of Virology
Place of Presentation
Sapporo Convention Center(Sapporo, Japan)
Year and Date
2011-09-13
Related Report
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[Presentation] Analysis of growth adaptive mutations in HIV-1 genome identifies a pol-integrase region that enhances virion production in a cell-independent and codon triplet-dependent manner2010
Author(s)
Miyake, A., Doi, N., Fujiwara, S., Adachi, A., and Nomaguchi, M.
Organizer
The 10th Awaji International Forum on Infection and Immunity
Place of Presentation
Awaji Yumebutai International Conference Center(Awaji, Japan)
Year and Date
2010-09-09
Related Report
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