| Project/Area Number |
22790435
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
|
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | バクテリオファージ / ワクチン / 自然免疫 / プリオン / 抗体 / ファージディスプレイ / 免疫 |
|---|
| Research Abstract |
Phage display library is powerful tool to develop various recombinant peptides or human antibodies applicable for revolutionary therapeutics. In addition to this feature, peptide-displaying phage clones induce mimotope-specific antibody responses in mice. In this study, we have established a human IgG1 antibody specific to s-form prion protein(PrP) but not to.-form PrP, PRB7 IgG. Employing a peptide-displaying phage library, the mimotope was determined to be at# 128-132 of human PrP. A single injection of peptide-displaying phage stimulate innate immune response and elicited a strong IgG response in mice, suggests a possible application of peptide-displaying phage clones as vaccine carriers for induction of therapeutic antibodies.
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