M13 bacteriophage-based vaccine design for prion.

• Project/Area Number: 22790435
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: Kagoshima University
• Principal Investigator: HASHIGUCHI Shuhei 鹿児島大学, 大学院・理工学研究科, 助教 (40295275)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: バクテリオファージ / ワクチン / 自然免疫 / プリオン / 抗体 / ファージディスプレイ / 免疫

Research Abstract

Phage display library is powerful tool to develop various recombinant peptides or human antibodies applicable for revolutionary therapeutics. In addition to this feature, peptide-displaying phage clones induce mimotope-specific antibody responses in mice. In this study, we have established a human IgG1 antibody specific to s-form prion protein(PrP) but not to.-form PrP, PRB7 IgG. Employing a peptide-displaying phage library, the mimotope was determined to be at# 128-132 of human PrP. A single injection of peptide-displaying phage stimulate innate immune response and elicited a strong IgG response in mice, suggests a possible application of peptide-displaying phage clones as vaccine carriers for induction of therapeutic antibodies.

Research Products

• [Journal Article] Direct evidence of generation and accumulation of β-sheet-rich prion protein in ScN2a cells de novo illuminated by human IgG1 antibody recognizing β-form but not α-form of prion protein (2012)
  • Author(s): Toshiya Kubota, Yuta Hamazoe, Shuhei Hashiguchi, Daisuke Ishibashi, Kazuyuki Akasaka, Noriyuki Nishida, Shigeru Katamine, Suehiro Sakaguchi, Ryota Kuroki, Toshihiro Nakashima, Kazuhisa Sugimura.
  • Journal Title: The Journal of biological chemistry Volume: 287巻 Issue: 17 Pages: 14023-14039
  • DOI: 10.1074/jbc.m111.318352
  • Peer Reviewed
• [Journal Article] Desensitization vaccine strategy using M13 phage whose g8p displays a B cell IgG epitope of Cry j 1 as a major allergen of Japanese cedar pollen (2012)
  • Author(s): Kai Satomi, Shuhei Hashiguchi, Kazuhisa Sugimura
  • Journal Title: Peptide Science 2011 Pages: 269-270
• [Journal Article] Desensitization vaccine strategy using M13 phage whose g8p displays a B cell IgG epitope of Cry j 1 as a major allergen of Japanese cedar pollen (2012)
  • Author(s): Kai S, Hashiguchi S, et al
  • Journal Title: Peptide Science 2011 Pages: 269-270
• [Journal Article] Immunological basis of M13 phage vaccine : Regulation under MyD88 and TLR9 signaling (2011)
  • Author(s): Shuhei Hashiguchi, Yuya Yamaguchi, Osamu Takeuchi, Shizuo Akira, Kazuhisa Sugimura
  • Journal Title: Biochemical and biophysical research communications Volume: 402巻 Issue: 1 Pages: 19-22
  • DOI: 10.1016/j.bbrc.2010.09.094
  • Peer Reviewed
• [Journal Article] Immunological basis of M13 phage vaccine : Regulation under MyD88 and TLR9 signaling. (2010)
  • Author(s): Hashiguchi S, Yamaguchi Y, Takeuchi O, Akira S, Sugimura K
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 402 Pages: 19-22
  • Peer Reviewed
• [Journal Article] Essential role of MyD88 in humoral response to M13 bacteriophage in mice and negative role of TLR9 in IgG1 response. (2010)
  • Author(s): Hashiguchi S, Yamaguchi Y, Takeuchi O, Akira S, Sugimura K.
  • Journal Title: International Immunology Volume: 22 : Supplement Number 1
• [Presentation] Strong Innate Immunity to M13 Bacteriophage : Implication for Vaccine Development (2011)
  • Author(s): Shuhei Hashiguchi, Takuma Gotanda, Yuta Hamazoe, Tsuyoshi Tsurumaru, Kazuhisa Sugimura
  • Organizer: 5th Vaccine and ISV Annual Global Congress
  • Place of Presentation: Seattle, USA
• [Presentation] Amyloid beta-42-mimotope displaying M13 bacteriophage for vaccine vehicle of Alzheimer's disease (2011)
  • Author(s): Kazuhisa Sugimura, Shuhei Hashiguchi, TEppei Osako, Sotaro Kawabata, Risa Abe, Shiro Nakagawa
  • Organizer: 5th Vaccine and ISV Annual Global Congress
  • Place of Presentation: Seattle, USA
• [Presentation] Monoclonal human IgG1 specific to Prion (2011)
  • Author(s): Toshiya Kubota, Yuta Hamazoe, Shuhei, Hashiguchi, Daisuke Ishibashi, Noriyuki Nishida, Suehiro Sakaguchi, Kazuhisa Sugimura
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
• [Presentation] Strong innate immunity to M13 bacteriophage : Implication for vaccine development (2011)
  • Author(s): Shuhei Hashiguchi, Takuma Gotanda, Hamazoe Yuta, Kazuhisa Sugimura
  • Organizer: 第40回日本免疫学会総会・学術集会記録
  • Place of Presentation: 千葉
• [Presentation] M13 bacteriophage for vaccine vehicle of Alzheimer's disease (2011)
  • Author(s): Kazuhisa Sugimura, Shuhei Hashiguchi, Teppei Osako, Kawabata Sotaro, Risa Abe
  • Organizer: 1st Oxford International bacteriophage conference, Phage2011 : Bacteriophage Application
  • Place of Presentation: Oxford, UK
• [Presentation] Monoclonal human IgG1 specific to Prion (2011)
  • Author(s): Kubota T, Hamazoe Y, Hashiguchi S, et al
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都市
• [Presentation] Strong innate immunity to M13 bacteriophage (2011)
  • Author(s): Hashiguchi S, Gotannda T, et al
  • Organizer: 第40回日本免疫学会総会・学術集会記録
  • Place of Presentation: 千葉市
• [Presentation] M13バクテリオファージ免疫におけるTLRファミリー分子の役割について (2010)
  • Author(s): 田原春菜、橋口周平、山口雄也、竹内理、審良静男、杉村和久
  • Organizer: BMB2010第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2010-12-09
• [Presentation] Immunological basis of M13 phage vaccines : an effective vaccine design tested in a mouse model of Alzheimer's disease. (2010)
  • Author(s): Hashiguchi S, Yamaguchi Y, Tahara H, Yamada T, Kai S, Abe R, Tanaka K, Sugimura K.
  • Organizer: 5th International Peptide Symposium
  • Place of Presentation: 京都府京都市
  • Year and Date: 2010-12-06
• [Presentation] Essential role of MyD88 in humoral response to M13 bacteriophage in mice and negative role of TLR9 in IgG1 response. (2010)
  • Author(s): Hashiguchi S, Yamaguchi Y, Takeuchi O, Akira S, Sugimura K.
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2010-08-24
• [Presentation] M13バクテリオファージが誘導するIgG抗体応答はMyD88に依存する (2010)
  • Author(s): 橋口周平、山口雄也、竹内理、審良静男、杉村和久
  • Organizer: 日本生化学会九州支部例会
  • Place of Presentation: 鹿児島県鹿児島市
  • Year and Date: 2010-05-22
• [Presentation] Immunological basis of M13 phage vaccines : an effective vaccine design tested in a mouse model of Alzheimer's disease (2010)
  • Author(s): Shuhei Hashiguchi, Yuya Yamaguchi, Haruna Tahara, Takashi Yamada, Satomi Kai, Risa Abe, Koichi Tanaka, Kazuhisa Sugimura
  • Organizer: 5th International Peptide Symposium
  • Place of Presentation: 京都
• [Presentation] M13バクテリオファージ免疫におけるTLRファミリー分子の役割について (2010)
  • Author(s): 田原春菜, 橋口周平, 山口雄也, 竹内理, 審良静男、杉村和久
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学大会合同大会
  • Place of Presentation: 神戸
• [Presentation] Essential role of MyD88 in humoral response to M13 bacteriophage in mice and negative role of TLR9 in IgG1 response (2010)
  • Author(s): Shuhei Hashiguchi, Yuya Yamaguchi, Osamu Takeuchi, Shizuo Akira, Kazuhisa Sugimura
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: 神戸
• [Presentation] M13バクテリオファージが誘導するIgG抗体応答はMyD88に依存する (2010)
  • Author(s): 橋口周平、山口雄也、竹内理, 審良静男、杉村和久
  • Organizer: 日本生化学会九州支部例会
  • Place of Presentation: 鹿児島
• [Book] 生化学:ファージディスプレイとBeyond antibody :～抗体様分子による分子標的～ (2010)
  • Author(s): 橋口周平, 伊東祐二, 田中孝一, 松木園美穂, 村岡賢, 杉村和久
  • Publisher: 社団法人日本生化学会
• [Book] 生化学:ファージディスプレイとBeyond antibody:-抗体様分子による分子標的- (2010)
  • Author(s): 橋口周平、伊東祐二、田中孝一、松木園美穂、村岡賢、杉村和久
  • Publisher: 社団法人日本生化学会
• [Remarks]
• [Remarks]
  • URL: http://www.be.kagoshima-u.ac.jp/~sugimura-lab/