Development of the methods for site-specific integration of LV vector with novel ZFP-LEDGF fusion protein

Research Project

Project/Area Number	22790436
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Virology
Research Institution	Yokohama City University
Principal Investigator	KONDO Asami 横浜市立大学, 医学部, 助教 (30453046)
Research Collaborator	RYO Akihide 横浜市立大学, 医学研究科, 教授 (20363814)
Project Period (FY)	2010 – 2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000) Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	レンチウイルスベクター / 配列特異的挿入 / 遺伝子ノックアウト
Research Abstract	Although lentiviral(LV) vector genomic integration step is essential for gene expression, mutations are found in host genome accompanied with this step. This aim of project is to develop of the methods for site-specific integration of LV vector. I fused the essential protein for integration of LV vector and DNA binding domain which was designed to bind sequence specific DNA. I could not find the sequence-specific integration of LV vector with these fusion proteins.

Report

(3 results)