Elucidation of molecular mechanisms HIV viral latency and its reactivation involving epigenetic regulation

• Project/Area Number: 22790437
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: Nihon University
• Principal Investigator: IMAI Kenichi 日本大学, 歯学部, 准教授 (60381810)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: HIV/AIDS / 潜伏感染 / 酪酸 / G9a / HDAC / エピジェネティクス / ヒストン / HIV / HDA / 転写 / エピジェネティック / メチル化

Research Abstract

Elucidation of the molecular mechanisms involved in the maintenance and breakdown of HIV latency is required for a comprehensive understanding of the pathophysiology of HIV-1 infection and the development of novel therapies. We have examined that epigenetic regulation is involved in the establishment and maintenance of HIV-1 latency and in the reactivation of HIV-1 by butyric acid-producing bacteria.

Research Products

• [Journal Article] Reactivation of latent HIV-1 by a wide variety of butyric acid-producing bacteria (2012)
  • Author(s): Imai K, Yamada K, Tamura M, Ochiai K, Okamoto T
  • Journal Title: Cell. Mol. Life Sci. Volume: (in press)
  • NAID: 10029976312
  • Peer Reviewed
• [Journal Article] Functional characterization of human cyclin T1 N-terminal region for human immunodeficiency virus-1 Tat transcriptional activation (2011)
  • Author(s): Asamitsu K, Hibi Y, Imai K, Victoriano AF, Kurimoto E, Kato K, Okamoto T
  • Journal Title: J Mol Biol. Volume: Vol.410(5) Pages: 887-895
  • Peer Reviewed
• [Journal Article] Novel histone deacetylase inhibitor NCH-51 activates latent HIV-1 gene expression (2011)
  • Author(s): Victoriano AF, Imai K, Togami H, Ueno T, Asamitsu K, Suzuki T, Miyata N, Ochiai K, Okamoto T
  • Journal Title: FEBS. L. Volume: Vol.685(7) Pages: 1103-1111
  • Peer Reviewed
• [Journal Article] The periodontal pathogen Porphyromonas gingivalis induces the Epstein-Barr Virus lytic switch transactivator ZEBRA by histone modification (2011)
  • Author(s): Imai K, et al
  • Journal Title: Biochimie Volume: 94(3) Pages: 839-846
  • NAID: 10031131378
  • Peer Reviewed
• [Journal Article] Butyric acid effects in the development of periodontitis and systemic diseases (2011)
  • Author(s): Ochiai K, et al
  • Journal Title: J.Oral science Volume: 53(3) Pages: 213-220
  • Peer Reviewed
• [Journal Article] Functional characterization of human cyclin T1 N-terminal region for human immunodeficiency virus-1 Tat transcriptional activation (2011)
  • Author(s): Asamitsu K, et al
  • Journal Title: J Mol Biol Volume: 410(5) Pages: 887-895
  • Peer Reviewed
• [Journal Article] Novel histone deacetylase inhibitor NCH-51 activates latent HIV-1 gene expression (2011)
  • Author(s): Victoriano AF, et al
  • Journal Title: FEBS.L Volume: 685(7) Pages: 1103-1111
  • Peer Reviewed
• [Journal Article] Involvement of histone H3 Lysine 9(H3K9) methyl transferase G9a in the maintenance of HIV-1 latency and its reactivation by BIX01294 (2010)
  • Author(s): Imai K, Togami H, Okamoto T
  • Journal Title: J Biol Chem. Volume: Vol.285(22) Pages: 16538-16545
  • Peer Reviewed
• [Journal Article] Involvement of histone H3 Lysine 9 (H3K9) methyl transferase G9a in the maintenance of HIV-1 latency and its reactivation by BIX01294. (2010)
  • Author(s): Imai K, et al.
  • Journal Title: J Biol.Chem. Volume: 285(22) Pages: 16538-16545
  • Peer Reviewed
• [Journal Article] Molecular mechanisms of HIV-1 latency and its breakdown by Periodontal diseases. (2010)
  • Author(s): Imai, K, et al
  • Journal Title: J.Oral Biosci. Volume: 52(3) Pages: 260-267
  • NAID: 10027106213
  • Peer Reviewed
• [Journal Article] Inhibition of inflammatory cytokine production from rheumatoid synovial fibroblasts by a novel IkappaB kinase inhibitor. (2010)
  • Author(s): Tsuchiya A, et al
  • Journal Title: J.Pharmacol.Exp.Ther. Volume: 333(1) Pages: 236-243
  • Peer Reviewed
• [Journal Article] Impaired plant growth and development caused by human immunodeficiency virus type 1 Tat. (2010)
  • Author(s): Cueno ME, et al
  • Journal Title: Transgenic Res. Volume: 19(5) Pages: 903-913
  • Peer Reviewed
• [Journal Article] Preferential expression and immunogenicity of HIV-1 Tat fusion protein expressed in tomato plant. (2010)
  • Author(s): Cueno ME, et al
  • Journal Title: Transgenic Res. Volume: 19(5) Pages: 889-895
  • Peer Reviewed
• [Presentation] Reactivation of latent HIV-1 infection by butyric acid-producing bacteria involves histone modification (2012)
  • Author(s): 今井健一
  • Organizer: International Union of Microbiological Societies 2011 & Congress concurrently held with the 15th International Congress of Virology held at Sapporo
  • Place of Presentation: Hokkaido, Japan
  • Year and Date: 2012-09-14
• [Presentation] HIV感染症における微生物間相互作用-酪酸産生常在菌による潜伏感染HIV-1の再活性化- (2012)
  • Author(s): 今井健一
  • Organizer: 第85回日本細菌学会総
  • Place of Presentation: 長崎ブリックホール,長崎
  • Year and Date: 2012-03-29
• [Presentation] HIV感染症における微生物間相互作用-酪酸産生常在菌による潜伏感染HIV-1の再活性化- (2012)
  • Author(s): 今井健一, 他
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎ブリックホール,(長崎県)
  • Year and Date: 2012-03-29
• [Presentation] 歯周病原細菌代謝産物・酪酸の微生物間相互作用-潜伏感染ウイルスの再活性化とガン進展に及ぼす影響 (2012)
  • Author(s): 落合邦康, 他
  • Organizer: 第23回日本臨床微生物学会総会
  • Place of Presentation: パシフィコ横浜,(神奈川県)(招待講演)
  • Year and Date: 2012-01-21
• [Presentation] 歯周病原細菌による潜伏感染EBVの再活性化と病態への関与 (2012)
  • Author(s): 今井健一
  • Organizer: 第45回日本無菌生物ノートバイオロジー学会総会
  • Place of Presentation: グランデール岐山,岐阜
  • Year and Date: 2012-01-13
• [Presentation] 歯周病原細菌による潜伏感染EBVの再活性化と病態への関与 (2012)
  • Author(s): 今井健一, 他
  • Organizer: 第45回日本無菌生物ノートバイオロジー学会総会
  • Place of Presentation: グランデール岐山,(岐阜県)
  • Year and Date: 2012-01-13
• [Presentation] Reactivation of latent HIV-1 by a wide variety of butyric acid-producing bacteria (2011)
  • Author(s): 今井健一
  • Organizer: The joint meeting the 17th international symposium on gnotobiology and the 34th congress of the society for microbial Ecology and disease
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2011-11-22
• [Presentation] 歯周病原菌の酸化ストレス応答に及ぼすHIV Tatの影響 (2011)
  • Author(s): Cueno ME, 他
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 長良川国際会議場,(岐阜県)
  • Year and Date: 2011-10-01
• [Presentation] ウイルス感染症における口腔細菌学の影響.歯周病原菌による潜伏感染EBVの再活性化機構 (2011)
  • Author(s): 今井健一
  • Organizer: 第53回歯科基礎医学会学術大会ならびに総会
  • Place of Presentation: 長良川国際会議場,岐阜
  • Year and Date: 2011-09-30
• [Presentation] ウイルス感染症における口腔細菌学の影響-歯周病原菌による潜伏感染EBVの再活性化機構- (2011)
  • Author(s): 今井健一, 他
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 長良川国際会議場,(岐阜県)(招待講演)
  • Year and Date: 2011-09-30
• [Presentation] Reactivation of latent HIV-1 infection by butyric acid-producing bacteria involves histone modification (2011)
  • Author(s): 今井健一, 他
  • Organizer: International Union of Microbiological Societies 2011 & Congress concurrently held with the 15th International Congress of Virology held at Sapporo
  • Place of Presentation: Hokkaido, Japan
  • Year and Date: 2011-09-15
• [Presentation] Human immunodeficiency virus-1 Tat induces oxidative stress in tomato plants (2011)
  • Author(s): Cueno ME, 他
  • Organizer: International Union of Microbiological Societies 2011 & Congress concurrently held with the 15th International Congress of Virology held at Sapporo
  • Place of Presentation: Hokkaido, Japan
  • Year and Date: 2011-09-13
• [Presentation] 感染症における微生物間相互作用-酪酸産生常在菌による潜伏感染HIV-1の再活性化- (2010)
  • Author(s): 今井健一, 他
  • Organizer: 第93回 日本細菌学会関東支部総会
  • Place of Presentation: 京王プラザホテル(東京)
  • Year and Date: 2010-10-22
• [Presentation] HIV感染症における微生物間相互作用-酪酸産生常在菌による潜伏感染HIV-1の性化- (2010)
  • Author(s): 今井健一, 他
  • Organizer: 第58回日本ウイルス学会学術集会・総会
  • Place of Presentation: 徳島県郷土文化会館
  • Year and Date: 2010-10-09
• [Presentation] 口腔感染症を誘因とする新たな難治性疾患第 (2010)
  • Author(s): 落合邦康, 他
  • Organizer: 52回 歯科基礎医学会学術大会ならびに総会
  • Place of Presentation: タワーホール船堀
  • Year and Date: 2010-09-21
• [Presentation] 酪酸産生菌による潜伏感染HIV-1の再活性化 (2010)
  • Author(s): 今井健一, 他
  • Organizer: 第52回歯科基礎医学会学術大会ならびに総会
  • Place of Presentation: ワーホール船堀(東京)
  • Year and Date: 2010-09-21
• [Presentation] Effects of butyric acid-producing bacteria on reactivation of latent HIV-1 (2010)
  • Author(s): Imai, K, et al
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2010-08-26
• [Presentation] 腸内細菌代謝産物による潜伏感HIV-1再活性化 (2010)
  • Author(s): 落合邦康, 他
  • Organizer: 第14回腸内細菌学会
  • Place of Presentation: 京都大学百周年記念ホール
  • Year and Date: 2010-06-17
• [Presentation] Reactivation of latent HIV-1 infection by butyric acid-producing bacteria involves hismodification (2010)
  • Author(s): Imai, K, et al
  • Organizer: 5th German-Japanese HIV-Symposium
  • Place of Presentation: Keio Univ., Tokyo
  • Year and Date: 2010-05-10
• [Remarks] ホームページアドレス
  • URL: http://www.dent.nihon-u.ac.jp/bact/index.html