Visualization of influenza virus progeny vRNP complex and analysis of cytoplasmic machineries responsible for polarized vRNP trafficking.

• Project/Area Number: 22790438
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: Kitasato University
• Principal Investigator: MOMOSE Fumitaka 北里大学, 大学院・感染制御科学府, 講師 (90332204)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: インフルエンザウイルス / 微小管 / 宿主因子 / モノクローナル抗体 / ライブセルイメージング / 低分子量GTP結合タンパク質 / リサイクリングエンドソーム / RNP複合体 / タンパク質導入 / 微小管輸送 / 極性輸送

Research Abstract

This study revealed that Rab11-positive recycling endosomes(RE) participate in the polarized trafficking of influenza A virus ribonucleoprotein complexes(vRNP) to the apical plasma membrane. The targeting of vRNP to the RE depends on the specific interaction between viral RNA dependent RNA polymerase on vRNP and GTP-bound active Rab11.This RE dependent apical transport of vRNP also requires class II Rab11 effector proteins such as Rab11-FIP3/4.

Research Products

• [Journal Article] Epitope mapping of neutralizing monoclonal antibody in avian influenza A H5N1 virus hemagglutinin (2012)
  • Author(s): Ohkura T, Kikuchi Y, Kono N, Itamura S, Komase K, Momose F, Morikawa Y
  • Journal Title: Biochem.Bioph.Res.Co. Volume: 418 Issue: 1 Pages: 38-43
  • DOI: 10.1016/j.bbrc.2011.12.108
  • Peer Reviewed
• [Journal Article] Apical transport of influenza A virus ribonucleoprotein requires Rab11-positive recycling endosome (2011)
  • Author(s): Momose F., et al.
  • Journal Title: PLoS ONE Volume: 6 Issue: 6 Pages: e21123-e21123
  • DOI: 10.1371/journal.pone.0021123
  • Peer Reviewed
• [Journal Article] Replication-coupled and host factor-mediated encapsidation of the influenza virus genome by viral nucleoprotein (2011)
  • Author(s): Kawaguchi A., et al
  • Journal Title: J.Virol. Volume: 85 Issue: 13 Pages: 6197-6204
  • DOI: 10.1128/jvi.00277-11
  • Peer Reviewed
• [Journal Article] Apical transport of influenza A virus ribonucleoprotein requires Rab11-positive recycling endosome (2011)
  • Author(s): Fumitaka Momose, et al
  • Journal Title: PLoS ONE Volume: 6
  • Peer Reviewed
• [Journal Article] Involvement of vesicular trafficking system in membrane targeting of the progeny influenza virus genome (2010)
  • Author(s): Shuichi Jo, Atsushi Kawaguchi, Naoki Takizawa, Yuko Morikawa, Fumitaka Momose, Kyosuke Nagata
  • Journal Title: Microbes Infect Volume: 12巻 Issue: 12-13 Pages: 1079-1084
  • DOI: 10.1016/j.micinf.2010.06.011
  • NAID: 120007137839
  • Peer Reviewed
• [Journal Article] Involvement of vesicular trafficking system in membrane targeting of the progeny influenza virus genome (2010)
  • Author(s): Jo S, Kawaguchi A, Takizawa N, Morikawa Y, Momose F, Nagata K.
  • Journal Title: Microbes and Infection Volume: 12 Pages: 1079-1084
  • NAID: 120007137839
  • Peer Reviewed
• [Presentation] Rab11陽性リサイクリングエンドソームによるインフルエンザウイルスゲノム-タンパク質複合体の極性輸送 (2011)
  • Author(s): 百瀬文隆, 関本哲也, 大倉喬, 森川裕子
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市
  • Year and Date: 2011-12-15
• [Presentation] Rab11陽性リサイクリングエンドソームによるインフルエンザウイルスゲノムータンパク質複合体の極性輸送 (2011)
  • Author(s): 百瀬文隆, 他
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-15
• [Presentation] Apical transport of influenza a virus ribonucleoprotein requires Rab11-positive recycling endosome (2011)
  • Author(s): Fumitaka Momose, Tetsuya Sekimoto, Takashi Ohkura, Shuichi Jo, Atsushi Kawaguchi, Kyosuke Nagata, Yuko Morikawa
  • Organizer: 15th International Congress of Virology
  • Place of Presentation: 札幌市
  • Year and Date: 2011-09-13
• [Presentation] Epitope mapping of neutralizing antibody in avian influenza A H5N1 virus hemagglutinin and construction of its single-chain variable fragment (2011)
  • Author(s): Takashi Ohkura, Yuji Kikuchi, Naoko Kono, Shigeyuki Itamura, Katsuhiro Komase, Fumitaka Momose, Yuko Morikawa
  • Organizer: 15th International Congress of Virology
  • Place of Presentation: 札幌市
  • Year and Date: 2011-09-13
• [Presentation] Apical transport of influenza a virus ribonucleoprotein requires Rab11-positive recycling endosome (2011)
  • Author(s): Fumitaka Momose, et al
  • Organizer: 15th International Congress of Virology
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2011-09-13
• [Presentation] Epitope mapping of neutralizing antibody in avian influenza A H5N1 virus hemagglutinin and construction of its single-chain variable fragment (2011)
  • Author(s): Takashi Ohkura, et al
  • Organizer: 15th International Congress of Virology
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2011-09-13
• [Presentation] インフルエンザウイルス子孫vRNP複合体の極性輸送を担う輸送小胞および宿主因子の同定 (2010)
  • Author(s): 百瀬文隆, 関本哲也, 大倉喬, 森川裕子
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-07
• [Presentation] インフルエンザウイルス子孫vRNP複合体の極性輸送を担う輸送小胞および宿主因子の同定 (2010)
  • Author(s): 百瀬文隆、関本哲也、大倉喬、森川裕子
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸国際展示場(兵庫)
  • Year and Date: 2010-12-07
• [Presentation] インフルエンザウイルス子孫vRNP複合体の極性輸送を担う輸送小胞および宿主因子の探索 (2010)
  • Author(s): 百瀬文隆, 大倉喬, 森川裕子
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島市
  • Year and Date: 2010-11-08
• [Presentation] インフルエンザウイルス子孫vRNP複合体の極性輸送を担う輸送小胞および宿主因子の探索 (2010)
  • Author(s): 百瀬文隆、大倉喬、森川裕子
  • Organizer: 第58回 日本ウイルス学会学術集会
  • Place of Presentation: あわぎんホール(徳島)
  • Year and Date: 2010-11-08
• [Presentation] インフルエンザウイルスRNAゲノム-タンパク質複合体の可視化による細胞質内輸送機構の解析 (2010)
  • Author(s): 百瀬文隆
  • Organizer: 第58回日本ウイルス学会学術集会シンポジウム
  • Place of Presentation: 徳島市
  • Year and Date: 2010-11-07
• [Presentation] インフルエンザウイルスRNAゲノム-タンパク質複合体の可視化による細胞質内輸送機構の解析 (2010)
  • Author(s): 百瀬文隆
  • Organizer: 第58回 日本ウイルス学会学術集会
  • Place of Presentation: あわぎんホール(徳島)(シンポジウム、招待講演)
  • Year and Date: 2010-11-07
• [Presentation] Live-cell imaging of the cytoplasmic trafficking of progeny influenza viral ribonucleoprotein complexes (2010)
  • Author(s): Fumitaka Momose, Tetsuya Sekimoto, Yuko Morikawa
  • Organizer: 14th International Conference on Negative Strand Viruses
  • Place of Presentation: Bruges, Belgium
  • Year and Date: 2010-06-21
• [Presentation] Live-cell imaging of the cytoplasmic trafficking of progeny influenza viral ribonucleoprotein complexes. (2010)
  • Author(s): Fumitaka Momose, Tetsuya Sekimoto, Yuko Morikawa
  • Organizer: 14th International Conference on Negative Strand Viruses
  • Place of Presentation: Bruges, Belgium
  • Year and Date: 2010-06-21
• [Remarks]
  • URL: http://www.kitasato-u.ac.jp/lisci/