Reverse genetics approach to study the molecular biology of rotavirus

• Project/Area Number: 22790440
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: Fujita Health University
• Principal Investigator: KOMOTO Satoshi 藤田保健衛生大学, 医学部, 講師 (60367711)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: ロタウイルス / ウイルス工学 / リバースジェネティクス / スパイク蛋白質 / VP4

Research Abstract

We generated and characterized a recombinant rotavirus expressing cDNA-derived VP4 with a modified cleavage site recognized by furin as well as trypsin. Unexpectedly, the VP4 cleavage site mutant virus could not undergo multicycle replication without an exogenous protease. Furthermore, the mutant virus showed lower infectivity even in the presence of trypsin compared with the parental virus carrying authentic VP4.These results suggest that intracellular cleavage of VP4 by furin may be disadvantageous for rotavirus infectivity.

Research Products

• [Journal Article] Modification of the trypsin cleavage site of rotavirus VP4 to a furin-sensitive form does not enhance replication efficiency (2011)
  • Author(s): Komoto, S., Wakuda, M., Ide, T., Niimi, G., Maeno, Y., Higo-Moriguchi, K., and Taniguchi, K.
  • Journal Title: J. Gen. Virol Volume: 92 Pages: 2914-2921
  • Peer Reviewed
• [Journal Article] Modification of the trypsin cleavage site of rotavirus VP4 to a furin-sensitive form does not enhance replication efficiency (2011)
  • Author(s): Satoshi Komoto
  • Journal Title: J.Gen.Virol. Volume: 92 Pages: 2914-2921
  • Peer Reviewed
• [Journal Article] Sudden death from systemic rotavirus infection and detection of nonstructural rotavirus proteins (2011)
  • Author(s): Ineko Nakano
  • Journal Title: J.Clin.Microbiol. Volume: 49 Pages: 4382-4385
  • Peer Reviewed
• [Journal Article] Porcine rotavirus closely related to novel group of human rotaviruses (2011)
  • Author(s): Mitsutaka Wakuda
  • Journal Title: Emerg.Infect.Dis. Volume: 17 Pages: 1491-1493
  • Peer Reviewed
• [Journal Article] Molecular and immunohistochemical detection of rotavirus in urinary sediment cells of children with rotavirus gastroenteritis (2011)
  • Author(s): Tadafumi Yokoyama
  • Journal Title: Clin.Microbiol.Infect. Volume: 17 Pages: 1190-1193
  • Peer Reviewed
• [Journal Article] Rotavirus nonstructural protein 1 suppresses virus-induced cellular apoptosis to facilitate viral growth by activating the cell survival pathways during early stages of infection. (2010)
  • Author(s): Parikshit Bagchi
  • Journal Title: Journal of Virology Volume: 84 Pages: 6834-6845
  • Peer Reviewed
• [Presentation] Modification of the trypsin cleavage site of rotavirus VP4 to a furin-sensitive form does not enhance replication efficiency (2011)
  • Author(s): Komoto, S., and Taniguchi, K.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 神奈川
  • Year and Date: 2011-12-14
• [Presentation] Modification of the trypsin cleavage site of rotavirus VP4 to a furin-sensitive form does not enhance replication efficiency (2011)
  • Author(s): Satoshi Komoto
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-14
• [Presentation] Modification of the trypsin cleavage site of rotavirus VP4 to furin-sensitive form does not enhance replication efficiency (2011)
  • Author(s): Komoto, S., Wakuda, M., Maeno, Y., Yui, A., Higo-Moriguchi, K., Sasaki, J., Ishikawa, K., and Taniguchi, K.
  • Organizer: XV International Congress of Virology
  • Place of Presentation: Hokkaido, Japan
  • Year and Date: 2011-09-13
• [Presentation] MODIFICATION OF THE TRYPSIN CLEAVAGE SITE OF ROTAVIRUS VP4 TO FURIN-SENSITIVE DOES NOT ENHANCE REPLICATION EFFICIENCY (2011)
  • Author(s): Satoshi Komoto
  • Organizer: XV International Congress of Virology
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2011-09-13
• [Presentation] Modification of the trypsin cleavage site of rotavirus VP4 to be recognized by furin does not result in enhanced replication (2010)
  • Author(s): Komoto, S., and Taniguchi, K.
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会合同大会
  • Place of Presentation: 神奈川
  • Year and Date: 2010-12-07
• [Presentation] Modification of the trypsin cleavage site of rotavirus VP4 to be recognized by furin does not result in enhanced replication (2010)
  • Author(s): Satoshi Komota
  • Organizer: 第33回日本分子生物学会・第83回日本生化学会合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2010-12-07
• [Presentation] VP4上のトリプシン切断領域にフューリン認識配列を導入した組換えロタウイルスの作製 (2010)
  • Author(s): 河本聡志、和久田光毅、前野芳正、油井晶子、佐々木潤、守口匡子、石川球美子、谷口孝喜
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
  • Year and Date: 2010-11-07
• [Presentation] VP4上のトリプシン切断領域にフューリン認織配列を導入した組換えロタウイルスの作製 (2010)
  • Author(s): 河本聡志
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: あわぎんホール(徳島県)
  • Year and Date: 2010-11-07
• [Presentation] Modification of the trypsin cleavage site of rotavirus VP4 to be recognized by furin does not result in enhanced replication (2010)
  • Author(s): Komoto, S., and Taniguchi, K.
  • Organizer: The 10th Awaji International Forum on Infection and Immunity
  • Place of Presentation: Hyogo, Japan
  • Year and Date: 2010-09-09
• [Presentation] Modification of the trypsin cleavage site of rotavirus VP4 to be recogaized by furin does not result in enhanced replication (2010)
  • Author(s): Satoshi Komoto
  • Organizer: The 10th Awaji International Forum on Infection and Immunity
  • Place of Presentation: 淡路夢舞台国際会議場(兵庫県)
  • Year and Date: 2010-09-09
• [Book] ロタウイルス胃腸炎の予防と治療の新しい展開 (2012)
  • Author(s): 河本聡志、谷口孝喜
  • Total Pages: 136
  • Publisher: 医薬ジャーナル社
• [Remarks]
  • URL: http://www.fujita-hu.ac.jp/~virology/index.htm
• [Remarks]
  • URL: http://www.fujita-hu.ac.jp/~virology/index.htm