Reverse genetics approach to study the molecular biology of rotavirus
Project/Area Number |
22790440
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Virology
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Research Institution | Fujita Health University |
Principal Investigator |
KOMOTO Satoshi 藤田保健衛生大学, 医学部, 講師 (60367711)
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Project Period (FY) |
2010 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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Keywords | ロタウイルス / ウイルス工学 / リバースジェネティクス / スパイク蛋白質 / VP4 |
Research Abstract |
We generated and characterized a recombinant rotavirus expressing cDNA-derived VP4 with a modified cleavage site recognized by furin as well as trypsin. Unexpectedly, the VP4 cleavage site mutant virus could not undergo multicycle replication without an exogenous protease. Furthermore, the mutant virus showed lower infectivity even in the presence of trypsin compared with the parental virus carrying authentic VP4.These results suggest that intracellular cleavage of VP4 by furin may be disadvantageous for rotavirus infectivity.
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Report
(3 results)
Research Products
(19 results)
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[Journal Article] Modification of the trypsin cleavage site of rotavirus VP4 to a furin-sensitive form does not enhance replication efficiency2011
Author(s)
Komoto, S., Wakuda, M., Ide, T., Niimi, G., Maeno, Y., Higo-Moriguchi, K., and Taniguchi, K.
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Journal Title
J. Gen. Virol
Volume: 92
Pages: 2914-2921
Related Report
Peer Reviewed
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[Presentation] Modification of the trypsin cleavage site of rotavirus VP4 to furin-sensitive form does not enhance replication efficiency2011
Author(s)
Komoto, S., Wakuda, M., Maeno, Y., Yui, A., Higo-Moriguchi, K., Sasaki, J., Ishikawa, K., and Taniguchi, K.
Organizer
XV International Congress of Virology
Place of Presentation
Hokkaido, Japan
Year and Date
2011-09-13
Related Report
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