Identification and application of human brain genes that facilitate JC virus replication.

• Project/Area Number: 22790446
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: NAKAMICHI Kazuo 国立感染症研究所, ウイルス第一部, 主任研究官 (50348190)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ウイルス / 脳 / 神経 / 脳神経疾患 / 神経ウイルス / 脳・神経

Research Abstract

JC virus is the causative agent of progressive multifocal leukoencephalopathy(PML), a fatal demyelinating disease in humans. Replicative efficacy of JCV in cultured cells is limited, which hampers in vitro studies including drug discovery. In this study, experimental systems were developed for the identification of human brain genes that facilitates JCV replication and for the establishment of JCV-permissive cell lines.

Research Products

• [Journal Article] Sequential changes in the non-coding control region sequences of JC polyomaviruses from the cerebrospinal fluid of patients with progressive multifocal leukoencephalopathy. (2013)
  • Author(s): Nakamichi K, Kishida S, Tanaka K, Suganuma A, Sano Y, Sano H, Kanda T, Maeda N, Kira JI, Itoh A, Kato N, Tomimoto H, Kurane I, Lim CK, Mizusawa H, Saijo M
  • Journal Title: Archives of Virology Volume: 158 Issue: 3 Pages: 639-650
  • DOI: 10.1007/s00705-012-1532-3
  • Peer Reviewed
• [Journal Article] Characteristics of progressive multifocal leukoencephalopathy clarified through internet-assisted laboratory surveillance in Japan. (2012)
  • Author(s): Nakamichi K, Mizusawa H, Yamada M, Kishida S, Miura Y, Shimokawa T, Takasaki T, Lim CK, Kurane I, Saijo M.
  • Journal Title: BMC Neurology Volume: 12 Issue: 1 Pages: 121-121
  • DOI: 10.1186/1471-2377-12-121
  • Peer Reviewed
• [Journal Article] Evaluation of a quantitative real-time PCR assay for the detection of JC polyomavirus DNA in cerebrospinal fluid without nucleic acid extraction. (2011)
  • Author(s): Nakamichi K, Kurane I, Saijo M
  • Journal Title: Japanese Journal of Infectious Diseases Volume: 64 Pages: 211-216
  • NAID: 40018820935
  • Peer Reviewed
• [Journal Article] Evaluation of a Quantitative Real-Time PCR Assay for the Detection of JC Polyomavirus DNA in Cerebrospinal Fluid without Nucleic Acid Extraction (2011)
  • Author(s): Kazuo Nakamichi(中道一生)
  • Journal Title: Japanese Journal of Infectious Diseases Volume: 64 Pages: 211-216
  • NAID: 40018820935
  • Peer Reviewed
• [Journal Article] Celastrol suppresses morphological and transcriptional responses in microglial cells upon stimulation with double-stranded RNA. (2010)
  • Author(s): Kazuo Nakamichi (中道一生)
  • Journal Title: International Journal of Neuroscience Volume: 120 Pages: 252-257
  • Peer Reviewed
• [Journal Article] Long-term infection of adult mice with murine polyomavirus following stereotaxic inoculation into the brain. (2010)
  • Author(s): Kazuo Nakamichi (中道一生)
  • Journal Title: Microbiology and Immunology Volume: 54 Pages: 475-482
  • NAID: 10029545429
  • Peer Reviewed
• [Presentation] 進行性多巣性白質脳症患者の脳脊髄液中に検出されたJCポリオーマウイルスの経時的なゲノム変異パターンの解析 (2012)
  • Author(s): 中道一生、林昌宏、西條政幸
  • Organizer: 第60回 日本ウイルス学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2012-11-15
• [Presentation] 進行性多巣性白質脳症患者の脳脊髄液中に検出されたJCポリオーマウイルスの経時的なゲノム変異パターンの解析 (2012)
  • Author(s): 中道一生
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪国際会議場（大阪府）
• [Presentation] 進行性多巣性白質脳症患者の脳脊髄液中に出現するJCポリオーマウイルスゲノムの転写調節領域における変異パターンの解析 (2011)
  • Author(s): 中道一生、林昌宏、倉根一郎、西條政幸
  • Organizer: 第16回日本神経感染症学会学術集会
  • Place of Presentation: 学術総合センター(東京都)
  • Year and Date: 2011-11-04
• [Presentation] Detection of JC polyomavirus DNA in cerebrospinal fluids collected from patients suspected as having progressive multifocal leukoencephalopathy in Japan (2011)
  • Author(s): Kazuo Nakamichi(中道一生)
  • Organizer: International Union of Microbiological Societies (IUMS) 2011 Congress
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2011-09-15
• [Presentation] 進行性多巣性白質脳症が疑われた血液疾患患者の脳脊髄液におけるJCポリオーマウイルスゲノムDNAの検出 (2010)
  • Author(s): 中道一生、伊藤(高山)睦代、倉根一郎、西條政幸
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島県郷土文化会館(徳島県)
  • Year and Date: 2010-11-08
• [Presentation] 脳脊髄液中のJCボリオーマウイルスの検査支援を介した日本国内における進行性多巣性白質脳症(PML)の発生状況の解析 (2010)
  • Author(s): 中道一生
  • Organizer: 第15回日本神経感染症学会
  • Place of Presentation: 福島ビューホテル(福島県)
  • Year and Date: 2010-10-08
• [Presentation] 定量的リアルタイムPCRによる脳脊髄液中JCウイルスゲノムの検出に基づく進行性多巣性白質脳症の診断支援 (2010)
  • Author(s): 中道一生
  • Organizer: 第84回日本感染症学会総会学術集会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2010-04-06