Identification and characterization of factors that regulate EB virus reactivation from latency

• Project/Area Number: 22790448
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: Aichi Cancer Center Research Institute
• Principal Investigator: MURATA Takayuki 愛知県がんセンター(研究所), 腫瘍ウイルス学部, 研究員 (30470165)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: EBウイルス / 再活性化 / ウイルス / 癌 / 低酸素 / SUMO化

Research Abstract

Epstein-Barr(EB) virus is a causative agent of serious diseases, including infectious mononucleosis, Burkitt's lymphoma, nasopharyngeal cancer, or gastric cancer. Changes of viral lifecycles, such as reactivation from latency, have been implicated in the severity or state of the diseases, and thus analyzing mechanisms of such transitions is of importance. We here newly identified transcription factors that regulate the virus reactivation, and observed epigenetic alterations mediated by those factors(Murata et al., JBC 2010, 2011, Murata et al., JV 2012).

Research Products

• [Journal Article] Epigenetic Histone Modification of Epstein-Barr Virus BZLF1 Promoter during Latency and Reactivation in Raji Cells (2012)
  • Author(s): Murata, T., Kondo, Y., Sugimoto, A., Kawashima, D., Saito, S., Isomura, H., Kanda, T., and Tsurumi, T.
  • Journal Title: J Virol Volume: 86(9) Pages: 4752-61
  • Peer Reviewed
• [Journal Article] Epigenetic Histone Modification of Epstein-Barr Virus BZLF1 Promoter during Latency and Reactivation in Raji Cells (2012)
  • Author(s): Murata T
  • Journal Title: Journal of Virology Volume: 86 Pages: 4752-61
  • Peer Reviewed
• [Journal Article] Unexpected Instability of Family of Repeats(FR), the Critical cis-Acting Sequence Required for EBV Latent Infection, in EBV-BAC Systems (2011)
  • Author(s): Kanda, T., Shibata, S., Saito, S., Murata, T., Isomura, H., Yoshiyama, H., Takada, K., and Tsurumi, T.
  • Journal Title: PLoS One Volume: 6(11)
  • Peer Reviewed
• [Journal Article] Identification and Characterization of CCAAT Enhancer-binding Protein(C/EBP) as a Transcriptional Activator for Epstein-Barr Virus Oncogene Latent Membrane Protein 1 (2011)
  • Author(s): Noda, C., Murata, T., Kanda, T., Yoshiyama, H., Sugimoto, A., Kawashima, D., Saito, S., Isomura, H., and Tsurumi, T
  • Journal Title: J Biol Chem Volume: 286(49) Pages: 42524-33
  • Peer Reviewed
• [Journal Article] Involvement of Jun Dimerization Protein 2(JDP2) in the Maintenance of Epstein-Barr Virus Latency (2011)
  • Author(s): Murata, T., Noda, C., Saito, S., Kawashima, D., Sugimoto, A., Isomura, H., Kanda, T., Yokoyama, K. K., and Tsurumi, T
  • Journal Title: J Biol Chem Volume: 286(25) Pages: 22007-16
  • Peer Reviewed
• [Journal Article] The Human Cytomegalovirus Gene Products Essential for Late Viral Gene Expression Assemble into Prereplication Complexes before Viral DNA Replication (2011)
  • Author(s): Isomura, H., Stinski, M. F., Murata, T., Yamashita, Y., Kanda, T., Toyokuni, S., and Tsurumi, T
  • Journal Title: J Virol Volume: 85(13) Pages: 6629-44
  • Peer Reviewed
• [Journal Article] Spatiotemporally different DNA repair systems participate in epstein-barr virus genome maturation (2011)
  • Author(s): Sugimoto, A., Kanda, T., Yamashita, Y., Murata, T., Saito, S., Kawashima, D., Isomura, H., Nishiyama, Y., and Tsurumi, T
  • Journal Title: J Virol Volume: 85(13) Pages: 6127-35
  • Peer Reviewed
• [Journal Article] Identification and Characterization of CCAAT Enhancer-binding Protein (C/EBP) as a Transoriptional Activator for Epstein-Barr Virus Oncogene Latent Membrane Prctein 1 (2011)
  • Author(s): Noda C
  • Journal Title: Journal of Biological Chemistry Volume: 286 Pages: 42524-33
  • Peer Reviewed
• [Journal Article] Involvement of Jun Dimerization Protein 2 (JDP2) in the Maintenance of Epstein-Barr Virus Latency (2011)
  • Author(s): Murata T
  • Journal Title: Journal of Biological Chemistry Volume: 286 Pages: 22007-16
  • Peer Reviewed
• [Journal Article] Tetrameric ring formation of Epstein-Barr virus polymerase processivity factor is crucial for viral replication (2010)
  • Author(s): Nakayama, S., Murata, T., Yasui, Y., Murayama, K., Isomura, H., Kanda, T., and Tsurumi, T
  • Journal Title: J Virol Volume: 84(24) Pages: 12589-98
  • Peer Reviewed
• [Journal Article] The human cytomegalovirus UL76 gene regulates the level of expression of the UL77 gene (2010)
  • Author(s): Isomura, H., Stinski, M. F., Murata, T., Nakayama, S., Chiba, S., Akatsuka, Y., Kanda, T., and Tsurumi, T
  • Journal Title: PLoS One Volume: 5(7)
  • Peer Reviewed
• [Journal Article] Transcriptional repression by sumoylation of Epstein-Barr virus BZLF1 protein correlates with association of histone deacetylase (2010)
  • Author(s): Murata, T., Hotta, N., Toyama, S., Nakayama, S., Chiba, S., Isomura, H., Ohshima, T., Kanda, T., and Tsurumi, T
  • Journal Title: J Biol Chem Volume: 285(31) Pages: 23925-35
  • Peer Reviewed
• [Journal Article] Transient increases in p53-responsible gene expression at early stages of Epstein-Barr virus productive replication (2010)
  • Author(s): Sato, Y., Shirata, N., Murata, T., Nakasu, S., Kudoh, A., Iwahori, S., Nakayama, S., Chiba, S., Isomura, H., Kanda, T., and Tsurumi, T
  • Journal Title: Cell Cycle Volume: 9(4) Pages: 807-14
  • Peer Reviewed
• [Journal Article] Tetrameric ring formation of Epstein-Barr virus polymerase processivity factor is crucial for viral replication (2010)
  • Author(s): Nakayama S
  • Journal Title: Journal of Virology Volume: 84 Pages: 12589-98
  • Peer Reviewed
• [Journal Article] Transcriptional repression by sumoylation of Epstein-Barr virus BZLF1 protein correlates with association of histone deacetylase (2010)
  • Author(s): Murata T
  • Journal Title: Journal of Biological Chemistry Volume: 285 Pages: 23925-35
  • Peer Reviewed
• [Presentation] Involvement of Jun Dimerization Protein 2(JDP2) in the Maintenance of Epstein-Barr virus Latency (2011)
  • Author(s): 村田貴之、鶴見達也
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-04
• [Presentation] Involvement of Jun Dimerization Protein 2 (JDP2) in the Maintenance of Epstein-Barr virus Latency (2011)
  • Author(s): Murata T
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場、名古屋
  • Year and Date: 2011-10-04
• [Presentation] Involvement of Jun Dimerization Protein 2(JDP2) in the Maintenance of Epstein-Barr virus Latency (2011)
  • Author(s): Murata T, Tsurumi T
  • Organizer: International Union of Microbiological Societies(IUMS) 2011, XV International Congress of Virology
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-12
• [Presentation] Involvement of Jun Dimerization Protein 2 (JDP2) in the Maintenance of Epstein-Barr virus Latency (2011)
  • Author(s): Murata T
  • Organizer: International Union of Microbiological Societies (IUMS) 2011, XV International Congress of Virology
  • Place of Presentation: 札幌コンベンションセンター、札幌
  • Year and Date: 2011-09-12
• [Presentation] Identification and Characterization of a Novel Transcriptional Activator for EBV Oncogene LMP1 in Epithelial Cells (2011)
  • Author(s): Murata T, Noda C, Kanda T, Tsurumi T
  • Organizer: 5th International Symposium on Nasopharyngeal Carcinoma
  • Place of Presentation: Penang, Malaysia
  • Year and Date: 2011-06-23
• [Presentation] Identification and Characterization of a Novel Transcriptional Activator for EBV Oncogene LMP1 in Epithelial Cells (2011)
  • Author(s): Murata T
  • Organizer: 5th International Symposium on Nasopharyngeal Carcinoma
  • Place of Presentation: Rasa Sayang Hotel、Penang, Malaysia
  • Year and Date: 2011-06-23
• [Presentation] EBV再活性化の分子メカニズム (2010)
  • Author(s): 村田貴之、鶴見達也
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
  • Year and Date: 2010-11-08
• [Presentation] EBV再活性化の分子メカニズム (2010)
  • Author(s): 村田貴之
  • Organizer: 第58回日本ウイルス学会学術総会
  • Place of Presentation: あわぎんホール(徳島県)
  • Year and Date: 2010-11-08
• [Presentation] EBウイルスの再活性化を誘導する細胞性因子のスクリーニング (2010)
  • Author(s): 村田貴之
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2010-09-22
• [Presentation] Screening of cellular factors that enhance reactivation of Epstein-Barr virus from latency (2010)
  • Author(s): Murata T, Tsurumi T
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-12
• [Presentation] Screening of cellular factors that enhance reactivation from Epstein-Barr virus latency (2010)
  • Author(s): Murata T, Tsurumi T
  • Organizer: 14th Biennial Conference of the International Association for Research on Epstein-Barr Virus and Associated Diseases
  • Place of Presentation: Birmingham, UK
  • Year and Date: 2010-09-07
• [Presentation] Screening of cellular factors that enhance reactivation from the EBV latency (2010)
  • Author(s): Murata T
  • Organizer: The 14^<th> Biennial Conference of the International Association for Research on EBV & Associated Diseases
  • Place of Presentation: バーミンガム大学(英国)
  • Year and Date: 2010-09-07
• [Remarks]
  • URL: http://www.pref.aichi.jp/cancer-center/ri/01bumon/06shuyo_uirusu/index.html
• [Remarks]
  • URL: http://www.pref.aichi.jp/cancer-center/ri/01bumon/06shuyo_uirusu/index.html
• [Remarks]
  • URL: http://www.pref.aichi.jp/cancer-center/400/420/421/421-06.html